Publication Date:
1989-06-23
Description:
Guanosine 5'-triphosphate (GTP)-binding proteins have been implicated in the transport of newly synthesized proteins along the secretory pathway of yeast and mammalian cells. Early vesicle fusion events that follow receptor-mediated endocytosis as measured by three in vitro assays were blocked by guanosine 5'-O-(3-thiotriphosphate) and aluminum fluoride. The effect was specific for guanosine nucleotides and depended on the presence of cytosolic factors. Thus, GTP-binding proteins may also have a role in the transport of molecules along the endocytic pathway.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mayorga, L S -- Diaz, R -- Stahl, P D -- AI 20015/AI/NIAID NIH HHS/ -- CA 12858/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1989 Jun 23;244(4911):1475-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2499930" target="_blank"〉PubMed〈/a〉
Keywords:
Biological Transport/drug effects
;
Cell Line
;
Cell-Free System
;
Cytosol/physiology
;
Dinitrophenols/immunology/metabolism
;
*Endocytosis/drug effects
;
Exocytosis
;
GTP-Binding Proteins/*physiology
;
Glucuronidase/metabolism
;
Guanosine 5'-O-(3-Thiotriphosphate)
;
Guanosine Triphosphate/analogs & derivatives/pharmacology
;
Immunoglobulin G/metabolism
;
Immunosorbent Techniques
;
Intracellular Membranes/physiology
;
Macrophages/metabolism/ultrastructure
;
Membrane Fusion/drug effects
;
Organelles/ultrastructure
;
Thionucleotides/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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