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  • 1
    Electronic Resource
    Electronic Resource
    Familial amyloidotic polyneuropathy: transthyretin (prealbumin) variants in kindreds of Italian origin (1988)
    Springer
    Human genetics 〈Berlin〉 80 (1988), S. 341-343 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary As part of an epidemiological study that aims to characterize chemically the mutation(s) in transthyretin (TTR) related to familial amyloidotic polyneuropathy (FAP) of different ethnic origins, studies were carried out on TTR from two FAP kindreds of Italian origin. Two different criteria were employed in the characterization of TTR from these kindreds: (1) immunoblotting of cyanogen bromide fragments for screening of TTR(Met30) and (2) isoelectric focusing. TTR(Met30) was not detected but other substitutions were demonstrated using isoelectric focusing techniques. One of the variants found is a basic TTR variant. The substitutions occurring in the variant TTRs of these two kindreds are not known and are presently under study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273648
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  • 2
    Electronic Resource
    Electronic Resource
    Cell wall composition of the yeast and mycelial forms of Yarrowia lipolytica (1986)
    Springer
    Archives of microbiology 144 (1986), S. 124-130 
    Details
    ISSN: 1432-072X
    Keywords: Dimorphism ; Yarrowia lipolytica ; Cell wall composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cell walls of the yeast and mycelial forms of Yarrowia lipolytica were isolated and purified. Electron microscopy studies showed no differences between both types of cell walls. Chemical analysis revealed that the yeast cell wall contained 70% neutral carbohydrate, 7% amino sugars, 15% protein, 5% lipids and 0.8% phosphorus. Mycelial cell walls contained 70% carbohydrate, 14% aminosugars, 6% protein, 5% lipids and 0.6% phosphorus. Three polysaccharides: β-glucan, mannan and chitin were detected. Proteins were solubilized from both cell wall fractions and separated by polyacrylamide gel electrophoresis. About 50 protein bands were detected, four of them corresponding to glycoproteins. The cell walls of the yeast and mycelial forms of Y. lipolytica were qualitatively similar and only quantitative differences were found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414721
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  • 3
    Electronic Resource
    Electronic Resource
    Replacement of the promoter of the yeast plasma membrane ATPase gene by a galactose-dependent promoter and its physiological consequences (1987)
    Springer
    Current genetics 12 (1987), S. 105-110 
    Details
    ISSN: 1432-0983
    Keywords: Yest ; ATPase ; Galactose promoter ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to probe the physiological role of the yeast plasma membrane ATPase we have replaced the constitutive promoter of its gene by a galactose-dependent promoter. The resulting cells stop growing on glucose medium when the preformed ATPase is diluted to 20% of normal. There is a correlation between ATPase activity and both proton efflux from the cells and amino acid transport. A large proportion of growth-arrested cells appear enlarged and with several buds containing nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00434664
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  • 4
    Electronic Resource
    Electronic Resource
    The effect of substratum on the growth of Terpios, an encrusting sponge which kills corals (1987)
    Springer
    Coral reefs 5 (1987), S. 197-200 
    Details
    ISSN: 1432-0975
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract The effects of substratum on the growth of Terpios was demonstrated using experimental and observational data at Guam, Mariana Islands. Terpios growth was measured on live coral, reef rock, and red calcareous algae in the field. In addition, Terpios was transplanted onto live coral, air-blasted (clean) coral, reef rock, and plexiglass plates, and subsequent growth measured. Terpios grows fastest on clean substrata followed by live coral, reef rock and red calcareous algae in decreasing order. Terpios is sometimes overgrown by Montipora, Porites and red calcareous algae. Since Terpios grows fastest when living coral tissue is removed, it is not likely that Terpios ingests coral tissue as previously suggested in the literature. Instead, Terpios is probably an efficient competitor of corals for space. Terpios overgrows most hard, stable reef substrata, and the growth rate on all sample substrata is substantial. Therefore Terpios has a great potential for covering a reef and may be one of the most important causes of disturbance on some coral reefs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300963
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  • 5
    Electronic Resource
    Electronic Resource
    Room temperature phosphorescence decay of metal chelates in micellar media (1988)
    Springer
    Microchimica acta 96 (1988), S. 269-282 
    Details
    ISSN: 1436-5073
    Keywords: room-temperature phosphorescence ; micelles ; aluminum ; ferron ; metal chelate-triplet state
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The characteristics of the decay of the triplet state of metal chelates have been investigated in micellar media using room temperature phosphorescence (RTP) measurements of the Al-ferron (7-iodo-8-hydroxyquinoline-5-sulfonic acid) complex in cetyltrimethylammonium bromide (CTAB) micelles. Applications of such decay measurements include discrimination between metal complex species and evaluation of the stoichiometry of phosphorescent metal chelates. A thorough study of the effect of foreign ions (interferences) on the photochemical characteristics of RTP of the aluminum complex leads to a tentative classification of foreign cations. Results are compared with those for the Nb(V)-ferron phosphorescent complex in CTAB micelles and possible reaction mechanisms in the presence of micellar media are outlined in an effort to contribute to basic knowledge of micelle stabilized RTP of such inorganic ions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01236111
