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  • 1
    Electronic Resource
    Electronic Resource
    Response of a Fabry–Perot cavity to phase modulated light (1987)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 58 (1987), S. 1406-1412 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: We present a solution to the lock-in detection method which is valid for arbitrary values of the modulation frequency. Our solutions agree with the results of the quasistatic theory, in the limit of small modulation frequency compared to the resonance linewidth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1139422
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  • 2
    Electronic Resource
    Electronic Resource
    Erratum: Response of a Fabry–Perot cavity to phase modulated light [Rev. Sci. Instrum. 58, 1406 (1987)] (1987)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 58 (1987), S. 2345-2345 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1139669
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  • 3
    Electronic Resource
    Electronic Resource
    Practical sound-reducing enclosure for laboratory use (1986)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 57 (1986), S. 2532-2534 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: We describe the design of a sound-reducing laboratory enclosure. The unit fits directly over the experiment and is hoisted to the ceiling during setup and adjustment stages. The advantages of the design are its modest cost, saving of space, and the fact that no door is required. The average sound isolation achieved is 30 dB, typical for a wall mass per unit area of 35 kg/m2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1139105
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of applied nitrogen upon aspects of nitrogen metabolism and transport in developing nodulated winged bean plants (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 77 (1989), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Nodulated winged bean [Psophocarpus tetragonolobus (L.) DC., cv. UPS 122] were grown under constant environmental conditions and supplied with mineral nutrient solution in which nitrogen was absent or was present as nitrate (12 mg N week-1 plant-1).Nitrate treatment dramatically promoted plant growth, increased fruit weight 1.6 fold, was necessary for tuberisation and enhanced nodulation. The in vitro accumulation of 14C into asparagine and aspartate components of excised nodules supplied with exogenous 14CO2 and [14C]-D-glucose was greater for nitrate-treated plants, whilst accumulation into ureides was reduced by nitrate treatment. Levels of amino acids in xylem sap were greater for plants supplied with a complete nutrient solution, than those grown without applied nitrate, particularly for asparagine, glutamine and proline. Xylem ureide levels were greater for plants grown in the absence of supplementary nitrate.Nitrogen accumulated in leaf, stem and petiole, and root nodule tissues for utilisation during fruit development; peak nitrogen levels and time of anthesis were retarded for plants grown without applied nitrate. The shoot ureide content increased during fruiting, coincident with decreases in the total nitrogen content, indicating that ureide pools are not utilised during the early reproductive phase. However ureide reserves, particularly allantoin, were utilised during the later stages of pod fill.Enzyme activity which metabolised asparagine was found throughout the plant and was identified as K+-dependent asparaginase (EC 3.5.1.1) and an aminotransferase. Apart from temporal differences in developmental profiles of enzyme activity, the activity of these enzymes and of allantoinase (EC 3.5.2.5) in developing tissues were similar for both treatments. The main differences were greater asparaginase and asparagine:pyruvate aminotransferase activities in root tissues and fruit of nitrate-supplied plants; allantoinase activity in the primary roots of plants grown without nitrate decreased during development, whilst activity in developing tubers (nitrate-supplied plants) increased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1989.tb05654.x
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  • 5
    Electronic Resource
    Electronic Resource
    Cytometric aspects of the leaves of the halophyte Suaeda maritima (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 64 (1985), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An analysis of the frequency of occurrence of chloroplasts, mitochondria and microbodies in leaf cells of the halophyte Sueda maritima (L.) Dum, showed a remarkable increase (almost a doubling) in the number of mitochondria per cell section when plants were grown in saline (340 mol m−3 NaCl) as opposed to non-saline conditions. Chloroplast and microbody volume per cell increased under saline conditions, but number per cell was insensitive to salt. Chloroplasts contained a greater number of starch grains in plants grown under saline as compared to non-saline conditions and, under both conditions, were closely associated with the cell wall adjacent to the intercellular spaces. The implications for growth, photosynthesis and respiration are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1985.tb03354.x
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  • 6
    Electronic Resource
    Electronic Resource
    The role of cell wall synthesis in sustained auxin-induced growth (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 63 (1985), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The dependence of auxin-induced growth on continued cell wall synthesis was investigated in stem segments of etiolated pea (Pisum sativum L. cv. Alaska) seedlings using the cell wall synthesis inhibitors monensin and 2,6-dichlorobenzonitrile (DCB). Monensin (5 μM) potently inhibited indole-3-acetic acid (IAA)-induced growth, particularly during the second hour of treatment, whereas growth in fusicoccin (FC) was inhibited much less effectively. Incorporation of [14C]-glucose into both matrix and cellulose fractions of the wall showed a sharp increase beginning after about 60 min, this rise being promoted by both IAA and FC. Monensin inhibited this rise in incorporation of label and completely removed the promotion of this by IAA, although some promotion by FC remained. Monensin inhibited incorporation into cellulose in a manner similar to that into matrix, but the use of the apparently specific cellulose synthesis inhibitor DCB showed that cellulose synthesis could be strongly inhibited without effect on growth, at least in the short term. The results support the view that sustained auxin-induced growth depends upon the incorporation of new matrix cell wall components into the wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1985.tb02318.x
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  • 7
    Electronic Resource
    Electronic Resource
