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  • 1
    ISSN: 1432-1432
    Keywords: CpG dinucleotides ; Evolution ; Repetitive sequences ; Reverse transcriptase ; S1Bn retroposons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The identification of a family of SINE retroposons dispersed in the genome of oilseed rape Brassica napus has provided the basis for an evolutionary analysis of retroposition in plants. The repetitive elements (called S1Bn) are 170 by long and occupy roughly 500 loci by haploid genome. They present characteristic features of SINE retroposons such as a 3′ terminal A-rich region, two conserved polymerase III motifs (box A and B), flanking direct repeats of variable sizes, and a primary and secondary sequence homology to several tRNA species. A consensus sequence was made from the alignment of 34 members of the family. The retroposon population was divided into five subfamilies based on several correlated sets of mutations from the consensus. These precise separations in subfamilies based on “diagnostic” mutations and the random distribution of mutations observed inside each subfamily are consistent with the master sequence model proposed for the dispersion of mammalian retroposons. An independent analysis of each subfamily provides strong evidence for the coexpression of at least three subfamily master sequences (SMS). In contrast to mammalian retroposition, diagnostic positions are not shared between SMS. We therefore propose that SMS were all derived from a general master sequence (GMS) and independently activated for retroposition after a variable period of random drift. Possible models for plant retroposition are discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Brassica napus ; Raphanus sativus ; Ogura cytoplasmic male-sterility restorer gene ; Bulked segregant analysis ; RAPD markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bulked segregant analysis was employed to identify random amplified polymorphic DNA (RAPD) markers linked to the restorer gene (Rfo) used in theOgura radish cytoplasmic male sterility of rapeseed. A total of 138 arbitrary 10-mer oligonucleotide primers were screened on the DNA of three pairs of bulks, each bulk corresponding to homozygous restored and male sterile plants of three segregating populations. Six primers produced repeatable polymorphisms between paired bulks. DNA from individual plants of each bulk was then used as a template for amplification with these six primers. DNA polymorphisms generated by four of these primers were found to be completely linked to the restorer gene with the polymorphic DNA fragments being associated either with the fertility restorer allele or with the sterility maintainer allele. Pairwise cross-hybridization demonstrated that the four polymorphic DNA fragments did not share any homology. Southern hybridization of labelled RAPD fragments on digested genomic DNA from the same three pairs of bulks revealed fragments specific to either the male sterile bulks or to the restored bulks and a few fragments common to all bulks, indicating that the amplified sequences are low copy. The four RAPD fragments that were completely linked to the restorer locus have been cloned and sequenced to develop sequence characterized amplified regions (SCARs). This will facilitate the construction of restorer lines used in breeding programs and is the first step towards map-based cloning of the fertility restorer allele.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 89 (1994), S. 847-852 
    ISSN: 1432-2242
    Keywords: RAPD ; DNA fingerprinting ; Phylogeny Apple rootstocks ; Identification key
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RAPD (random amplified polymorphic DNA) markers were used to fingerprint eight commercially available apple rootstocks (Nertchinsk, Northern Spy, Osman, Heyer 12, M.1, M.9, M.26 and MM.106), 10 winter hardy offsprings derived from the cross of Nertchinsk x M.9, six winter hardy offsprings derived from the cross of Nertchinsk x M.26 and one winter hardy offspring derived from each of the two crosses between Osman x Heyer 12 and Northern Spy x M.1. Phylogeny analysis using parsimony allowed us to draw the genetic relationship between these lines using only RAPD markers data. The resulting cladogram was compared to the true genetic relationship between these lines in order to assess the efficiency of RAPD markers in determining accurately the phylogenetic relationship. We also developed a DNA fingerprinting system based on 13 informative RAPD loci amplified by five RAPD primers that allowed the rapid identification of apple rootstocks.
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  • 4
    Publication Date: 2014-04-08
    Print ISSN: 0261-4189
    Electronic ISSN: 1460-2075
    Topics: Biology , Medicine
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  • 5
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    In:  Zoologische Verhandelingen vol. 276 no. 1, pp. 1-42
    Publication Date: 2024-01-12
    Description: The habitus and genitalia are illustrated for the twelve species of Pterophoridae known from the Gal\xc3\xa1pagos Islands (Ecuador). The status of one species is discussed, as it may be the unknown female of Postplatyptilia minima spec. nov. Five species are described as new: Postplatyptilia huigraica, P. minima, Platyptilia nigroapicalis, Oidaematophorus cristobalis and O. devriesi. Redescriptions and/or diagnoses are given for the other seven species. Lectotypes are designated for Pterophorus nephogenes Meyrick and Platyptilia brevipennis Zeller. A key based on adult external features is provided. Four species were found to be possibly endemic to the archipelago. New foodplant records are given for two species.
    Keywords: Lepidoptera ; Pterophoridae ; plume-moths ; Gal\xc3\xa1pagos Islands ; new species ; key.
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 6
    Publication Date: 2024-01-12
    Description: Two new species of Pterophoridae (Platyptilia vilema spec. nov. and Stenoptilodes gielisi spec, nov.) are described from the Gal\xc3\xa1pagos Islands. Exelastis pumilio (Zeller) is reported from the archipelago for the first time. Seven hostplant records (six of them new) are given for five of the 15 species now found in the Gal\xc3\xa1pagos. Twenty-three new island records are given for species already mentioned from the archipelago.\nSe describen dos especies nuevas de Pterophoridae (PlatypHlia vilema spec. nov. y Stenoptilodes gielisi spec. nov.) para las islas Gal\xc3\xa1pagos. Exelastis pumilio (Zeller) se cita por primera vez para el archipi\xc3\xa9lago. Se dan siete registros de plantas hospederas, de las cuales seis son nuevos, para cinco de las 15 especies conocidas. Se presentan 23 nuevos registros de distribution dentro del archipi\xc3\xa9lago, por especies previamente mencionadas.
    Keywords: Lepidoptera ; Pterophoridae ; Gal\xc3\xa1pagos Islands ; new species ; new distribution records ; new hostplant records
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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