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  • 1
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    Purification and properties of Drosophila heat shock activator protein (1987)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1987-11-27
    Description: Drosophila heat shock activator protein, a rare transacting factor which is induced upon heat shock to bind specifically to the heat shock regulatory sequence in vivo, has been purified from shocked cells to more than 95 percent homogeneity by sequence-specific duplex oligonucleotide affinity chromatography. The purified protein has a relative molecular mass of 110 kilodaltons, binds to the regulatory sequence with great affinity and specificity, and strongly stimulates transcription of the Drosophila hsp70 gene. Studies with this regulatory protein should lead to an understanding of the biochemical pathway underlying the heat shock phenomenon.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wu, C -- Wilson, S -- Walker, B -- Dawid, I -- Paisley, T -- Zimarino, V -- Ueda, H -- New York, N.Y. -- Science. 1987 Nov 27;238(4831):1247-53.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3685975" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Base Sequence ; Drosophila/*genetics ; *Genes ; *Genes, Regulator ; Heat-Shock Proteins/*genetics ; Kinetics ; Molecular Weight
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
    Electronic Resource
    Electronic Resource
    ZnSe homoepitaxial layers grown at very low temperature by atmospheric pressure metalorganic vapor-phase epitaxy (1989)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 65 (1989), S. 2728-2733 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: ZnSe homoepitaxial layers with high crystalline quality were grown on (100) ZnSe substrates (grown by the iodine vapor transport method) between 190 and 250 °C. The surface morphologies of homoepitaxial layers (homoepilayers) grown below 250 °C were all mirrorlike. The concentration of self-activated (SA) centers is related to the growth temperature. The band-edge photoluminescence (PL) intensity increases drastically with increasing temperature from 190 up to 210 °C, decreases above 210 °C, and the SA-PL intensity increases monotonically as the growth temperature increases. The mechanism of suppressing the occurrence of SA centers below 250 °C is assumed that the concentration of Zn vacancies diffused from the highly iodine-doped ZnSe substrates into the ZnSe homoepilayers reduces at this temperature region. The SA-PL intensity of the ZnSe homoepilayer with the strongest band-edge emission (grown at 210 °C) is the same or weaker than that of the best ZnSe heteroepitaxial layer (heteroepilayer). Below 200 °C, the FWHM of the ZnSe (400) diffraction is 14 arcsec which is the best value we have ever obtained, though the growth temperature seems to be too low to maintain the crystalline quality. The comparison on the crystalline quality with the ZnSe heteroepilayers is discussed in detail from the viewpoints of the epitaxial growth mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.342760
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  • 3
    Electronic Resource
    Electronic Resource
    Structure of p-aminobenzoic acidi–1,3-dimethyl-2-imidazolidinone (1/1) (1986)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 42 (1986), S. 462-464 
    Details
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108270186095793
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  • 4
    Electronic Resource
    Electronic Resource
    Carnosine-like immunoreactivity in the primary olfactory neuron of the rat (1987)
    Springer
    Cellular and molecular life sciences 43 (1987), S. 298-300 
    Details
    ISSN: 1420-9071
    Keywords: Carnosine ; neurotransmitter ; olfactory bulb ; primary olfactory neuron ; immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using the peroxidase-antiperoxidase immunohistochemical technique, carnosine-like immunoreactivity was demonstrated to localize specifically within the primary olfactory neuron.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01945557
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular cloning of the fibroin light chain complementary DNA and its use in the study of the expression of the light chain gene in the posterior silk gland ofBombyx mori (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1167-1171 
    Details
    ISSN: 1420-9071
    Keywords: Silkworm ; Bombyx mori ; silk gland ; mRNA ; complementary DNA ; fibroin light chain ; molecular cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fibroin light chain (L-chain) mRNA (mol. wt 4.0×105 daltons) was purified from the posterior silk gland of the silkworm,Bombyx mori (J-131 strain). Double-stranded complementary DNA was synthesized and inserted into the PstI site of pBR322 employing the oligo(dC)-oligo(dG) tailing method. Several recombinant plasmids containing the inserts of about 800 base pairs were isolated. Hybridization-translation assay demonstrated that these clones hybridized specifically with the fibroin L-chain mRNA. One of these clones (pLA23) was used as a probe to investigate relative concentrations of the fibroin L-chain gene and mRNA in the posterior silk glands at different stages of late larval development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01951711
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  • 6
    Electronic Resource
    Electronic Resource
    An observational study of the structure of the nocturnal urban boundary layer (1988)
    Springer
    Boundary layer meteorology 45 (1988), S. 59-82 
    Details
    ISSN: 1573-1472
