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  • 1
    ISSN: 1432-072X
    Keywords: Methanobacterium ; Ammonia assimilation enzymes ; GS-GOGAT pathway ; NH + 4 regulation ; M. ivanovii glutamine auxotrophs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Various enzymes involved in the initial metabolic pathway for ammonia assimilation by Methanobacterium ivanovii were examined. M. ivanovii showed significant activity of glutamine synthetase (GS). Glutamate synthase (GOGAT) and alanine dehydrogenase (ADH) were present, wheras, glutamate dehydrogenase (GDH) was not detected. When M. ivanovii was grown with different levels of NH + 4 (i.e. 2, 20 or 200 mM), GS, GOGAT and ADH activities varied in response to NH + 4 concentration. ADH was not detected at 2 mM level, but its activity increased with increased levels of NH + 4 in the medium. Both GS and GOGAT activities increased with decreasing concentrations of NH + 4 and were maximum when ammonia was limiting, suggesting that at low NH + 4 levels, GS and GOGAT are responsible for ammonia assimilation and at higher NH + 4 levels, ADH might play a role. Metabolic mutants of M. ivanovii that were auxotrophic for glutamine were obtained and analyzed for GS activity. Results indicate two categories of mutants: i) GS-deficient auxotrophic mutants and ii) GS-impaired auxotrophic mutants.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 2 (1986), S. 309-317 
    ISSN: 1573-0972
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Résumé Dans les digesteurs anaérobies produisant du biogaz à partir de fumier de bovins, la production d'acétate, qui est l'étape essentielle conduisant au méthane, est limitée à cause de la nature ligno-cellulosique de la matière première. Il a été procédé à des prelévements à différentes profondeurs dans un digesteur KVIC en service, et à 110 isolements de bactéries cellulolytiques appartenant aux Bacillaceae, Propionibacteriaceae, Lactobacillaceae et bactéries coryneformes du typeCellulomonas. Les populations de bactéries cellulolytiques dénombrées varient entre 2,0×103 et 1,4×105 par g. d'échantillon. Certaines des bactéries isolées ont une activité cellulase plus élevée que d'autres, et dans tous les cas l'acétate est le produit prédominant. D'une manière générale, l'acétate, le propionate et le butyrate constituent les produits principaux. Une souche est homo-acétogène. Un petit nombre d'autres produisent, en dehors de l'acétate, de faibles quantités de propionate, de butyrate et d'isobutyrate. La vitesse de dégradation de la cellulose dans le digesteur dépend du rapport cellulose/lignine.
    Abstract: Resumen La producción de acetato, substancia clave para la formación de metano en digestores anaerobios, es el factor limitante en las plantas de biogás, alimentadas mediante residuos de ganado, debido a la naturaleza lignocelulósica del material de partida. La velocidad de hidrolisis de la celulosa afecta a la producción de acetato, sin embargo, nose conocen ni el tipo ni la cantidad de bacterias que existen en una planta de biogas de dichas características. Se aislaron 110 cepas bacterianas con propiedades celulolíticas que pertenecían a los grupos de Bacillus, Propionibacterias, y coryneformes, (como el géneroCellulomonas). Estudiando la población de bacterias celulolíticas a distintas profundidades de un digestor de biogás del tipo 103 KVIC, se observó que ésta variaba entre 2.103 y 1.4 103 celulas/g de residuo liquido analizado. Algunos de los aislados bacterianos mostraron una mayor actividad celulolitica que otros, siendo el acetato el producto final mayoritario. En general los productos finales más corrientes fueron: acetato, butirato y propionato; sin embargo, una de las cepas aisladas era homoacetogénica y algunas otras produjeron pequeñas cantidades de propionato, butirato oisibutirato junto con acetato. La velocidad de degradación de la celulosa dependía de la relación celulosa: lignina.
    Notes: Summary Production of acetate, the key substrate for methane formation in anaerobic digesters, is limiting in cattle waste-fed biogas digesters due to the lignocellulosic nature of the substrate. Since the rate of cellulose hydrolysis affects acetate production, and the number and type of cellulolytic bacteria that exist in a cattle waste biogas digester is not known, 110 cellulolytic bacterial isolates belonging to the Bacillaceae, Propionibacteriaceae and Lactobacillaceae, and coryneform bacteria likeCellulomonas, were isolated from different depths of an operating 103 KVIC type biogas digester. The population of cellulolytic bacteria varied between 2.0×103 and 1.4×105/g slurry. Some bacterial isolates exhibited higher cellulase activity than others, with acetate as the major end-product. In general, acetate, propionate and butyrate were the common end-products but one isolate was homoacetogenic and a few others produced propionate or butyrate orisobutyrate in small amounts with acetate. Cellulose degradation rate in the digester was dependent upon the cellulose: lignin ratio.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    BIT 28 (1988), S. 302-307 
    ISSN: 1572-9125
    Keywords: AMS(MOS) ; 65L05 ; Nonlinearly damped oscillators ; numerical solution ; general second order differential equation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We report a modification of the Stiefel-Bettis method which is of trigonometric order one and of polynomial order two for the general second order initial value problems. We also discuss the modified Stiefel-Bettis method made explicit for the undamped nonlinear oscillators. Numerical solution of problems are given to illustrate the methods.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 22 (1987), S. 3864-3872 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The effects of stabilization conditions on the formation of a consolidated carbon fibre structure from two acrylonitrile-based precursor fibres, one containing itaconic acid as comonomer and the other a commercial precursor, have been studied. The progression of changes in elemental composition and properties such as sonic modulus, electrical resistance and density in a continuous, low temperature (1200° C) carbonization process are reported for the first time. A criterion based on attaining a composition dependent critical density in stabilization is proposed for avoiding the formation of a hollow core in carbon fibres processed continuously at reasonably rapid rates. Aspects related to the development of open and closed micropores in the carbon fibre structure and the possible mechanisms for the formation of a hollow core in carbonization are also discussed.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    BIT 25 (1985), S. 250-255 
    ISSN: 1572-9125
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We have derived a two-stage implicit fifth order multipoint iterative method for solving equations. Further, we have also obtained an implicit third order and a semiexplicit fourth order multipoint method. Comparison of computational results are made with other well-known methods on a number of difficult problems. The implicit multipoint methods are accurate and robust.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 329 (1987), S. 162-164 
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Numerical Methods for Partial Differential Equations 5 (1989), S. 87-95 
    ISSN: 0749-159X
    Keywords: Mathematics and Statistics ; Numerical Methods
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics
    Notes: We present a nine-point fourth-order finite difference method for the nonlinear second-order elliptic differential equation Auxx + Buyy = f(x, y, u, ux, uy) on a rectangular region R subject to Dirichlet boundary conditions u(x, y) = g(x, y) on ∂R. We establish, under appropriate conditions O(h4)-convergence of the finite difference scheme. Numerical examples are given to illustrate the method and its fourth-order convergence.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Publication Date: 1986-05-01
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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  • 9
    Publication Date: 1987-11-01
    Print ISSN: 0022-2461
    Electronic ISSN: 1573-4803
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Published by Springer
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  • 10
    Publication Date: 1987-08-12
    Print ISSN: 0108-7673
    Electronic ISSN: 2053-2733
    Topics: Chemistry and Pharmacology , Geosciences , Physics
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