Publication Date:
1991-12-06
Description:
A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement. The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nielsen, P E -- Egholm, M -- Berg, R H -- Buchardt, O -- New York, N.Y. -- Science. 1991 Dec 6;254(5037):1497-500.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry B, Panum Institute, Copenhagen, Denmark.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1962210" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Molecular Sequence Data
;
Molecular Structure
;
Nylons/*chemistry
;
Oligonucleotides/*chemistry
;
Photochemistry
;
Thermodynamics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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