ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
The polysaccharidic effect of a purified 1,3-β-glucanase, a purified β-glucosidase, and of partially purified endo-1,3-β-glucanase from autolysed Penicillium oxalicum cultures on cell wall isolate fractions from the same fungus were studied.Fractionation of 5-day-old cell wall gave rise to a series of fractions that were identified using infrared spectrophotometry. The fractions used were: F1, an α-glucan; F3, a β-glucan; F4, a chitin-glucan; and F4b, a β-glucan. The fractions were incubated with each of the enzymes and with a mixture of equal parts of the three enzymes and the products of the enzymatic hydrolysis were analyzed after 96 h incubation.The enzymes were found to degrade fraction F4b (β-glucan); the greatest degree of hydrolysis was reached when the three enzymes were used together, suggesting the need for synergic action by these enzymes in the cell wall degradation process.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1990.tb13984.x
Permalink