Publication Date:
1988-09-16
Description:
An in vitro assay was developed to study the positive factors that regulate the onset of DNA replication during the mammalian cell cycle. Extracts prepared from cells at defined positions in the cell cycle were used to examine the replication of SV40 DNA in a cell free system. Extracts prepared from S phase cells were ten times more efficient at initiating replication at the SV40 origin than were extracts from G1 cells, whereas elongation rates were similar in G1 and S reactions. At a discrete point in the cell cycle, just before the cell's entry into S, an activity appeared that was required, in conjunction with SV40 T antigen, for site specific initiation at the SV40 origin. This factor had a role in unwinding DNA at the replication origin.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Roberts, J M -- D'Urso, G -- AG0005/AG/NIA NIH HHS/ -- New York, N.Y. -- Science. 1988 Sep 16;241(4872):1486-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Fred Hutchinson Cancer Research Center, Seattle, WA 98104.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2843984" target="_blank"〉PubMed〈/a〉
Keywords:
Antigens, Polyomavirus Transforming/physiology
;
*Cell Cycle
;
Cell Line
;
Cell-Free System
;
*DNA Replication
;
Humans
;
In Vitro Techniques
;
Interphase
;
Simian virus 40/genetics
;
Virus Replication
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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