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  • 1990-1994  (5)
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Year
  • 1
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    Unknown
    Application of RNA/DNA ratio and tryptic enzyme activity on laboratory-reared and wild-caught herring larvae (1993)
    VCH Verlagsgesellschaft mbH
    In:  In: Fish Ecotoxicology and Ecophysiology. , ed. by Braunbeck, T., Hanke, W. and Segner, H. VCH Verlagsgesellschaft mbH, Weinheim, pp. 227-232.
    Details
    Publication Date: 2018-03-05
    Type: Book chapter , PeerReviewed
    Format: text
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  • 2
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    Unknown
    The effect of food availability, age or size on the RNA/DNA ratio of individually measured herring larvae: laboratory calibration (1994)
    Springer
    In:  Marine Biology, 118 . pp. 377-382.
    Details
    Publication Date: 2018-06-06
    Description: RNA/DNA ratios in individual herring (Clupea harengus) larvae (collected from Kiel Bay, Baltic Sea, in 1989) were measured and proved suitable for determining nutritional status. Significant differences between fed and starving larvae appeared after 3 to 4 d of food deprivation in larvae older than 10 d after hatching. The RNA/DNA ratio showed an increase with age or length of the larvae and was less pronounced in starving larvae compared to fed larvae. The individual variability of RNA/DNA ratios in relation to larval length of fed larvae and of larvae deprived of food for intervals of 6 to 9 d is presented. Based on the length dependency and the individual variability found within the RNA/DNA ratios, a laboratory calibration is given to determine whether a larva caught in the field has been starving or not. An example for a field application is shown.
    Type: Article , PeerReviewed
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  • 3
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    Unknown
    The use of multivariate morphometrics to determine the nutritional condition of marine fish larvae (1991)
    ICES
    In:  [Paper] In: ICES Annual Science Conference 1991, 26.09.1991, La Rochelle, France .
    Details
    Publication Date: 2017-01-06
    Description: The usefulness of multivariate morphometrics to distinguish between fed and starved fish larvae was tested on laboratory reared herring larvae (Clupea harengus ). Linear Discriminant Analysis was used to obtain a linear function which separates the two groups of larvae maximally. The calculations were based on twelve morphometric characters, taken individually by means of an image analysing system. A statistically significant separation of fed and starved larvae was obtained. The most important characters and the number of characters necessary for separation are outlined.
    Type: Conference or Workshop Item , NonPeerReviewed
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  • 4
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    Unknown
    A comparison of the nutritional condition of herring larvae as determined by two biochemical methods - tryptic enzyme activity and RNA/DNA ratio measurements (1992)
    Oxford Univ. Press
    In:  ICES Journal of Marine Science, 49 . pp. 245-249.
    Details
    Publication Date: 2017-06-15
    Description: Two biochemical methods for measuring larval fish condition – tryptic enzyme activity and RNA/DNA ratio measurement - were applied to laboratory-reared and wild-caught herring larvae. The comparison of both methods when applied to laboratory-reared herring larvae showed that tryptic enzyme activity and RNA/DNA ratio are linear and positively correlated under constant nutritional conditions. Wild-caught larvae were transferred to the laboratory and used to compare both indicators in relation to shortterm changes in food availability and long-term starvation periods (13 days). In the starvation experiments with the wild-caught larvae the lowest trypsin values were obtained after 3–4 days and a significant decrease in RNA/DNA ratios was obtained after 5–6 days. Prolongation of the starvation time did not result in a further significant change in either parameter. The results of the study demonstrate the usefulness of both methods in monitoring nutritional condition offish larvae in field samples.
    Type: Article , PeerReviewed
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  • 5
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    Unknown
    Improvements in the fluorimetric determination of the RNA and DNA content of individual marine fish larvae (1993)
    Inter Research
    In:  Marine Ecology Progress Series, 100 . pp. 177-183.
    Details
    Publication Date: 2018-03-07
    Description: The RNA/DNA ratio is a useful indicator of the nutritional condition of fish larvae. The presented analytical procedure is an improvement of Clemmesen's (Meeresforschung 32: 134-143, 1988) methodology which involves purification of fish larvae tissue homogenates and subsequent fluorescence-photometric measurements using specific nucleic acid dyes. The modifications concern the homogenization and nucleic acid extraction procedures. A 'shaking mill' was compared to a potter Elvehjem microhomogenizer and a reduction in the washing and purification steps was achieved. Treatment of samples with ribonuclease A and subsequent fluorescence measurement using ethidium bromide was given preference compared to the DNA-bisbenzimidazole determinations due to problems arising from high self-fluorescence of the samples and the influence of 'quenching' substances disturbing the DNA-bisbenzmidazole determinations. Different RNase concentrations and their influences on RNA and DNA were checked. Recovery rates of standard RNA and DNA 'spikes' were determined. Fish larvae samples were analysed with the previous and the improved modified procedure and a correction factor to compare results measured with the 2 procedures was calculated. With the presented method the physiological condition of individual larvae and the amount of variability can be determined.
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