ISSN:
0941-1216
Keywords:
Chemistry
;
Organic Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Selectivity: OH-, NH- and SH-Groupspecific Reagents. The Application in Organic Analysis and as Protective GroupsPhosphylhalides and -pseudohalides R1R2P(O)X (X = Cl, F, CN, N3, OC6H4NO2(p)) reactdepending on X- with a different selectivity with OHNH- and SH-groups. Vinylsulfones ArSO2CH = CH2 are SH-selective. Silanes R3SiH are OH-selective.By exchange of one ligand bound on P, SO2 or Si by the 5-dimethylamino resp. the 5-methoxynaphthalinogroup reagents are formed, which combine group selectivity with fluorescence. The fluorescence is quenched if a ligand or the leaving group is substituted by a NO2-group.The fluorescence appears again if after the group selective fixation the ligand with the nitrogroup is split off.In serine enzymes (α-chymotrypsine, trypsin, butyryl- and acetylcholinesterase, subtilisine) and insuline (after the reductive opening of the S-S bridges) the primary OH-groups in the active site in the enzymes resp. the SH groups in the reduced insuline were substituted with fluorescing groups using group selective fluorescing reagents.
Additional Material:
8 Tab.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/prac.19923340802
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