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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 49 (1989), S. 301-305 
    ISSN: 1432-0649
    Keywords: 82.50 ; 81.15
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We have studied the photolysis of molybdenum hexacarbonyl by focused excimer laser radiation. It was found that electronically excited Mo atoms detected in the focus region of a KrF laser are due to a direct two photon absorption transition. The upper limit of the energy for complete dissociation of Mo(CO)6 has been derived from these results. Two photon dissociation in the gas phase should be the dominant process at metal film deposition on substrates positioned perpendicularly and near to the focus. Adding buffer gases to the organometallic vapor particle formation was observed in the whole irradiated gas volume. The analysis of scattered He-Ne laser light yields information about the density and size of these particles. Some conclusions are drawn with respect to structured metal film deposition with high spatial selectivity.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 98 (1989), S. 0 
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: We attempted to develop a direct time-domain, finite-difference solution for the electromagnetic response of a 3-D model. The algorithm is an extension of our 2-D modelling technique, which uses the Du Fort-Frankel finite-difference scheme. However, the vector nature of the field makes the 3-D problem much more complicated than its 2-D counterpart, and a supercomputer is required for computations. Unlike the 2-D case, where we solve for the electric field, the solution is formulated in terms of secondary magnetic field to avoid dealing with a discontinuous normal component of electric field. However, that difficulty is replaced with a problem involving the gradient of conductivity, which is discontinuous at interfaces. We experimented with both smoothing the conductivity variation, so that the gradient is well defined; and integrating the gradient terms, which results in a tangential current density contrast. Our limited experiments indicate that the magnetic field step response is computationally more stable than the impulse response, because it is a smoother function with smaller dynamic range. However, the step response takes longer to compute because its source, which involves the primary fields in the earth, requires very accurate numerical integration. Due to computer time and memory limitations on the supercomputer available to us, we were not able to develop an accurate numerical solution. We were able to carry out only a few tests on small models for which results were not in good agreement with values computed using a 3-D integral equation solution.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 11 (1986), S. 217-225 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Gene cloning ; Invertase genes ; Multicopy vector
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Six unlinked loci for invertase structural genes are known in the yeast Saccharomyces cerevisiae: SUC1-SUC5 and SUC7. These genes are similar in structure and expression but not identical. Different yeast strains possess none, one or several of these genes. We have isolated the genes SUC1-SUC5, subcloned them into the multicopy vector YEp24 and compared the expression of the five SUC genes in one recipient strain. SUC2 was isolated by transformation of a suc0 strain with a gene pool and complementation to sucrose fermentation. SUC4 was cloned from a minipool of chromosomal fragments which were shown to contain SUC4 by Southern hybridization. SUC1, SUC3 and SUC5 were isolated using the method of plasmid eviction. A plasmid containing regions flanking SUC4 was integrated next to these SUC genes. The plasmid together with the SUC genes were then cut out of the chromosome using an appropriate restriction endonuclease. The length of chromosomal DNA fragments containing the different SUC genes were 4.8 kb for SUC1, 5.2 kb for SUC2, 4.8 kb for SUC3, 12.8 kb for SUC4 and 17.2 kb for SUC5. Fragments containing the complete SUC genes and the sequences controlling their expression were subcloned into YEp24 and transformed into a strain without any active invertase gene. Invertase activity of transformants was measured after growth repressing (8% glucose) and derepressing (2% raffinose) conditions. As expected from results with strains carrying the individual SUC genes in a chromosomal location, the SUC genes were expressed to a different extent.
