Publication Date:
1985-12-06
Description:
Sequences upstream from the proto-oncogene fos were shown to be essential for its transcription. Transient expression of the chloramphenicol acetyl-transferase (CAT) gene linked to upstream sequences of the fos gene including its promoter reveals that sequences located 64 to 404 base pairs 5' to the fos cap site contain a typical transcriptional enhancer. Moreover, these enhancer sequences, which are strikingly conserved between mouse and human fos genes, coincide with a deoxyribonuclease I-hypersensitive site in the chromatin. The expression of the fos-CAT fusion genes was stimulated only two to three times by the fos inducer 12-0-tetradecanoyl phorbol-13-acetate. The fos enhancer does not appear to be tissue-specific.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Deschamps, J -- Meijlink, F -- Verma, I M -- New York, N.Y. -- Science. 1985 Dec 6;230(4730):1174-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3865371" target="_blank"〉PubMed〈/a〉
Keywords:
Acetyltransferases/genetics
;
Animals
;
Chloramphenicol O-Acetyltransferase
;
Chromatin/metabolism
;
DNA, Recombinant
;
Deoxyribonuclease I/metabolism
;
*Enhancer Elements, Genetic
;
*Genes, Regulator
;
Humans
;
Mice
;
*Proto-Oncogenes
;
*Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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