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  • Springer  (107)
  • American Meteorological Society (AMS)
  • 1990-1994  (71)
  • 1970-1974  (22)
  • 1935-1939  (14)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Purines and cortisone in lipid mobilization (1970)
    Springer
    Cellular and molecular life sciences 26 (1970), S. 128-129 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Purin-Basen, Kaffein und Cortison führen zu einer Vermehrung der Fettsäuren im Rattenserum. Adenin vermindert im Gegensatz zu den anderen Purinen die Lipolyse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01895532
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  • 2
    Electronic Resource
    Electronic Resource
    Ion association reactions with biological membranes, studied with the fluorescent dye 1-anilino-8-naphthalenesulfonate (1970)
    Springer
    The journal of membrane biology 3 (1970), S. 241-266 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary (1) When salts are added to buffered suspensions of membrane fragments containing the fluorochrome 1-anilino-8-naphthalenesulfonate (ANS), there is an increased fluorescence. This is caused by increased binding of the fluorochrome; the intrinsic fluorescence characteristics of the bound dye remain unaltered. These properties make ANS a sensitive and versatile indicator of ion association equilibria with membranes. (2) Alkali metal and alkylammonium cations bind to membranes in a unique manner. Cs+ binds most strongly to rat brain microsomal material, with the other alkali metals in the order Cs+〉Rb+〉K+〉Na+〉Li+. The reaction is endothermic and entropy driven. Monovalent cations are displaced by other monovalent cations. Divalent cations and some drugs (e. g., cocaine) displace monovalent cations more strongly. (3) Divalent cations bind to membranes (and to lecithin micelles) at four distinct sites, having apparent association constants between 50 and 0.2mm −1. The characteristics of the titration suggest that only one species of binding site is present at any one time, and open the possibility that structural transitions of the unassociated coordination sites may be induced by divalent cation binding. Divalent cation binding at the weakest site (like monovalent cation binding) is endothermic and entropy driven. At the next stronger site, the reaction is exothermic. Monovalent cations affect divalent cation binding by reducing the activity coefficient: they do not appear to displace divalent cations from their binding sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868018
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  • 3
    Electronic Resource
    Electronic Resource
    Ewing's tumor X mouse hybrids expressing the MIC2 antigen: analyses using fluorescence CDD-banding and non-isotopic ISH (1992)
    Springer
    Human genetics 〈Berlin〉 88 (1992), S. 273-278 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We present a highly sensitive method that has been applied to map the chromosomal origin of the prominent cell surface antigen of Ewing's tumor cells recognized by monoclonal antibody HBA-71. The technique allows an unambiguous identification of human chromosomal material in interspecific cell hybrids. This is achieved by fluorescent in situ hybridization of biotinylated total human DNA, followed by high resolution fluorescence banding with the chromomycin/distamycin/ DAPI triple stain. An advantage of this method is that all signals can be visualized in one single operation by simply switching the appropriate filter blocks. The protocol has proved extremely useful in gene mapping by means of interspecific cell hybrids, a technique that depends on the accurate and unambiguous recognition of the relevant (e.g., human) genetic material in the clonal genome. Our studies confirm that this antigen is the product of the MIC2 gene, which is so far the only well-studied pseudoautosomal gene in man, and which is located on the short arms of chromosomes X and Y. Furthermore, no influence of the Ewing's tumor-specific translocation t(11;22) on MIC2 expression could be discerned.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197258
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  • 4
    Electronic Resource
    Electronic Resource
    A method for the isolation of nuclei from the dermatophytic fungus,Microsporum gypseum (1974)
    Springer
    Archives of microbiology 96 (1974), S. 281-289 
    Details
    ISSN: 1432-072X
    Keywords: Fungal Nuclei ; Isolation ; Purification ; Characterization ; Dermatophyte ; Microsporum gypseum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is reported for the isolation of nuclei from the dermatophytic fungus,Microsporum gypseum. The yield ranged from greater than 90% in crude preparations to less than 20% in highly purified preparations as judged by the recovery of DNA. Nuclei isolated in this manner appeared intact in electron microscopic observations and possessed chemical compositions, i.e. low protein/DNA and RNA/DNA ratios, which indicated a low degree of cytoplasmic contamination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00590184
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  • 5
    Electronic Resource
    Electronic Resource
    Physiological aspects of glyphosate degradation in Alcaligenes spec. strain GL (1990)
    Springer
    Archives of microbiology 153 (1990), S. 146-150 
    Details
    ISSN: 1432-072X
    Keywords: Alcaligenes spec. GL ; Phosphonate metabolism ; Glyphosate ; Sarcosine ; Phosphate starvation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Alcaligenes spec. strain GL (IMET 11314) is able to grow on glyphosate (N-[phosphonomethyl]glycine) and other phosphonates as sole source of phosphorus. Degradation of glyphosate to inorganic phosphate and sarcosine by this strain is subject to several regulatory principles. While uptake and dephosphonation of glyphosate are regulated by Pi starvation, the intensity of glyphosate degradation is also controlled by the cellular ability to utilize the C-skeleton derived from glyphosate. Depending on the external concentration of glyphosate, the liberated sarcosine is differentially metabolised. Utilization of the sarcosine moiety and complete incorporation of 3-[14C]-label of glyphosate into cellular material occur only in cultures adapted to higher concentrations (5 mM) of the herbicide. At low concentrations of glyphosate (1 mM) only the Pi required by the growing cultures is utilized but not the sarcosine. Initially high rates of glyphosate uptake obtained after Pi-starvation decrease in the presence of low glyphosate concentrations. It is suggested that uptake and metabolism of glyphosate are connected with the expression of the sarcosine metabolizing capacity of the Alcaligenes cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00247812
