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  • Springer  (205)
  • 1985-1989  (205)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Isolation of mutants sensitive to 2-amiriopurine and alkylating agents and evidence for the role of DNA methylation in Penicillium chrysogenum (1986)
    Springer
    Current genetics 10 (1986), S. 557-560 
    Details
    ISSN: 1432-0983
    Keywords: Methylation ; DNA ; Penicillium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using high performance liquid chromatography, the presence of N6-methyladenine has been found at a level of 0.1 mol percent in DNA extracted from Penicillium chysogenum. No 5-methylcytosine was detected. A mutant strain HP547, which is sensitive to the lethal effects of N-methyl-N1-nitro-N-nitrosoguanidine, methylmethane sulphonate and the base analogue 2-aminopurine shows an increased spontaneous mutation rate and no detectable DNA methylation. Comparison of restriction enzyme digests of wild type and undermethylated strains indicated that methylation was occurring at a different sequence to that of the Dam methylase system of E. coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447390
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  • 2
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    Isolation of mutants sensitive to 2-amiriopurine and alkylating agents and evidence for the role of DNA methylation in Penicillium chrysogenum (1986)
    Springer
    Details
    Publication Date: 1986-03-01
    Print ISSN: 0172-8083
    Electronic ISSN: 1432-0983
    Topics: Biology
    Published by Springer
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    PAPER CURRENT
  • 3
    Electronic Resource
    Electronic Resource
    Tilt measurements in western Canada (1987)
    Springer
    Pure and applied geophysics 125 (1987), S. 813-834 
    Details
    ISSN: 1420-9136
    Keywords: Tilt ; Western Canada
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Pairs of short baselength mercury-level tiltmeters mounted on shallow piers coupled to sedimentary rocks have been used to monitor ground tilting at three inland locations in western Canada. Noise levels have been estimated over large Nyquist intervals to evaluate site conditions and for comparison with results observed in crystalline rocks at other locations. The results show that tilt noise patterns are similar for different locations, and that noise levels are higher for unconsolidated sediments. Tidal estimates for the principal lunar semidiurnal constituentM 2 were obtained from the least noisy sections of the tilt series. Uncertainties in the estimated amplitudes for the record lengths considered range from 4 to 20% depending on location and azimuth. Comparison of the observedM 2 tide with those predicted for an elastic Earth loaded by two different ocean configurations (Schwiderski or Parke) shows better agreement with the Schwiderski marine tidal model. Differences between observaton and theory suggest a strain-induced perturbation source.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00878034
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  • 4
    Electronic Resource
    Electronic Resource
    Long-term gain changes in mercury-level tiltmeters of the Stacey type (1987)
    Springer
    Pure and applied geophysics 125 (1987), S. 835-843 
    Details
    ISSN: 1420-9136
    Keywords: Tiltmeters ; mercury-level ; gain changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract A uniaxial mercury-level short baselength tilt transducer constructed at the University of Alberta is described. The design closely follows the differential capacitance tiltmeter ofStacey et al. (1969), but differs in that aluminum is used for the beam, and how the mercury cups are supported beneath the beam. It also allows gain verification by comparison of the direct capacitances with a known capacitance. It has been found that the gain factor for the instrument changes with time, because of the relaxation of the mercury surfaces. This has been monitored for several years for four instruments at two sites. The gains change rapidly for several months after installation, but more slowly as time progresses. The magnitudes of such changes in instruments of this type may be as large as 3 percent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00878035
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  • 5
    Electronic Resource
    Electronic Resource
    Extent and nature of extracellular organic production by the marine coccolithophorid Hymenomonas carterae (1989)
    Springer
    Marine biology 100 (1989), S. 381-391 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Production of extracellular dissolved organic substances by cultures of the marine coccolithophorid Hymenomonas carterae SMBA 254 was investigated by the 14C-tracer technique. In 4 h incubations, extracellular 14C production represented 20 to 64% of 14C incorporation into cell material for cultures nearing, or during stationary growth; for rapidly growing cultures with relatively low cell densities the 14C-production was only 4 to 10% of incorporation. Intra-and extra-cellular material was fractionated by ion-exchange membrane electrodialysis. Ionic compounds accounted for 40 to 60% of extracellular material; in contrast only 16 to 27% of intracelular metabolites were in this category. Of a range of methods which were investigated, separation of compounds by gel-filtration in conjunction with two-dimensional thin-layer chromatography proved most effective. Up to 80% of extracellular material was of less than 1 800 mol. wt and included carbohydrates and amino acids, with glutamic acid predominating. The intracellular material was more heterogeneous, with a substantially greater proportion of high molecular weight material. The quite distinct compositions show clearly that extracellular production observed in these experiments arose through excretion and was not attributable to cell lysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391154
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  • 6
    Electronic Resource
    Electronic Resource
    Boundaries of and selectors for upper semi-continuous multi-valued maps (1985)
    Springer
    Mathematische Zeitschrift 189 (1985), S. 297-318 
    Details
    ISSN: 1432-1823
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01164155
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  • 7
    Electronic Resource
