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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 141 (1994), S. 277-287 
    ISSN: 1432-1424
    Keywords: Erythrocytes (red blood cells) ; Amiloride ; Magnesium ; sodium exchange ; Magnesium transport ; Sodium transport ; Hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Previous work showed that in hamster red cells the amiloride-sensitive (AS) Na+ influx of 0.8 mmol/liter cells/hr is not mediated by Na-H exchange as in other red cells, but depends upon intracellular Mg2+ and can be increased by 40-fold by loading cells with Mg2+ to 10 mm. The purpose of this study was to verify the connection of AS Na+ influx with Na-dependent, amiloride-sensitive Mg2+ efflux and to utilize AS Na+ influx to explore that pathway. Determination of unidirectional influx of Na+ and net loss of Mg2+ in parallel sets of cells showed that activation by extracellular [Na+] follows a simple Michaelis-Menten relationship for both processes with a K m of 105–107 mm and that activation of both processes is sigmoidally dependent upon cytoplasmic [Mg2+] with a [Mg2+]0.5 of 2.1–2.3 mm and a Hill coefficient of 1.8. Comparison of Vmax for both sets of experiments indicated a stoichiometry of 2 Na: l Mg. Amiloride inhibits Na+ influx and Mg2+ extrusion in parallel (K i = 0.3 mm). Like Mg2+ extrusion, amiloride-sensitive Na+ influx shows an absolute requirement for cytoplasmic ATP and is increased by cell swelling. Hence, amiloride-sensitive Na+ influx in hamster red cells appears to be through the Na-Mg exchange pathway. There was no amiloride-sensitive Na+ efflux in hamster red cells loaded with Na+ and incubated with high [Mg2+] in the medium with or without external Na+, nor with ATP depletion. Hence, this is not a simple Na-Mg exchange carrier.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 159 (1993), S. 392-395 
    ISSN: 1432-072X
    Keywords: Arthrobacter strain 45-8A ; Cephalosporin acylase ; 7-Aminocephalosporanic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A bacterial strain producing cephalosporin acylases was isolated from soil. The morphological and physiological properties of this strain suggest that it belongs to the genus Arthrobacter, and the isolate was therefore designated Arthrobacter strain 45-8A. Substrate specificity of the enzyme was examined. The enzyme can convert both cephalosporin C and 7β-(4-carboxylbutan-amino)cephalosporanic acid to 7-aminocephalosporanic acid. An interesting feature of the acylases is their temperature-dependent regulation. Activity of acylases was detected in strain 45-8A grown at temperature below 30 °C, but was not observed at higher temperature. Arthrobacter strain 45-8A did not exhibit β-lactamase activity, even though its resistance to cephalosporin C was very strong (〉2000 μg/ml). This is quite beneficial for its application in the manufacture of 7-aminocephalosporanic acd.
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  • 3
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 75 (1994), S. 2286-2288 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Using a high Tc superconducting grain boundary Josephson junction, harmonic mixing experiments in the mm waveband were carried out, aiming at as large a harmonic number and as high a signal frequency as possible. The dependencies of intermediate frequency output on dc bias, harmonic number, frequency of local oscillator (LO), and other parameters were carefully studied. Until now, our best result was the mixing between the signal at 95 GHz and the 105th harmonic of LO at about 900 MHz. Preliminary experiments using a high Tc harmonic mixer and phase-locking loop were tried to stabilize the frequency of a mm wave source.
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Industrial & engineering chemistry research 32 (1993), S. 2614-2619 
    ISSN: 1520-5045
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Two crystal structures of the glucocorticoid receptor DNA-binding domain complexed with DNA are reported. The domain has a globular fold which contains two Zn-nucleated substructures of distinct conformation and function. When it binds DNA, the domain dimerizes, placing the subunits in adjacent ...
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 88 (1994), S. 685-690 
    ISSN: 1432-2242
    Keywords: Festuca arundinacea ; Lolium perenne ; Genome-specific probes ; Grass breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The wild species of tall fescue (Festuca arundinacea var.genuina Schreb.) represent a wide range of genetic variation and constitute potential germplasm for tall fescue improvement. Our objective was to evaluate genome specificity of the previously-identified DNA probes and to examine the phylogenetic relationship of tall fescue with six related species by using RFLP data. A total of 29 DNA probes from aPstI-genomic library of tall fescue were hybridized toEcoRI-orHindIII-digested DNA of 32 plants from sixFestuca species and fromLolium perenne L. Fifteen probes hybridized to all seven species. The remaining 14 probes showed differential hybridization patterns (i.e., ±), especially at the diploid and tetraploid levels. This hybridization pattern reflected genome divergence in these species. The DNA probes will be useful markers in breeding programs involving interspecific and intergeneric hybridization. Cluster analyses were performed using the average genetic distances calculated with the RFLP data from 53 probe-enzyme combinations. Generally, genotypes from the same species were grouped in the same cluster. These data indicated that tall fescue has a close relationship withF. pratensis Huds. (diploid),F. arundinacea var.glaucescens Boiss. (tetraploid), andL. perenne L. (diploid) and thatFestuca pratensis andL. perenne had the closest degree of relationship.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Reaction kinetics and catalysis letters 42 (1990), S. 47-53 
    ISSN: 1588-2837
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Abstract Промотирование катализатора K/Fe/силикалит-2 лантаном повышает селективность олефинов C2–C4 и конверсию моноокиси углерода. Влияние лантана на катализатор изучали с помощью ТПВ, ТПД и РФЭС методов. Определяли оптимальное содержание лантана в катализаторе.
    Notes: Abstract The promotion of a K/Fe/silicalite-2 catalyst with lanthanum increases the selectivity of C2–C4 olefin and conversion of carbon monoxide. The influence of lanthanum on the catalyst has been characterized by TPR, TPD and XPS techniques. The suitable lanthanum content in the chemical composition of the catalyst is determined.
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  • 8
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Mössbauer and magnetic measurements on Cr75(Fe,Mn)25 alloys, which were segregated and re-disordered by appropriate heat treatments, are reported. A primary factor for the kind of magnetic order which appears is the Cr concentration around the Mössbauer probe atom. At around 15 K, a transition to a re-entrant spin-glass state occurs. For this state, the hyperfine field distributions of all investigated disordered and re-disordered samples are independent of the Fe concentration.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Experimental mechanics 32 (1992), S. 83-88 
    ISSN: 1741-2765
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The grating objective speckle method has been applied in whole-field strain analysis of carbon-fiber-woven polyimide-composite materials. A sequence of rosette fringe patterns was obtained from one single specklegram, indicating displacement components along four different directions with a 45-deg interval. The spatial frequencies, which represent the sensitivities of the fringe intervals, were 2400 ℓ/mm forU x ,U y and 1697 ℓ/mm forU 45 andU 135. Normal strain components, ɛ x , ɛ y , ɛ45, and ɛ135, were trasformed to quantitative topographic representations by coupling to a digital computer system. Shear strain can be determined by the rosette equations.
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  • 10
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