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  • Articles  (63)
  • 1990-1994  (63)
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  • Articles  (63)
Year
Journal
  • 1
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A simple agar plating method for the description of microbial communities is described. This method is based on the quantification of the numbers of bacterial colonies in 6–7 age-based classes as they appear on agar media over a period of 6–10 days. The method can be used to quantify microbial communities in different habitats (roots and soil) and can be related to the ecophysiology of the microbial communities present. Significant differences in distribution patterns were found in time and depth on the roots. In general, as roots matured, the microbial communities changed from one dominated by r-strategists to one that was more distributed towards K-strategists. The soil had the greatest percentage of organisms that could be characterized as K-strategists. The method was also used to compare microbial communities on wheat roots and in soil in both the field and in microcosms in the glasshouse. In general, the method enabled differentiation between r- and K-strategists in environmental samples, something that could not be done using an ecophysiological index (a modification of the Shannon diversity index) or total bacterial numbers alone.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Microbial ecology 26 (1993), S. 277-285 
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of lettuce (Latuca sativa L.) germination and growth in nonsterilized potting compost of 0.1% and 1.0% w/w incorporation of fermenter biomass inocula of six strains of Trichoderma was investigated. Except for strains WT and T35 at 0.1 % w/w, all inocula inhibited germination. Biomass of strains WT, T35, 20, and 47 at 1.0% promoted shoot fresh weight, whereas strains TH1 and 8MF2 were inhibitory. In contrast, when biomass of strains WT, TH1, and 8MF2 was autoclaved and incorporated at 1%, shoot fresh weight was promoted, but the biomass of T35 was inhibitory. None of the strains incorporated at 0.1 % w/w increased shoot fresh weight, and autoclaved biomass of TH1, T35, and 20 incorporated at 0.1% w/w resulted in lower shoot fresh weights in comparison with uninoculated controls. The shoot dry weight of lettuce seedlings could be enhanced by germinating seeds in uninoculated compost and after five days' growth transferring them into WT-inoculated compost. Inoculum of strain TH1 when applied using this method was very inhibitory. With WT the degree of increase in shoot fresh weight and germination rate declined as the fermentation time to produce inocula was increased.
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  • 3
    ISSN: 1432-0789
    Keywords: Lettuce ; Damping-off ; Biocontrol ; Trichoderma ; Enterobacter ; Gliocladium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Damping-off of lettuce (Lactuca sativa) caused by Pythium ultimum was studied in pots containing a non-sterile potting mix in the glasshouse. Fifty P. ultimum sporangia g-1 compost reduced the plant stand to 15% and shoot dry weight to 18%, but this reduction was totally prevented by applying Trichoderma harzianum at 2×105 viable propagules g-1 potting mix. Gliocladium virens and Enterobacter cloacae also alleviated the damping-off. E. cloacae did not adversely affect the action of the fungal antagonists. The co-existence of the bacterial and fungal antagonists was revealed on the root surface and inner surface of the testa by scanning electron microscopy, indicating the compatibility of the biocontrol agents.
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  • 4
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Isolates of Trichoderma were tested for their ability to control Rhizoctonia solani in lettuce seedlings and mature plants in a glasshouse. The best isolate (T. viride IMI 298375) was tested for its ability to control bottom rot disease in mature lettuce plants grown in polythene tunnels. Control was achieved in several trials but there was little evidence for much improvement after repeated applications and control with Trichoderma was poorer than that obtained with a standard treatment with tolclofos-methyl. Evidence was obtained to suggest however that enhanced growth of lettuce plants following treatment with Trichoderma can produce marketable yields of lettuce similar to those obtained with tolclofos-methyl.
