ALBERT

Notes: Abstract Adding fresh roots to intact soil cores resulted in marked increases in microbial and microfaunal activity at the resource islands. Microbial activity increased in two phases following root addition. Respiratory activity and concentration of respiratory enzyme (dehydrogenase) in soil adhering to the roots was very high during the first three weeks resulting in anaerobic conditions in the soil. After a period of low respiratory activity and enzyme content, these quantities increased from 6 to 20 weeks, but not enough to maintain anaerobic conditions. Numbers of protozoa peaked earlier than the nematodes. Based on yield coefficients of microbes and bacterivores, the increase in bacterivores was in accordance with root-induced respiration activity. In soil adhering to roots, numbers of bacterial grazers (protozoa and nematodes) were up to 80 and 30 times higher, respectively, than in the surrounding soil. This effect is up to 20 times higher than observed around live root systems, which may suggest that the rhizosphere effect on microbivores could for the major part result from the decomposition of dead segments of the root system.

Notes: . Species of Tetrahymena, including T. vorax, T. thermophila, T. pyriformis, and T. pigmentosa, were tested for cloning efficiency in proteose peptone and in synthetic nutrient media to which were added hemin, protoporphyrin IX, chlorophyllin, or asolectin, an impure mixture of phospholipids. All species could be cloned with high efficiency in the crude media. In unsupplemented synthetic medium the cloning efficiencies were 0–10%, around 50%, around 50%, and 90–100% for T. thermophila, T. vorax, T. pyriformis, and T. pigmentosa, respectively. The first three were all stimulated to 90–100% by addition of the porphyrin or phospholipid compounds mentioned above. Uroporphyrin III and coproporphyrin I and III had no effect. We suggest that cells unable to form clones suffer from a lack of cellular energy. This situation may be alleviated by our additions: certain porphyrin rings may be built into cytochromes and phospholipids may be used as fuel. Thus, the synthetic media used so far for these ciliates have not been optimal.

