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Characterization of genetic identities and relationships of Brassica oleracea L. via a random amplified polymorphic DNA assay (1992)

Kresovich, S. ; Williams, J. G. K. ; McFerson, J. R. ; [et al.]

Springer

Theoretical and applied genetics 85 (1992), S. 190-196

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ISSN: 1432-2242

Keywords: DNA typing ; Genetic similarity ; Genetic structure ; Genetic resource conservation ; Vegetable and forage cole crops

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Effective conservation and the use of plant genetic resources are essential for future agricultural progress. Critical to this conservation effort is the development of genetic markers which not only distinguish individuals and accessions but also reflect the inherent variation and genetic relationships among collection holdings. We have examined the applicability of the random amplified polymorphic DNA (RAPD) assay for quick, cost-effective, and reliable use in addressing these needs in relation to collection organization and management. Twenty-five decamer oligonucleotide primers were screened individually with a test array composed of individuals representing a range of genetic relationships in Brassica oleracea L. (vegetable and forage cole crops). Over 140 reproducible, polymorphic fragments were generated for study. Each individual of the test array exhibited a unique molecular genotype and composites specific for accessions and botanical varieties could be established. An analysis of similarity based on amplified DNA fragments reflected the known genetic relationships among the selected entries. These results demonstrated that RAPD markers can be of great value in gene bank management for purposes of identification, measurement of variation, and establishment of genetic similarity at the intraspecific level.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00222859

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Genetic diversity and taxonomy of Portuguese Tronchuda cabbage and Galega kale landraces using isozyme analysis (1994)

Dias, J. S. ; Monteiro, A. A. ; Kresovich, S.

Springer

Euphytica 75 (1994), S. 221-230

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ISSN: 1573-5060

Keywords: allozyme variation ; allozyme polymorphism ; Brassica oleracea ; classification ; Portuguese coles

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Summary Isozyme analysis was used to study the genetic variation and the genetic relationships of a collection of 48 Brassica spp. including 31 Portuguese coles (Brassica oleracea L.) accessions representative of the different landraces cultivated in Portugal. Other brassicas included in this experiment were Jersey kale, kailaan, common cabbages, broccolis, cauliflower, nine-chromosome wild brassica and turnip. Nine enzymes used in the starch gel electrophoresis included: PGM, PGI, AAT, LAP, TPI, FBP, SOD, IDH and GR. Twenty-one putative loci were revealed, with 3 showing invariance and the other 18 contained 50 alleles. The allelic frequencies at these loci represented by 40 plants per accession were used to calculate the following estimators of genetic variation: % of polymorphic loci, average number of alleles per polymorphic loci, average number of alleles per locus, and index of heterozygosity. The genetic relationships were evaluated considering Nei (1978) and Rogers (1972) genetic distances between each pair of accessions whose matrices were hierarchically clustered by the UPGMA method. The accessions were also studied using the principal coordinate analysis. Portuguese Tronchuda cabbages and Galega kales have shown high genetic diversity in comparison with the other accessions. This indicates their potential variation for use in breeding programs. The UPGMA results show that the 48 accessions, with the exception of B. insularis, B. cretica, and turnip, can be clustered into 6 groups: (a) Portuguese Tronchuda cabbages, Galega kales and Algarve cabbages; (b) common cabbages and kales; (c) Couve Poda do Algarve and broccoli; (d) Algarve cabbage and common cabbages; (e) kailaan; (f) broccoli and cauliflower. The groupings obtained by the isozyme analysis are difficult to interpret considering the origin of the Brassica spp. and the morphological resemblance among the accessions.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00025607

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