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  • 6
    Electronic Resource
    Electronic Resource
    Thermal stability of Mo/Si multilayer soft-X-ray mirrors fabricated by electron-beam evaporation (1994)
    Springer
    Applied physics 58 (1994), S. 371-376 
    Details
    ISSN: 1432-0630
    Keywords: 68.55 ; 68.65 ; 78.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Mo/Si multilayers are fabricated by electron-beam evaporation in UHV at different temperatures (30° C, 150° C, 200° C) during deposition. After completion their thermal stability is tested by baking them at temperatures (T bak) between 200° C and 800° C in steps of 50° C or 100° C. After each baking step the multilayers are characterized by small angle CuKα-X-ray diffraction. Additionally, the normal incidence soft-X-ray reflectivity for wavelengths between 11 nm and 19 nm is determined after baking at 500° C. Furthermore, the layer structure of the multilayers is investigated by means of Rutherford Backscattering Spectroscopy (RBS) and sputter/Auger Electron Spectroscopy (AES) technique. While the reflectivity turns out to be highest for a deposition temperature of 150° C, the thermal stability of the multilayer increases with deposition temperature. The multilayer deposited at 200° C stands even a 20 min 500° C baking without considerable changes in the reflectivity behaviour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00323612
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  • 7
    Electronic Resource
    Electronic Resource
    Pharmacokinetics of S(+)- and R(−)-ibuprofen in volunteers and first clinical experience of S(+)-ibuprofen in rheumatoid arthritis (1990)
    Springer
    European journal of clinical pharmacology 38 (1990), S. 493-497 
    Details
    ISSN: 1432-1041
    Keywords: ibuprofen ; rheumatoid arthritis ; enantiomer ; stereoselectivity ; pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary S(+)-, R(−)- or racemic ibuprofen was administered orally to volunteers in doses of 150 mg, 300 mg and 500 mg pure S(+)-, 300 mg pure R(−)- and 600 mg racemic ibuprofen. The pharmacokinetic parameters in humans showed that S(+)-ibuprofen was not inverted to R(−)-ibuprofen, whereas R(−)-ibuprofen was inverted to S(+)-ibuprofen to a variable degree. S(+)-ibuprofen and R(−)-ibuprofen given alone more rapidly reached significantly higher maximal plasma concentrations than after the same doses of the racemic compound. The elimination half-lives and clearance values for all three forms of ibuprofen were comparable. The mean residence time of S(+)-ibuprofen after R(−)- and racemic ibuprofen was significantly longer than after administration of the pure S(+)-enantiomer. Judged by the AUC, the bioavailability of S(+)-ibuprofen was independent of the dose within the range tested. Administration of S(+)-ibuprofen to 6 rheumatic patients showed that the pharmacokinetic behaviour of S(+)-ibuprofen in patients was similar to that found in volunteers. S(+)-ibuprofen proved to be an effective analgesic antirheumatic drug in the dose range 1 to 1.5 g/day.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02336690
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  • 8
    Electronic Resource
    Electronic Resource
    Rheological measurements and light-scattering experiments of dilute solutions of high molecular polystyrene. Light-scattering of flowing polymer solutions (1991)
    Springer
    Colloid & polymer science 269 (1991), S. 1250-1256 
    Details
    ISSN: 1435-1536
    Keywords: Light-scattering ; flowing polymer solution ; dilute polymer solution ; orientation and deformation of macromolecules ; polystyrene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract We describe the behavior of dilute polymer solutions by means of light-scattering under shear flow. Solution properties of polystyrene in benzene over a wide range of molecular weight has been studied to determine the coefficientsa andK of the Mark-Houwink relationship and to estimate the rheological conditions with regard to light-scattering experiments of flowing polymer solutions. The investigations were carried out to measure the shear-rate dependence of macromolecules in solution, e.g., to observe an orientation and changing of the mean-square radius of gyration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00652533
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  • 9
    Electronic Resource
    Electronic Resource
    Ladungszahl und Polymerisationsgrad des Metawolframations in wäßriger NaCl-haltiger Lösung nach Untersuchungen des Sedimentationsgleichgewichtes mit der Ultrazentrifuge (1971)
    Springer
    Colloid & polymer science 249 (1971), S. 1148-1154 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Summary The chargez of the meta-tungstate ion in the ternary system Na6[H2W12O40]-NaCl-H2O is determined by ultracentrifugation experiments, evaluating the experimental data: 1. according to the limiting value ofLamm by eytrapolating the apparent molecular weight to zero-concentration, 2. according to the procedure ofJohnson, Kraus andScatchard. The discrepancy of the results ¦z¦=6 versus ¦z¦=2,85 is resolved by giving the proof that the latter value has no physical significance. For the molecular weight the first method gives the exact value, the second furnishes a very satisfying approximation.
    Notes: Zusammenfassung Die Ladungszahlz des Metawolframations in dem ternären System Na6[H2W12O40]-NaCl-H2O wird nach zwei verschiedenen Verfahren durch Untersuchung des Sedimentations—Diffusionsgieichgewichtes mit der Ultrazentrifuge bestimmt: 1. Mit Hilfe der Lammschen Grenzbeziehung durch Extrapolation der scheinbaren Molmasse auf die Konzentration Null und 2. nach der Methode vonJohnson, Kraus undScatchard. Die Ergebnisse sind ¦z¦=6 bzw. ¦z¦=2,85. Es wird gezeigt, daß dem nach dem Verfahren vonJohnson, Kraus undScatchard erhaltenen Wert ¦z¦=2,85 keine physikalische Realitat zukommt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01556986
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  • 10
    Electronic Resource
    Electronic Resource
    Book reviews (1994)
    Springer
    Chromatographia 39 (1994), S. 375-375 
    Details
    ISSN: 1612-1112
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02274529
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