ISSN:
1615-6102
Keywords:
NADPH oxidase
;
Superoxide
;
Kidney mesangial cell
;
Fibroblast
;
B lymphocyte
;
Endothelial cell
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary NADPH oxidase of phagocytic leucocytes contains a membrane cytochromeb with two subunits, gp91 phox and p22 phox , together with three cytosolic proteins, p47 phox , p67 phox and p2 rac . The presence of some of these components has been sought in non-phagocytes, using Western blot analysis for protein expression and PCR to amplify and detect mRNA. All components were detected in EBV-transformed B lymphocytes and peripheral blood B lymphocytes. Fibroblasts and human kidney mesangial cells contained mRNA for p67 phox , p47 phox , and p22 phox but not gp91 phox . Levels of expression varied with growth conditions, but it appears possible than an isozyme of cytochromeb which lacks gp9 phox is present in these cells. Proteins of p47 phox and p67 phox were expressed, in low concentrations, in these two cell types. Expression of mRNA for p47 phox and p67 phox was found to be widespread in many cell types.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01276904
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