ISSN:
1432-072X
Keywords:
Oxidase regulation
;
Cytochrome c oxidase
;
Cytochrome c 2
;
Regulatory DNA sequence
;
Rhodobacter capsulatus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In order to distinguish between the regulatory effects of oxygen tension and light intensity on cytochrome c oxidase protein and enzymatic activity cells of Rhodobacter capsulatus were shifted from phototrophic (anaerobic, light) growth to aerobic-light, aerobic-dark and to anaerobic-dark conditions, respectively. During shift-experiments the formation of oxidase protein and regulation of oxidase activity was followed by immunological and enzymatic means. The results support the idea, that the formation of oxidase protein is regulated by oxygen tension and light intensity changes, whereas the regulation of oxidase activity seems only to be correlated to the oxygen tension. A DNA sequence involved in the oxygen-dependent regulation of cytochrome oxidase could be identified in the regulation-deficient oxidase mutant H41 of R. capsulatus. Immunological investigations of cytochrome c 2 from mutant H41 demonstrated at the same time the participation of the c 2-polypeptide in the regulation of cytochrome c oxidase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00423129
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