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  • 6
    Electronic Resource
    Electronic Resource
    A solid-phase enzyme-linked assay for vitamin B12 (1989)
    Springer
    Microchimica acta 97 (1989), S. 65-73 
    Details
    ISSN: 1436-5073
    Keywords: vitamin B12 (cyanocobalamin) ; competitive binding assays ; enzyme-vitamin conjugates ; vitamin tablet analyses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new solid-phase enzyme-linked competitive binding assay for vitamin B12 (cyanocobalamin) is described. The assay is based on the competition between analyte B12 molecules and a glucose-6-phosphate dehydrogenase-vitamin B12 conjugate for a limited number of R-protein binding sites immobilized on sepharose particles. After appropriate incubation and washing steps, the enzyme activity bound to the solid-phase is inversely related to the concentration of B12 in the sample. Under optimized conditions, the method can detect B12 in the range of 3×10−10−1×10−8 M (using 100μl sample) with high selectivity over other biological molecules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01197285
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  • 7
    Electronic Resource
    Electronic Resource
    Molecular defects in cytochrome oxidase in mitochondrial diseases (1988)
    Springer
    Journal of bioenergetics and biomembranes 20 (1988), S. 353-364 
    Details
    ISSN: 1573-6881
    Keywords: Cytochromec oxidase ; respiratory chain ; complex IV ; mitochondrial myopathies ; mitochondrial encephalomyopathies ; maternal inheritance ; isozymes ; Leigh syndrome ; MERRF (myoclonus epilepsy with ragged-red fibers)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Defects of cytochromec oxidase (COX) show remarkable clinical, biochemical, and genetic heterogeneity. Clinically, there are two main groups of disorders, one dominated by muscle involvement, the other by brain dysfunction. Biochemically, the enzyme defect may be confined to one or a few tissues (reflecting the existence of tissue-specific isozymes) or affect all tissues. Immunologically reactive enzyme protein is decreased in some forms of COX deficiency but not in others. Because COX is encoded both by nuclear and by mitochondrial genes, COX deficiencies may be due to mutations of either genome and may offer useful models to study the communication between nuclei and mitochondria. We have isolated full-length cDNA clones encoding human COX subunits IV, Vb, and VIII and a partial-length clone for subunit Va. These clones are being used as probes to analyze the DNA and RNA of patients with COX deficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00769637
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  • 8
    Electronic Resource
    Electronic Resource
    Calorimetric measurement of ionexchange heats of homoionic heulandite and mordenite (1987)
    Springer
    Journal of thermal analysis and calorimetry 32 (1987), S. 949-951 
    Details
    ISSN: 1572-8943
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The ionexchange heats of homoionic heulandite and mordenite were measured with a microcalorimeter. In the case of K, NH4, Na, Mg and Ca, the selectivity of the zeolite is determined by the heat of hydration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01913782
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  • 9
    Electronic Resource
    Electronic Resource
    An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in leaf crude extracts (1987)
    Springer
    Photosynthesis research 14 (1987), S. 269-278 
    Details
    ISSN: 1573-5079
    Keywords: ELISA ; fructose-1,6-bisphosphatase ; photosynthesis ; quantitative determination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in crude extracts of leaves is proposed. It is based on the ELISA technique, and offers two modifications. A non-competitive technique has a higher sensitivity and is the right option for samples of low fructose-1,6-bisphosphatase content. However, this method is not sufficiently specific when the total protein is higher than 5 μg/cm3; so, despite its lower sensitivity, in these circumstances a competitive technique is more suitable. Thus photosynthetic fructose-1,6-bisphosphatase can be measured without interferences from the gluconeogenic cytosolic enzyme of the photosynthetic cell or from a non-specific phosphatase present in the chloroplast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00032710
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  • 10
    Electronic Resource
    Electronic Resource
    Ciliates in a waste stabilization pond system in Mexico (1987)
    Springer
    Water, air & soil pollution 34 (1987), S. 245-262 
    Details
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A protozoological survey was performed on a waste-stabilization pond to isolate, identify, and determine the incidence of ciliates present in these waters. The correlation between the species found and the physicochemical parameters was investigated. One hundred and eighty samples were analyzed from February 1983 to February 1984. Free-living ciliates isolated include:Carchesium polypinum Linnaeus,Vorticella microstoma Ehrenberg,Vorticella convallaria Linnaeus,Didinium balbiani Fabré-Domergue,Amphileptus claparedei Stein,Spathidium spathula Müller,Litonotus fasciola Ehrenberg,Paramecium caudatum Müller,Paramecium aurelia Ehrenberg,Tetrahymena vorax Kidder,Tetrahymena pyriformis Ehrenberg,Cyclidium glaucoma Müller,Aspidisca costata Ehrenberg, andStylonychia putrina Stokes. The performance of the pond was predominantly anaerobic. The temperature, total alkalinity, and nitrate concentration were the most variable parameters in the system studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193773
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