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  • 1
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 76 (1994), S. 8113-8116 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Diamond and amorphous carbon films have been deposited on silicon (100) substrates by filament-assisted XeCl excimer laser ablation of graphite targets. The influence of process parameters on the growth mechanism of diamond were studied by depositing films over a wide range of gas pressures and substrate temperatures. The surface morphology and bonding of the deposited films were characterized by scanning electron microscopy, and Raman and electron-energy-loss spectroscopy. In these experiments, microcrystalline diamond films with growth rates comparable to conventional chemical-vapor-deposition and plasma-enhanced chemical-vapor-deposition techniques could only be obtained at substrate temperatures and hydrogen pressures greater than 700 °C and 1.3 mbar, respectively. Conversely, the best conditions for growing carbon films with predominately sp3-type bonding structures were at temperatures and pressures less than 300 °C and 0.3 mbar, respectively. These results suggest that carbon-hydrogen gas phase reactions as well as gas-surface reactions are both necessary for the formation of diamond. © 1994 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A multicopy plasmid containing the Escherichia coli fur gene was introduced into Pseudomonas aeruginosa strain PA103C. This strain contains a toxA-lacZ fusion integrated into its chromosome at the toxA locus. Beta-galactosidase synthesis in this strain is regulated by iron, as is seen for exotoxin A production. Beta-galactosidase synthesis and exotoxin A production in PA103C containing multiple copies of E. coli fur was still repressed in low iron conditions. The transcription of regA, a positive regulator of toxA, was also found to be inhibited by multiple copies of the E. coli fur gene. In addition, the ability of PA103C containing multiple copies of E. coli fur to produce protease was greatly reduced relative to PA103C containing a vector control.A polyclonal rabbit serum containing antibodies that recognize E. coli Fur was used to screen whole-cell extracts from Vibrio cholerae, Shigella flexneri, Salmonella typhimurium and Pseudomonas aeruginosa. All strains tested expressed a protein that was specifically recognized by the anti-Fur serum. These results and those described above suggest that Fur structure and function are conserved in a variety of distinct bacterial genera and that at least some of these different genera use this regulatory protein to control genes encoding virulence factors.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1572-9729
    Keywords: analytical chemistry ; bioassay ; biochemistry ; composition ; hydrocarbons ; remediation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Bioremediation of petroleum-contaminated sites is expected to be a cost-effective remediation technology. However, many potential users of the technology expect the reliability of this technology to be similar to other candidate technologies for widespread consideration. In particular, candidate technologies should possess the property of reliable experimental linkage — there should be reasonable confidence that experiments done at one scale can be reliably related to another. An important example is bench-scale treatability studies that should result in linkages with commercial-scale operations. In this respect comparison of bioremediation to other candidate technologies reveals that bioremediation is in an early stage of its evolution. It is being pursued at a variety of sites and scales with practitioners from a variety of disciplines. Integration of activities between disciplines and an ability to quantitatively compare results at different sites and scales is proceeding. This paper addresses a number of physical, chemical, biological, analytical, and statistical issues regarding the successful comparison of results between experiments.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 1994-12-15
    Print ISSN: 0021-8979
    Electronic ISSN: 1089-7550
    Topics: Physics
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  • 5
  • 6
    Publication Date: 1994-01-01
    Print ISSN: 0923-9820
    Electronic ISSN: 1572-9729
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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  • 7
    Publication Date: 2011-08-24
    Description: A system was developed in which nutrient flow to plant roots is controlled by a thin (0.98 or 1.18 mm) porous (0.2 or 0.5 microns) stainless steel sheet membrane. The flow of nutrient solution through the membrane is controlled by adjusting the relative negative pressure on the nutrient solution side of the membrane. Thus, the nutrient solution is contained by the membrane and cannot escape from the compartment even under microgravity conditions if the appropriate pressure gradient across the membrane is maintained. Plant roots grow directly on the top surface of the membrane and pull the nutrient solution through this membrane interface. The volume of nutrient solution required by this system for plant growth is relatively small, since the plenum, which contains the nutrient solution in contact with the membrane, needs only to be of sufficient size to provide for uniform flow to all parts of the membrane. Solution not passing through the membrane to the root zone is recirculated through a reservoir where pH and nutrient levels are controlled. The size of the solution reservoir depends on the sophistication of the replenishment system. The roots on the surface of the membrane are covered with a polyethylene film (white on top, black on bottom) to maintain a high relative humidity and also limit light to prevent algal growth. Seeds are sown directly on the stainless steel membrane under the holes in the polyethylene film that allow a pathway for the shoots.
    Keywords: Man/System Technology and Life Support
    Type: HortScience : a publication of the American Society for Horticultural Science (ISSN 0018-5345); Volume 25; 6; 707
    Format: text
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  • 8
    Publication Date: 2013-08-31
    Description: The requirements for life support systems, both biological and physical-chemical, for long-term human attended space missions are under serious study throughout NASA. The KSC 'breadboard' project has focused on biomass production using higher plants for atmospheric regeneration and food production in a special biomass production chamber. This chamber is designed to provide information on food crop growth rate, contaminants in the chamber that alter plant growth requirements for atmospheric regeneration, carbon dioxide consumption, oxygen production, and water utilization. The shape and size, mass, and energy requirements in relation to the overall integrity of the biomass production chamber are under constant study.
    Keywords: MAN/SYSTEM TECHNOLOGY AND LIFE SUPPORT
    Type: NASA. Johnson Space Center, The Second Conference on Lunar Bases and Space Activities of the 21st Century, Volume 2; p 537-542
    Format: application/pdf
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  • 9
    Publication Date: 2019-06-28
    Description: The Biomass Production Chamber at Kennedy Space Center was constructed to conduct large scale plant growth studies for NASA's CELSS program. Over the past four years, physical systems and computer control software have been continually upgraded and the degree of atmospheric leakage from the chamber has decreased from about 40 to 5 percent of the total volume per day. Early tests conducted with a limited degree of closure showed that total crop (wheat) growth from the best trays was within 80 percent of reported optimal yields for similar light levels. Yields from subsequent tests under more tightly closed conditions have not been as good--up to only 65 percent of optimal yields. Yields appear to have decreased with increasing closure, yet potential problems exist in cultural techniques and further studies are warranted. With the ability to tightly seal the chamber, quantitative data were gathered on CO2 and water exchange rates. Results showed that stand photosynthesis and transpiration reached a peak near 25 days after planting, soon after full vegetative ground cover was established. In the final phase of testing when atmospheric closure was the highest, ethylene gas levels in the chamber rose from about 10 to nearly 120 ppb. Evidence suggests that the ethylene originated from the wheat plants themselves and may have caused an epinastic rolling of the leaves, but no apparent detrimental effects on whole plant function.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-TM-103494 , NAS 1.15:103494
    Format: application/pdf
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