    Electronic Resource
    Influence of arginine on the coexistence ofStreptococcus mutans andS. milleri in glucose-limited mixed continuous culture (1987)
    Springer
    Microbial ecology 14 (1987), S. 193-202 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dental plaque is a complex community of bacteria coexisting in an environment frequently limited by carbon and energy sources. UnlikeStreptococcus mutans, other oral streptococci such asS. milleri andS. sanguis have an absolute requirement for and actually consume all available arginine when grown glucose limited in a chemically defined medium. The conditions, particularly in terms of arginine concentration, under which the dental plaque bacteriaS. mutans andS. milleri would coexist under glucose-limiting conditions were investigated. The minimum level of arginine supporting optimal growth ofS. milleri was found to be ca. 50μM, and above this level these strains outcompetedS. mutans. However, coexistence withS. mutans could be achieved at arginine levels of 14–40μM, depending upon theS. milleri andS. mutans strains used. Under such dual limitation,S. milleri was unable to respond to glucose pulses but did respond to pulses of arginine and arginine plus glucose. One of the twoS. milleri strains did not tolerate low pH. In contrast,S. mutans did not tolerate high pH whereasS. milleri was unaffected. This is relevant to dental plaque where arginine catabolism produces a pH rise. Additionally, arginine is an important nutrient since it can be used as an energy source by some oral streptococci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02012940
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  • 8
    Electronic Resource
    Electronic Resource
    Selection pressures on codon usage in the complete genome of bacteriophage T7 (1985)
    Springer
    Journal of molecular evolution 21 (1985), S. 150-160 
    Details
    ISSN: 1432-1432
    Keywords: Bacteriophage T7 ; DNA sequence analysis ; Codon usage ; Molecular evolution ; Synonymous codons ; RNY codons ; Restriction sites ; tRNA ; Pretermination codons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We searched the complete 39,936 base DNA sequence of bacteriophage T7 for nonrandomness that might be attributed to natural selection. Codon usage in the 50 genes of T7 is nonrandom, both over the whole code and among groups of synonymous codons. There is a great excess of purineany base-pyrimidine (RNY) codons. Codon usage varies between genes, but from the pooled data for the whole genome (12,145 codons) certain putative selective constraints can be identified. Codon usage appears to be influenced by host tRNA abundance (particularly in highly expressed genes), tRNA-mRNA interactions (one such interaction being perhaps responsible for maintaining the excess of RNY codons) and a lack of short palindromes. This last constraint is probably due to selection against host restriction enzyme recognition sites; this is the first report of an effect of this kind on codon usage. Selection against susceptibility to mutational damage does not appear to have been involved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02100089
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  • 9
    Electronic Resource
    Electronic Resource
    Qa alloantigen expression on functional T lymphocytes from spleen and thymus (1986)
    Springer
    Immunogenetics 24 (1986), S. 391-401 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The cell-surface expression of the class I alloantigen Qa-2 was analyzed on resting and activated spleen and thymus cells using cytotoxic elimination and immunofluorescence and flow cytometry. Spleen cells activated by mitogens or alloantigen were homogeneously positive for cell surface Qa-2, but activated splenic T cells expressed only about one-third as much Qa-2 per cell as did nonstimulated T cells. These data correlated with the ability to perform cytotoxic elimination with Qa-2-specific monoclonal antibodies (mAbs) in that cytotoxic T lymphocyte (CTL) activity was completely abrogated by pretreatment of spleen cells prior to in vitro culture but was only partially eliminated by treatment of CTL effectors. Qa-2-positive cells constituted only a small subpopulation of fresh normal thymocytes, but were enriched (〉40% positive) among cortisone-resistant thymocytes (CRT). These Qa-2-positive CRT contained mature thymocytes as defined by Ly phenotype Ly-2−, Ly-1hi. When normal thymocytes were treated with Qa-2-specific mAb and complement prior to in vitro sensitization for generation of allogeneic CTL, CTL activity was completely abrogated despite the fact that the fraction of cells eliminated were undetectable as assessed by cell recovery. CTL effectors from alloantigen-stimulated thymocytes were also susceptible to cytotoxic elimination with Qa-2-specific mAb. These data suggest that the Qa-2 molecule may serve not only as a marker on resting and activated peripheral T cells, but also as a unique marker for functionally mature T cells in the thymus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00377958
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  • 10
    Electronic Resource
    Electronic Resource
    Occurrence of a unique MHC class I gene in distantly related members of the genus Mus (1985)
    Springer
    Immunogenetics 21 (1985), S. 267-276 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract There is unequivocal evidence that a relatively nonpolymorphic class I gene (designated Q10) from the Qa region of inbred mice encodes a secreted class 1 molecule. We have used a cDNA probe specific for this gene and an antiserum specific for its secreted protein product to investigate the occurrence and expression of this gene in different species of wild mice broadly representing the entire genus Mus. Evidence is presented that a Q10-like gene has been conserved and is transcribed and translated throughout the genus, suggesting that it serves an important function. However, the data also show that some differences have appeared in this gene over the period of evolutionary time covered by this sampling of wild mice. These results indicate that a specific class I DNA probe isolated from inbred mice can be used to discriminate a particular gene among the multiple class I genes present in other species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00375379
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