    Rapid cellular responses to auxin and the regulation of growth (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 10 (1987), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The cellular responses rapidly evoked by auxin are reviewed, and related to a consideration of how growth rate is regulated in excised segments and in whole dicotyledonous plants. Two processes, synthesis of proteins and of cell wall components, are both promoted by auxin and essential for auxin-stimulated growth, whereas other processes show little promotion by auxin or do not appear essential for growth. Current models for the cellular regulation of growth by auxin are briefly discussed, and a new model presented. Auxin is suggested to act by bringing about a transient increase in cytosolic Ca2+ levels, which through the stimulation of protein kinases converts a cytoplasmic protein factor to an active state capable of binding auxin. The protein-auxin complex induces mRNA synthesis, which effects the increased synthesis of cell wall components and their incorporation into the wall, resulting in wall loosening and growth. It is proposed that the factor limiting growth in floating excised segments may initially be cell wall pH, but that this is not the case in whole plants and growth is instead mediated by increased protein and matrix cell wall synthesis. Differences are noted between monocotyledonous coleoptiles and dicotyledonous stems in some metabolic processes possibly involved in auxin growth responses, and it is cautioned that observations made on one tissue may not necessarily be applicable to the other. Care should also be taken in applying conclusions drawn from studies on excised tissue to the interpretation of growth regulation in the whole plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/1365-3040.ep11603716
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  • 8
    Electronic Resource
    Electronic Resource
    The regulation of turgor pressure during sucrose mobilisation and salt accumulation by excised storage-root tissue of red beet (1987)
    Springer
    Planta 170 (1987), S. 353-361 
    Details
    ISSN: 1432-2048
    Keywords: Beta (storage root) ; Osmotic pressure ; Salt uptake ; Sucrose mobilisation ; Turgor pressure ; Vacuole (solute content)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The changes in turgor pressure that accompany the mobilisation of sucrose and accumulation of salts by excised disks of storage-root tissue of red beet (Beta vulgaris L.) have been investigated. Disks were washed in solutions containing mannitol until all of their sucrose had disappeared and then were transferred to solutions containing 5 mol·m-3 KCl+5 mol·m-3 NaCl in addition to the mannitol. Changes in solute contents, osmotic pressure and turgor pressure (measured with a pressure probe) were followed. As sucrose disappeared from the tissue, reducing sugars were accumulated. For disks in 200 mol·m-3 mannitol, the final reducing-sugar concentration equalled the initial sucrose concentration so there was no change in osmotic pressure or turgor pressure. At lower mannitol concentrations, there was a decrease in tissue osmotic pressure which was caused by a turgor-driven leakage of solutes. At concentrations of mannitol greater than 200 mol·m-3, osmotic pressure and turgor pressure increased because reducing-sugar accumulation exceeded the initial sucrose concentration. When salts were provided they were absorbed by the tissue and reducing-sugar concentrations fell. This indicated that salts were replacing sugars in the vacuole and releasing them for metabolism. The changes in salf and sugar concentrations were not equal because there was an increase in osmotic pressure and turgor pressure. The amount of salt absorbed was not affected by the external mannitol concentration, indicating that turgor pressure did not affect this process. The implications of the results for the control of turgor pressure during the mobilisation of vacuolar sucrose are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395027
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  • 9
    Electronic Resource
    Electronic Resource
    A quantitative stereological analysis of the effect of indoleacetic acid on the dictyosomes in pea stem epidermal cells (1985)
    Springer
    Protoplasma 125 (1985), S. 230-234 
    Details
    ISSN: 1615-6102
    Keywords: Dictyosomes ; Indoleacetic acid ; Pisum sativum ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A quantitative analysis of electron micrographs showed that IAA treatment caused an initial rapid increase in the amount of dictyosome material in pea stem epidermal cells. The increase was detected within 15 minutes of auxin presentation and reached a maximum around 30 minutes. This was followed by a decrease, presumably due to an increased utilization of the organelle. The decrease involved a fall in the amount of dictyosome-derived vesicles and in the actual number of dictyosomes. The results are discussed in relation to similar observations on expanding cells of monocotyledonous plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281242
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  • 10
    Electronic Resource
    Electronic Resource
    The effect of calcium antagonists and inhibitors of secretory processes on auxin-induced elongation and fine structure ofPisum sativum stem segments (1986)
    Springer
    Protoplasma 133 (1986), S. 149-159 
    Details
    ISSN: 1615-6102
    Keywords: Calcium ; Cell elongation ; Indole-3-acetic acid ; Pisum sativum ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The treatment of dark grown pea stem segments with chelators of divalent cations (EGTA, EDTA, CTC), various Ca2+ antagonists (LaCl3, A-23187, verapamil) and inhibitors of secretory processes (monensin, CB) reduced elongation in the presence of indole-3-acetic acid (IAA). Generally the inhibition increased with increasing concentrations of the substances. The timing of the responses can be correlated with maximum auxin-stimulated secretion of cell wall material. Examination of cell ultrastructure showed that changes in dictyosome activity could explain a reduced deposition of cell wall material and so cause inhibition of elongation. The inhibitors affected the morphology and vesiculation of the dictyosomes, and the appearance of the plasma membrane, ER and mitochondria in different ways. The most pronounced effects on ultrastructure resulted from monensin and LaCl3 treatments with the dictyosomes being most affected; large vesicles appeared in the cytoplasm. Less pronounced effects on cell structure were seen in EGTA, A-23187 and verapamil treated tissue. The effects on the dictyosomes are considered to be due to disturbances of Ca2+ and other ionic levels within the cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01304630
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