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The formation mechanism of the nocturnal urban boundary layer (UBL), especially in the winter nighttime, was investigated based on the extensive field observations conducted during November 1984 in Sapporo, Japan. A strong, elevated inversion formed over the Sapporo urban area and the inversion base height was approximately twice the average building height. Velocity fluctuations Σ u, Σw and Reynolds stress % MathType!MTEF!2!1!+-% feaafeart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaWaa0aaaeaaca% WG1bWaaWbaaSqabeaacaaIXaaaaGGaaOGae8hiaaIaam4DamaaCaaa% leqabaGaaGymaaaaaaaaaa!3A9C!\[\overline {u^1 w^1 } \] had nearly uniform profiles within the nocturnal UBL and decreased with height above the UBL. On the other hand, temperature fluctuations Σ t , and heat fluxes % MathType!MTEF!2!1!+-% feaafeart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaWaa0aaaeaaca% WG1bWaaWbaaSqabeaacaaIXaaaaGGaaOGae8hiaaIaeqiUde3aaWba% aSqabeaacaaIXaaaaaaaaaa!3B56!\[\overline {u^1 \theta ^1 } \] and % MathType!MTEF!2!1!+-% feaafeart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaWaa0aaaeaaca% WG3bWaaWbaaSqabeaacaaIXaaaaGGaaOGae8hiaaIaeqiUde3aaWba% aSqabeaacaaIXaaaaaaaaaa!3B58!\[\overline {w^1 \theta ^1 } \] had peaks at the inversion base and small values within the nocturnal UBL. The turbulent kinetic energy budget showed that the turbulent transport term and shear generation from urban canopy elements are important in the nocturnal UBL development; the role of the buoyancy term is small. The turbulence data analysis and application of a simple advective model showed that the mechanism of UBL formation may be controlled by the downward transport of sensible heat from the elevated inversion caused by mechanically-generated turbulence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00120815
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  • 7
    Electronic Resource
    Electronic Resource
    Water soluble cyclodextrin polymers: Their interaction with drugs (1987)
    Springer
    Journal of inclusion phenomena and macrocyclic chemistry 5 (1987), S. 427-431 
    Details
    ISSN: 1573-1111
    Keywords: Cyclodextrin polymer ; solubility method ; complex stability ; NMR spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The complex forming ability of a water-soluble β-cyclodextrin epichlorohydrin, polymer (CDPS) and its different molecular weight fractions was studied and compared with the complexing properties of β-cyclodextrin (βCD) and dimethyl-βCD (DM-βCD). CDPS was separated into two main fractions. CDPS and its fractions formed well soluble inclusion compounds with the studied drugs. The low molecular weight fraction formed rather stable complexes with small guest molecules, the high molecular weight fraction was found to be more efficient in binding larger substrates. Structural studies of furosemide-CD complexes were attempted by NMR spectroscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00664098
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  • 8
    Electronic Resource
    Electronic Resource
    Numerical modeling of the nocturnal urban boundary layer (1989)
    Springer
    Boundary layer meteorology 49 (1989), S. 77-98 
    Details
    ISSN: 1573-1472
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract A numerical case study with a second-order turbulence closure model is proposed to study the role of urban canopy layer (UCL) for the formation of the nocturnal urban boundary layer (UBL). The turbulent diffusion coefficient was determined from an algebraic stress model. The concept of urban building surface area density is proposed to represent the UCL. Calculated results were also compared with field observation data. The height of the elevated inversion above an urban center was simulated and found to be approximately twice the average building height. The turbulent kinetic energy k, energy dissipation rate ε, and turbulence intensities 〈u 2〉 and 〈w 2〉 increase rapidly at the upwind edge of the urban area. The Reynolds stress 〈uw〉 displayed a nearly uniform profile inside the UBL, and the vertical sensible heat flux 〈wθ〉 had a negative value at the inversion base height. This indicates that the downward transport of sensible heat from the inversion base may play an important role in the formation of the nocturnal UBL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00116406
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  • 9
    Electronic Resource
    Electronic Resource
    Characterization of mixtures of linear polyethylenes of ultrahigh and moderate molecular weights (1986)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 26 (1986), S. 1483-1488 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The crystallinity, density, dynamic mechanical properties, (25 to 250°C, 11 Hz), and tensile properties at 25°C have been studied for two mixtures of ultrahigh-molecular-weight linear polyethylene (Mw = 2.0 × 106), abbreviated UHMWLPE, and moderate-molecular-weight linear polyethylene (Mw = 5.7 × 104), abbreviated LPE. These mixtures, which covered the entire composition range, had been prepared with differing solvent-thermal histories. An enhancement of both crystallinity and density and an obvious synergism in tensile properties were observed for quenched films made from the mixture in which both components had been co-dissolved. The data indicate that UHMWLPE and LPE can cocrystallize under given circumstances.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760262104
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  • 10
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    Reconstitution of rat brain mu opioid receptors with purified guanine nucleotide-binding regulatory proteins, Gi and Go. (1988)
    National Academy of Sciences
    Details
    Publication Date: 1988-09-01
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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