ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract We investigated the in vitro effects of ions, carbohydrates, lectins, and charged compounds on the cytolytic and hemolytic action of the purified δ-endotoxin ofBacillus thuringiensis var.darmstadiensis 73-E10-2, and other δ-endotoxins. Cytotoxicity was inhibited by preincubating the toxin with N-acetylneuraminic, glucuronic, galacturonic, and N-acetylglutamic acids and ATP. Lectins were unable to inhibit toxicity. Pretreatment of sheep erythrocytes with neuraminidase enhanced hemolysis. These results suggested a nonspecific inhibition of cytotoxicity based on the presence of a negative charge. Supraphysiological concentrations of divalent cations such as Ca2+, Mg2+, and Zn2+ in the medium yielded a reduced toxicity, whereas EDTA and EGTA enhanced cytotoxicity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01589385
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