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  • 4
    ISSN: 1432-0983
    Keywords: Alcoholic fermentation ; Deletion mutant ; Pyruvate decarboxylase ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We deleted most of the pyruvate decarboxylase structural gene PDC1 from the genome of Saccharomyces cerevisiae. Surprisingly, mutants carrying this deletion allele showed a completely different phenotype than previously described point mutations. They were able to ferment glucose and their specific pyruvate decarboxylase activity was only reduced to 45% of the wild type level. Northern blot analysis revealed that a sequence in the yeast genome homologous to PDC1 and formerly designated as a possible pseudogene is expressed and may code for a different but closely related pyruvate decarboxylase. The products of the two PDC genes seem to form hybrid oligomers, however both homooligomers have enzyme activity. Thus, the product of the PDC1 gene is not absolutely neccessary for glucose fermentation in yeast.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 12 (1987), S. 519-526 
    ISSN: 1432-0983
    Keywords: Yeast transformation ; Multiple integration ; Enzyme overproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Integrative transformation of yeast with gapped DNA fragments results in single or multiple integration into the yeast genome via homologous recombination. A sequence of yeast DNA was found which favours multiple integration even when the strategy of gene replacement is used. This strategy by which the transformed DNA fragment replaces its chromosomal homologue rather than simply integrating into the genome usually occurs as a single exchange event. The described region is unique and lies ear a telomere about 5 kb proximal to the SUC4 locus on chromosome XIII. DNA from this region was used as a vehicle for the integration of different SUC genes coding for invertase. Most of the sucrose fermenting transformants isolated carried between two and seven copies of the SUC genes. These transformants overproduced invertase even though there was no selective pressure for high invertase activity in these experiments. I conclude that this region is highly recombinogenic and favours multiple integration of DNA fragments. This region could be used for stable multiple integration of heterologous genes into the yeast genome for over-production of the respective gene product.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Primates 29 (1988), S. 565-567 
    ISSN: 0032-8332
    Keywords: Liontailed macaques ; Macaca silenus ; Tool use ; Food preparation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three members of a group of liontailed macaques (Macaca silenus) were seen to use leaves for food preparation. Other examples of prey-selection and hunting behaviour in liontailed macaques reflect individual- and group-specific skills. The absence of similar patterns in bonnet macaques (Macaca radiata) living in the same habitat might be related to differences in the social design and indicate the high significance of social aspects for the occurrence and manifestation of innovative behaviour.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Primates 30 (1989), S. 325-345 
    ISSN: 0032-8332
    Keywords: Bonnet macaques ; Macaca radiata ; Vocal repertoire ; Vocal behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Field observations and spectrographic analyses of sound recordings of South Indian bonnet macaques revealed a vocal repertoire of at least 25 basic patterns. The repertoire consists of well separated sound classes and acoustic categories connected by structural intergradation. Besides structural variations within and between different elements of the repertoire, the vocal system ofMacaca radiata is characterized by regular combinations of particular basic patterns. These combinations occurred not only between calls of similar structure and function but also between calls usually emitted in entirely different social contexts. According to the qualitative analysis, sex-specific asymmetries of the vocal behaviour were less pronounced than age-dependent characteristics. The comparison of clear call vocalizations ofMacaca radiata andM. fuscata revealed significant species-specific differences on the structural and the behavioural level. Evaluations of the structural features of alarm calls of various macaque species imply marked differences between members of thefascicularis group andsinica group on one hand and thesilenus group andarctoides group on the other.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 211 (1988), S. 446-454 
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; Invertase genes ; Promoter sequences ; Palindromes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the yeast Saccharomyces cerevisiae six unlinked structural genes for invertase, the SUC genes, are known. We sequenced about 800 bp of the 5′ non-coding region and the first 220 bp of the coding region of the genes SUC1, SUC3, SUC4 and SUC5 and compared them with the previously sequenced genes SUC2 and SUC7 (Sarokin and Carlson 1985a). All are highly homologous within the coding region but in the non-coding region SUC1 shows some differences and SUC2 is more highly diverged. Two different kinds of TATA boxes were identified: the more strongly expressed genes SUC1, 2 and 4 have the sequence TATAAA and the more weakly expressed genes SUC3, 5 and 7 have TACAAA. Though the SUC1 sequence is in general more homologous to the other SUC genes, the region between-140 and + 100 of SUC1 is nearly identical to SUC2. This could be due to a gene conversion between SUC1 and the silent suc2 o allele which occurs in the strains carrying SUC1. Within the upstream regions of all the SUC genes three regions with palindromic sequences analogous to stem and loop structures were identified. Comparable structure could be detected in similar positions in the upstream sequences of the divergently transcribed yeast gene pairs MAL6S-MAL6T and GAL1-GAL10. Implications for the importance of these structures in the regulation and initiation of transcription are discussed.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 216 (1989), S. 511-516 
    ISSN: 1617-4623
    Keywords: Nonsense mutation ; Read-through ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The yeast invertase structural gene SUC2 has two naturally occurring alleles, the active one and a silent allele called suc2°. Strains carrying suc2° are unable to ferment sucrose and do not show detectable invertase activity. We have isolated suc2° and found an amber codon at position 232 of 532 amino acids. However, transformants carrying suc2° on a multicopy plasmid were able to ferment sucrose and showed detectable invertase activity. Full-length invertase was found in gels stained for active invertase and in immunoblots. Therefore we concluded that the amber codon is occasionally read as an amino acid. The calculated frequency of read-through is about 4% of all translation events.
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  • 10
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 19 (1989), S. 149-156 
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Wie kann eine Zelle je nach ihren Bedürfnissen bestimmte Gene an- oder abschalten? Ob ein bestimmtes Gen in mRNA umgeschrieben wird, entscheidet sich bei der Initiation der Transkription, dem wichtigsten Angriffspunkt für Regulationsmechanismen. Diese Mechanismen scheinen in einer Hefezelle, die sich mit der Notwendigkeit zum Abbau von Galaktose konfrontiert sieht, im Prinzip dieselben zu sein wie in einer tierischen Zelle im vielzelligen Organismus, die sich für eine Differenzierung entscheiden muß oder die auf ein Hormon mit der Synthese bestimmter Proteine zu reagieren hat.
    Additional Material: 8 Ill.
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