Notes: In dose-response experiments with the herbicide combinations MCPA+dichlorprop and ioxynil+mecoprop in barley, winter wheat, and winter rye varieties, oilseed rape (Brassica napus L.) was used as a substitute for weeds. The results showed a significant interaction between the competitive ability of varieties and herbicide performance. A negative exponential model with variety as categorical variable was used to analyse the data and to assess the competitive effect of the varieties on herbicide efficacy.A target level of 5 g weed dry matter m−2 in all varieties at the end of flowering was used to derive a varietal calibration factor of the herbicide dose. The results showed significant differences in calibration factors between species of winter wheat, barley, and rye, and between varieties of spring barley and winter wheat. In the winter wheat variety Sleipner it was necessary to use a 154% higher herbicide dose than in the winter barley variety Trixi, whereas in the winter rye variety Petkus II, herbicide dose could be reduced by 31% compared with Trixi. The differences between spring barley varieties were smallerbut significant. Competition entre culture et mauvaises herbes, et efficacité herbicide chez différentes espèces et variétés de céréales Dans des expériences dose-effet avec les combinaisons herbicides MCPA+dichlorprop et ioxynil+mécoprop dans différentes variétés d'orge, de blé d'hiver et de seigle d'hiver, le colza (Brassica napus L.) était utilisé comme modèle de mauvaise herbe. Les résultats ont montré une interaction significative entre l'aptitude à la concurrence des variétés et l'efficacité herbicide. Un modèle exponentiel négatif avec la variété comme variable catégorielle, a été utilisé pour analyser les données et pour déterminer l'effet de l'aptitude à la compétition des variétés sur l'efficacité herbicide.Un objectif de 5 g de matière sèche de mauvaise herbe par m2 pour toutes les variétés à la fin de la floraison a été utilisé pour déterminer un facteur de calibration varietal de la dose d'herbicide. Les résultats ont montré des différences significatives entre les facteurs de calibration déterminés pour les espèces blé d'hiver, orge et seigle, ainsi qu'entre les variétés d'orge de printemps et de blé d'hiver. Avec la variété de blé d'hiver Sleipner, U était nécessaire d'utiliser une dose d'herbicide supérieure de 154%à celle nécessaire pour la variété d'orge d'hiver Trixi, alors que dans le seigle d'hiver Petkus II, la dose herbicide pouvait ßtre réduite de 31 % par rapport à Trixi. Les différences entre variétés d'orge de printemps étaient plus faibles mais significatives. Konkurrenz zwischen Kulturpflanzen und Unkraut und Herbizidwirkung bei Getreidearten und -Sorten In Dosis-Wirkungs-Untersuchungen mit den Herbiziden MCPA+Dichlorprop und loxynil+Mecoprop wurde in Gersten-, Winterweizen- und Winterroggensorten Raps (Brassica napus L.) als Unkraut eingesetzt. Die Ergebnisse zeigten eine signifikante Wechselwirkung zwischen der Konkurrenzkraft von Sorten und der Herbizidwirkung. Ein negativ exponentielles Modell mil der Sorte als Kategorie wurde angewandt, um die Da ten zu analysieren und den Einfluß der Sorten auf die Herbizidwirksamkeit zubestimmen. Es wurde eine Zielgröße von 5 gm−2 Unkrauttrockenmasse in allen Sorten zum Endeder Blüte gesetzt, um einen sortenbezogenen Kalibrierungsfakt or für die Herbiziddosis zu erhalten. Es wurden signifikante Unterschiede der Kalibrierungsfaktoren zwischen den Getreidarten Winterweizen, Gerste und Roggen und zwischen den Sommergersten- und Winterweizensorten gefunden. Bei der Winterweizensorte Sleipner war es erforderlich, eine 154 % höhere Herbiziddosis als bei der Wintergerstensorte Trixi anzuwenden, während die Herbiziddosis bei der Winterroggensorte Petkus II im Vergleich zu Trixi um 31 % reduziert werden konnte. Die Unterschiede waren bei Sommergerstensorten geringer, jedoch signifikant.

Notes: Pyridoxylated adult human hemoglobin (HbAo) was prepared using a one molar equivalent of pyridoxal 5-phosphate (PLP) per heme and reduced with either NaCNBH3 or NaBH4. A separate sample was pyridoxylated and passed through a mixed-bed ion exchange column without reduction. All three preparations had a P50 of 29 ± 2 torr and a cooperativity of n = 2.4 ± 0.1. These preparations, in both the oxy and deoxy forms, were then treated with 7 equivalents of glutaraldehyde per tetramer at pH 6.8 at 4°C and at room temperature. The polymerization invariably reduced the P50 to 18 ± 2 torr with Hill coefficients of less than 2. These solutions, with or without further reduction using NaCNBH3, all retained the PLP in differing amounts (2-3 moles/tetramer). Methemoglobin concentrations were increased during the polymerization reaction. The normal pyridoxylation procedure, using sodium borohydride reduction, resulted in a number of different molecular species. Polymerization with glutaraldehyde caused a further proliferation of molecular species that could not be separated by anion exchange chromatography or by isoelectric focusing. The extent of polymerization, estimated by gel exclusion chromatography and SDS polyacrylamide gel electrophoresis, was from 40 to 50%. Analysis of the reverse phase chromatograms, which separate the heme and the α- and β-chains, showed extensive polymerization and distribution of the radioactively labeled PLP on the protein for all preparations. All of the polymerized and pyridoxylated samples were unstable, and showed different chromatographic patterns after storage at 4°C for 1 month. Attempts to stabilize these preparations by further reduction with NaCNBH3 gave products with a lower P50 and lower cooperativity. When the reactions were conducted with a purified HbAo, heterogeneity was somewhat decreased compared to the normally used stroma-free hemoglobin, but a large number of molecular species were still formed.