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  • 1
    Electronic Resource
    Electronic Resource
    Evolutionary conservation of the 3′ ends of members of a family of giant secretory protein genes inChironomus pallidivittatus (1987)
    Springer
    Journal of molecular evolution 25 (1987), S. 20-28 
    Details
    ISSN: 1432-1432
    Keywords: Balbiani rings ; Evolution ; Immunological detection ; Nucleotide sequence ; 3′ End
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two cDNA clones representing the 3′-end regions of BR1 and BR2 75S mRNA were obtained fromChironomus pallidivittatus. The regular structure characterizing the core of these genes, consisting of tandemly arranged repeat units, changes into a more irregular structure toward the 3′ end. Distal to a standard type of repeat unit with a characteristic excess of positive charges, a new type of repeat with a high, negative charge density is interspersed among parts of the standard unit. The last 111 amino acids before the stop codon represent a unique region distinctly different in amino acid composition from upstream regions, and include two partially homologous hydrophobic regions. Sequence comparison of 3′-end regions from clones representing BR1 and BR2 genes indicates striking sequence conservation in the unique part of the region. Analysis of the level of silent site divergence shows that the homology increases in the 3′ direction up to the polyadenylation site. That the unique region is retained as a part of the secreted protein is shown by Western blotting.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02100037
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  • 2
    Electronic Resource
    Electronic Resource
    Cotransposition of a highly repetitive DNA element with flanking sequences in the genome of the midgeChironomus thummi (1987)
    Springer
    Journal of molecular evolution 26 (1987), S. 311-319 
    Details
    ISSN: 1432-1432
    Keywords: Chironomus thummi ; Repetitive DNA ; Cotransposition ; NTS of rDNA ; Claelements ; Satellite DNA ; DNA amplification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A family of highly repetitive DNA elements, the Cla-elements, is present in the genomes of the two sibling speciesChironomus th. thummi andCh. th. piger. These Cla-elements are organized in large tandem repetitive clusters as well as occuring as interspersed monomeric elements, in both subspecies. The analysis of a monomeric Cla-element and several kilobases of its flanking sequences fromCh. th. piger revealed that the short Cla-elements are cotransposed together with adjacent DNA. We found the same association of Cla-elements with specific flanking DNA in clones obtained from the rDNA ofCh. th. thummi and from nonribosomal Cla-DNA ofCh. th. piger. The Cla-element-flanking DNA is clearly also repetitive, but mainly of inter-spersed organization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02101149
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  • 3
    Electronic Resource
    Electronic Resource
    Zur formalen Genetik der Phosphoglucomutasen (EC: 2.7.5.1); Untersuchung von 366 Familien (1969)
    Springer
    Human genetics 〈Berlin〉 8 (1969), S. 67-68 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The polymorphism of PGM1 has been investigated in a series of 366 families from Southwestern Germany with 676 children. The segregation of the children's phenotypes is in agreement with the formal genetic model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286759
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  • 4
    Electronic Resource
    Electronic Resource
    The use of 8-hydroxyquinoline for the isolation and prequalification of plant growth-stimulating rhizosphere pseudomonads (1985)
    Springer
    Biology and fertility of soils 1 (1985), S. 167-173 
    Details
    ISSN: 1432-0789
    Keywords: Iron chelation ; Siderophores ; Selective medium ; Bacterization ; Raphanus sativus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The application of 8-hydroxyquinoline (8OHQ) in a low-iron medium is demonstrated to be an important selective agent providing a simple and non-laborious method in the search for plant growth-stimulating and antagonistic fluorescent pseudomonads. Eleven isolates obtained from low-iron plates containing 120 ppm 8OHQ possessed similar or even better antagonistic properties in vitro than the reference strains Pseudomonas fluorescens WCS 374 and pseudobactin-producing strain B10 with plant growth-stimulating properties. One out of four of the best in vitro antagonists showed significant growth stimulation of radish (Raphanus sativus), comparable to that of the plant growth-stimulating reference strains. Iron supply abolished their antagonism in vitro, indicating that siderophores were primarily involved. The selective mode of action of 8OHQ was demonstrated to be iron stress induced by the strong chelation of available iron.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257633
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  • 5
    Electronic Resource
    Electronic Resource
    Occurrence and nature of mixed infections in nodules of field-grown soybeans (Glycine max) (1987)
    Springer
    Biology and fertility of soils 5 (1987), S. 112-114 
    Details
    ISSN: 1432-0789
