ISSN:
1432-1955
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Electrophoresis on SDS gel and analytical isoelectric focusing showed that a crude extract ofAngiostrongylus cantonensis consisted of at least 40 protein components with molecular weights ranging from 13000–70000 and isoelectric points of pI values ranging from 3.7–10.0. Crossed-immunoelectrophoresis with a hyperimmune antiserum toA. cantonensis showed at least 40 different antigenic components in the crude worm extract which were cross-reactive with those ofAscaris suum, Metastrongylus apri andToxocara canis. Using preparative isoelectric focusing, the somatic worm preparation was divided into 13 equal fractions, of which 3, 4 and 5, with pI values of 3.7, 4.0 and 4.45 respectively, were later shown by immunoelectrophorectic techniques and enzymelinked immunosorbent assay to contain antigens specific toA. cantomensis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00926804
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