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 97 (1993), S. 11283-11285 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 13 (1990), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Maintenance of realistically low solution P concentrations under controlled conditions is a major difficulty in studies of P nutrition. In this report, we describe a relatively simple and economical sand culture system capable of sustaining plant growth to maturity under controlled yet realistic P regimes. The system uses Al2O3 as a solid-phase P buffer, and modern process control technology to control irrigation and addition of other mineral nutrients. Aspects of the design, use and potential applications of automated solid-phase systems are discussed. The system was used to grow Phaseolus vulgaris to matarity at 0.4 mmol m3, 1.0 mmol m3 and 27 mmol m3 P with and without mycorrhizal inoculation. At flowering, low solution P concentrations were associated with reduced leaf concentrations of P in nonmycorrhizal plants, and reduced leaf concentrations of Ca in both mycorrhizal and nonmycorrhizal plants. Mycorrhizal inoculation increased leaf P, K, Mg and Mn concentrations, but reduced leaf N concentration. Low P regimes reduced final seed yield by diminishing both the number of pods per plant and the number of seeds per pod. Mycorrhizal inoculation significantly enhanced seed yield under low P regimes by increasing seed weight, the number of pods per plant, and the number of seeds per pod.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 8 (1991), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 7 (1991), S. 494-501 
    ISSN: 1573-0972
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Effects of temperature, pH and water potential on blomass production or hyphal extension of Gliocladium virens (G20) and three Trichoderma isolates were determined in vitro. Optimum blomass production occurred between 20 and 30°C and at pH ranges between 4.6 and 6.8. Two isolates of T. viride grew at 5°C and G. virens grew at 35°C but no isolates grew at 40°C. Hyphal extension rates and conidial germination of all fungi declined with decreasing water potential over the range -0.7 to -14.0 MPa. In general, growth rates for each isolate were lower on potato/dextrose agar with water potential adjusted with polyethylene glycol than when adjusted with NaCl or glycerol. No mycelial growth or spore germination occurred on agar at-14.0 MPa.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 10 (1994), S. 20-26 
    ISSN: 1573-0972
    Keywords: Biocontrol ; Botrytis cinerea ; compost ; compost extract
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Manure-straw mixtures were composted and water extracts, made by incubating compost in water for 3 to 18 days, were assessed for antagonistic activity against Botrytis cinerea, using a range of tests. Extracts of all ages inhibited conidial germination on glass slides and reduced mycelial growth on agar. Mixing extracts of all ages with droplets of suspensions of B. cinerea conidia on detached Phaseolus bean leaves suppressed lesion development, but only 3- to 8-day-old extracts had an effect when sprayed onto leaves 2 days before inoculation. Extracts contained a large and varied microbial population of actinomycetes (0.3 to 2.4×105 c.f.u.ml−1), bacteria (1.5 to 5.6×1010 c.f.u.ml−1), filamentous fungi (25.0 to 45.5 c.f.u. ml−1) and yeasts (26.1 to 62.6 c.f.u.ml−1). Eight- and 18-day-old extracts lost activity completely on filter sterilization or autoclaving. Weekly sprays of 8-day-old extracts onto lettuce in the glasshouse had no effect on the incidence of grey mould, but significantly reduced its severity and increased marketable yield. The use of compost extracts in biocontrol of plant diseases and their possible mode of action is discussed.
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  • 10
    ISSN: 1432-0827
    Keywords: Postmenopausal women ; Lumbar vertebrae ; Trabecular organization ; Macroradiography ; Fractal signature analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract High definition macroradiography was used to provide an image of the detailed structural organization of the cancellous bone in human lumbar vertebrae. The fractal signature analysis (FSA) method was used to quantify the horizontal and vertical trabecular organization recorded within the image. Comparison of the FSA of the posteroanterior and lateral macroradiographs in postmortem lumbar vertebrae showed that neither the superimposition of the neural arch nor the radiographic angle affected the trabecular measurement within the vertebral body. FSA analysis of the trabecular structure measured from the macroradiographs of lumbar vertebrae in two groups of postmenopausal women, with high and low bone mineral density (BMD), showed that the large vertical trabecular structures correlated with the women's body weight (P〈0.01–0.03) and body mass index (P〈0.005–0.05), the fine horizontal structures correlated with the women's age (P〈0.005–0.05), and fine vertical trabecular structures were significantly greater (P〈0.005–0.05) in the low compared with the high BMD group.
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