ISSN:
1076-5174
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
The application of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for quantitative analysis was investigated with the use of internal standards. Three peptides/proteins in the mass range 1000-12000 were tested and the effect of various internal standards was evaluated. Horse cytochrome c was used as an internal standard for bovine cytochrome c, melittin for renin and an undecapeptide B analogue was employed as an internal standard for the decapeptide A. A linear response was found between the measured peak height ratio and the applied amount of analyte when an appropriate internal stanard was used. The quantitative abilities of MALDI-MS were finally applied to the determination of the drug amperozide in plasma. The biological samples were prepared for analysis using liquid-liquid extraction prior to MALDI-MS. A linear calibration graph was obtained using the 13C4 stable isotopically labelled amperozide as an internal standard.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jms.1190300219
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