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Phenol degradation by a psychrotrophic strain of Pseudomonas putida (1991)

Kotturi, Gopaul ; Robinson, Campbell W. ; Inniss, William E.

Springer

Applied microbiology and biotechnology 34 (1991), S. 539-543

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ISSN: 1432-0614

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Summary Cell growth and phenol degradation kinetics were studied at 10°C for a psychrotrophic bacterium, Pseudomonas putida Q5. The batch studies were conducted for initial phenol concentrations, So, ranging from 14 to 1000 mg/1. The experimental data for 14〈=So〈=200 mg/1 were fitted by non-linear regression to the integrated Haldane substrate inhibition growth rate model. The values of the kinetic parameters were found to be: μm=0.119 h−1, K S=5.27 mg/1 and K I=377 mg/1. The yield factor of dry biomass from substrate consumed was Y=0.55. Compared to mesophilic pseudomonads previously studied, the psychrotrophic strain grows on and degrades phenol at rates that are ca. 65–80% lower. However, use of the psychrotrophic microorganism may still be economically advantageous for waste-water treatment processes installed in cold climatic regions, and in cases where influent waste-water temperatures exhibit seasonal variation in the range 10–30°C.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00180585

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Enhanced disruption of Candida utilis using enzymatic pretreatment and high-pressure homogenization (1994)

Baldwin, Clark V. ; Robinson, Campbell W.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 43 (1994), S. 46-56

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ISSN: 0006-3592

Keywords: enzymatic lysis ; homogenization, high-pressure ; Candida utilis ; cell wall disruption ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: The enhancement of the overall disruption of a native strain of Candida utilis (ATCC 9226) was studied using a combination of two methods, namely, pretreatment in the form of partial enzymatic lysis by Zymolyase followed by mechanical disruption in a Microfluidizer high-pressure homogenizer. The cells were grown in both batch and continuous cultures to examine the effect of specific growth rate on disruption. Cell suspensions ranging in concentration from 7 to 120 g DW/L were disrupted with and without enzymatic pretreatment. For yeast grown in batch culture, final total disruption obtained using the combined protocol approached 95% with four passes at a pressure of 95 MPa, as compared with only 65% disruption using only mechanical homogenization. A modified model was developed to predict the fraction disrupted by the enzymatic pretreatment-mechanical homogenization two-stage process. Predicted disruptions agreed favorably with experimental observations (maximum deviation of 20%) over a wide range of operating conditions. © 1994 John Wiley & Sons, Inc.

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260430107

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Comments on the communication: On the calculation of shear rate and apparent viscosity in airlift and bubble column bioreactors (1991)

Allen, D. Grant ; Robinson, Campbell W.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 38 (1991), S. 212-216

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ISSN: 0006-3592

Keywords: airlift bioreactor ; shera rates in reactors ; hydrodynamics ; non-Newtonian fluids ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: The determination of the shear rate in bubble column and airlift bioreactors is an important question from both the perspective of cell damage and the correlation of hydrodynamic parameters in non-Newtonian fluids in these contractors. In the context of correlating hydrodynamic parameters in non-Newtonian fluids, a common approach involves assuming that there exists an average shear rate in the column that is proportional to the superficial gas velocity. This average shear rate is then used to evaluate an effective viscosity of the non-Newtonian fluid that is subsequently used to quantify the fluid's rheological behavior in correlation. Contrary to a recent communication, this report illustrates that this approach, which has mainly been applied to bubble columns, can also be applied to external loop airlift contractors, replacing the superficial gas velocity by the superficial gas velocity by the superficial gas velocity supplied to the riser of the contractor. This extension is based upon consideration of the relevant characteristic velocity in the active zone (i.e., the riser section) of the reactor.

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260380214

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