    Keywords: Mixed nodule infections ; Glycine max ; Soybeans ; Bradyrhizobium japonicum strains ; Field inoculation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Mixed infections of Bradyrhizobium japonicum strains in early and late nodules of four soybean cultivars were studied in a field soil. Nodule occupants were identified by immunofluorescence using serogroup specific antibodies prepared against B. japonicum strains USDA 110, USDA 123, and USDA 138. Double infection was determined directly by combined examination of the same microscopic field by fluorescence and phase contrast microscopy. Double strain occupancy was observed consistently, and its occurrence did not differ substantially in pouch, soil pot, and field experiments, ranging in incidence from 12% to 32%. No significant differences in the incidence or nature of double infection could be attributed to cultivar, seed inoculation, or plant maturity. Strains reactive to strain USDA 123-fluorescent antibody were dominant in both singly and doubly infected nodules irrespective of cultivar, plant age, or seed inoculation with strain USDA 110.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257644
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  • 6
    Electronic Resource
    Electronic Resource
    Competitiveness and persistence of strains ofRhizobium phaseoli introduced into a Moroccan sandy soil (1989)
    Springer
    Biology and fertility of soils 7 (1989), S. 213-218 
    Details
    ISSN: 1432-0789
    Keywords: Rhizobium phaseoli ; Competition ; Persistence ; Strain-cultivar interaction ; Cultivar interaction ; Growth chamber
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sixteen strains ofRhizobium phaseoli were isolated from the Loukkos sandy soil and were compared with four selected strains ofR. phaseoli (CIAT 676, CIAT 57, Olivia, Viking 1) for their N2-fixing ability and for their serological affinities by means of the fluorescent antibody technique. Two indigenous strains were rated as highly effective, six as moderately effective, and eight were ineffective. Viking 1 was ranked as highly effective while the other inoculum strains were moderately effective. None of the 4 inoculum strains cross-reacted with the 16 indigenous strains. The indigenous strains were grouped into only two serogroups but showed a high degree of heterogeneity in regard to the strength of the immunofluorescence response. The inoculum strains outcompeted but did not eliminate the resident population for nodule occupancy on two French bean cultivars (Royalnel and Fetiche). The competitiveness, however, differed between inoculum strains. It was influenced by the host cultivar and by the host cultivar growth stage. Viking 1 was consistent in its high competitive ability whether it was inoculated singly or in a mixture with the other strains. It showed high persistence and formed more than 50% of the nodules 1 year after it was introduced. CIAT 57, Olivia, and CIAT 676 were mediocre in their persistence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00709651
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  • 7
    Electronic Resource
    Electronic Resource
    Pharmacokinetics of triamcinolone acetonide and its phosphate ester (1985)
    Springer
    European journal of clinical pharmacology 29 (1985), S. 85-89 
    Details
    ISSN: 1432-1041
    Keywords: triamcinolone acetonide ; triamcinolone acetonide phosphate ; pharmacokinetics ; high dose ; glucocorticoids ; renal excretion ; metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Triamcinolone acetonide in the form of its phosphate ester was given intravenously in two different doses (10 mg/kg and 80 mg). Plasma levels of the ester and triamcinolone acetonide were measured and pharmacokinetic parameters were calculated. The pharmacokinetics both of the phosphate and the free alcohol were dose-dependent. No unchanged ester was found in the urine, indicating complete conversion of the pro-drug. Triamcinolone was not a major metabolite of triamcinolone acetonide in humans. Renal clearance was low and independent of the dose. Only about 1% of the dose was found in the urine as triamcinolone acetonide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00547374
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  • 8
    Electronic Resource
    Electronic Resource
    Oral Immunization of Mice with a Soluble Protective Antigen obtained from Enteropathogenic Serotypes of Escherichia coli (1967)
    [s.l.] : Nature Publishing Group
    Nature 214 (1967), S. 1053-1054 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The extracts produced in this way were examined serologically by the latex test, using several specific anti-E. coli sera. The extracts proved to be group-specific, that is, they only gave reactions with the E. coli antisera which contained antibodies against enteropathogenic E. coli type 0.111. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2141053a0
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  • 9
    Electronic Resource
    Electronic Resource
    Release of Enzymes from the Liver (1967)
    [s.l.] : Nature Publishing Group
    Nature 213 (1967), S. 1125-1126 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We studied the release of cellular enzymes in isolated perfused rat liver. By isolating the organ we were able to avoid the influence of extrahepatic factors and the superposition of enzyme patterns from various tissues. The rat livers were perfused by the method of Miller1 as modified by ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2131125a0
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  • 10
    Electronic Resource
    Electronic Resource
    Multiple Steady States in a Coupled Enzyme System Represented by the Enzymatically Catalyzed Production of l-Phenylalanine (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 501 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb45750.x
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