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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 43 (1995), S. 2758-2761 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A transformation system has been developed for the pathogen fungus Botrytis cinerea, based on the utilization of the wide host plasmid pUT737 that contains the Sh ble gene, conferring resistance to phleomycin. Transformed protoplasts were regenerated at 10–25 μg ml−1 of phleomycin, at a frequency of 25–40 transformants per μg of DNA, and they were resistant up to 50 μg ml−1. Southern hybridization using undigested and digested total DNA showed the presence of circular autonomously replicating plasmid pUT737 in the transformants. Reisolated plasmid from transformed fungus transformed E. coli and rescued plasmid was identified as pUT737. Transformants were grown for four generations under non-selective conditions and replicative plasmids were still detected. Plasmids present in all transformants at this stage had been modified from native pUT737 and showed the same size and configuration indicating that selection through stabilizing plasmid forms has happened.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The usefulness of artificial neural networks (ANN) for milk shelf-life prediction by multivariate interpretation of gas chromatographic profiles and flavor-related shelf-life was evaluated and compared to principal components regression (PCR). The training set consisted of dynamic headspace gas chromatographic data collected during storage of pasteurized milk (input information for the neural network used to make a decision) and its corresponding shelflife (prediction or response). ANN had better predictability than PCR. A standard error of the estimate of 2 days in shelf-life resulting from regression analysis of experimental vs predicted values indicated a high predictability of ANN.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 24 (1997), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A crucial step in converting an actively growing Bacillus subtilis cell into a dormant spore is the formation of a cell within a cell. This unusual structure is created by a phagocytosis-like process in which the larger mother cell progressively engulfs the adjacent smaller forespore. Only mutations blocking engulfment at an early stage and affecting genes expressed in the mother cell have been identified. Here we describe a new locus, spoIIQ, which is transcribed in the forespore and which encodes a membrane-bound protein required at a late stage of engulfment. Immunofluorescence microscopy analysis have shown that SpoIIQ is initially targeted to the septum at the boundary between the two cells and then spreads around the entire membrane of the forespore. Septum targeting requires only the first 52 residues of SpoIIQ as well as unidentified forespore-specific components. Electron-microscopy studies of cells engineered to activate the mother-cell program of gene expression independently of the forespore indicate that other as yet uncharacterized genes are involved in engulfment and that this morphological process is driven from both sides of the forespore envelope.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1612-1112
    Keywords: Gel permeation chromatography ; Tannins in eucalyptus bark ; Polyphenols ; Molecular weight distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The molecular weight distribution of tannins from the wood, bark and leaves ofEucalyptus camaldulensis, E. globulus andE. rudis from two different Spanish provenances has been studied by high performance gel permeation chromatography, using the compounds' acetylated derivatives. The MW distribution profiles showed important variability depending on the type of vegetal tissue, the species and, in some cases, on the geographical provenance of the samples. Bark was the vegetal tissue that yielded tannins with the highest molecular weight, followed by wood and leaves. Tannins from wood and bark ofE. camaldulensis were of higher molecular size than those fromE. globulus andE. rudis; those in the leaves ofE. globulus andE. camaldulensis were similar in molecular size and larger than those in the leaves ofE. rudis.
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  • 6
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SirThe University of El Salvador and its Faculty of Medicine and the National University of Honduras and its Faculty of Medical Sciences wish to express their deep regret and strong protest at the exclusion of Salvador Moncada from the 1998 Nobel Prize in Physiology or ...
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1572-8773
    Keywords: catabolism ; deferrioxamine B ; nutrient recycling ; siderophore
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Most siderophores are catabolism resistant because of their non-peptidic or cyclic peptide chemical structures. Siderophore degradation is thus a rare event, and one which has received little attention. Based on literature precedence and the generation of monohydroxamic acid(s) by cell-free extracts of a deferrioxamine B degrading bacterium, a catabolic scheme of the siderophore is proposed. Data are also presented concerning parameters such as the pH profile, inducible nature of the siderophore-degrading metabolism and the ability of the deferrioxamine B degrading bacterium to metabolize some of the hypothesized products of deferrioxamine B dissimilation.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Marketing letters 6 (1995), S. 221-233 
    ISSN: 1573-059X
    Keywords: Price promotions ; impact of promotions ; decision making under ambiguity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract This paper shows that imprecisely stated discounts in brand promotions offered in the form of a low-probability lottery can lead to higher sales (purchase intentions) and consequently profits than equally costly conventional promotions offering a precise discount on the entire stock. Results from two different experimental studies support our findings. For high-probability lottery-like promotions, imprecise discounts lead to a lower performance for the brand than conventional promotions. We attempt to explain the findings by drawing on the behavioral decision theory literature.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-0867
    Keywords: nitrate leaching ; pollution ; fertilizer ; irrigation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A nitrogenous controlled release fertilizer (Floranid 32) and a treatment of municipal organic waste compost were tested under two irrigation managements (conventional and ET-adjusted irrigation rates) with the aim of assessing risk of nitrate leaching to the aquifer. A check without N fertilizer was introduced. The experiment was carried out at La Poveda Field Station (30 km SE Madrid, Spain) in alluvial soils with water table depth at 4 m and under maize cropping. The experiment was laid out in a randomized complete block design with three replications, allocating 12 plots to each irrigation management. Although N fertilizer rate (150 kg ha−1) was reduced at half as related to a previous experiment, no difference in grain yields was observed. This result relates to a high content of soil-N. Floranid showed promising results in controlling N-leaching in comparison with urea that exhibited an accelerated rate of N release which finally determines low use of N by the plant and marked NO3 − leaching. Treatment of municipal waste compost showed NO3 − concentrations in the soil water solution of similar values as those of urea at 140 cm. ET-adjusted irrigation showed no drainage during the corn growing season and lower NO3 − concentrations in the soil water solution which could indicate a general lower rate of N solubilization.
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  • 10
    ISSN: 1572-8080
    Keywords: Lotos ; system-level design ; co-design ; prototyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract In this paper we present an extension to the co-design approach based on LOTOS presented in Fourth International Workshop on Hardware-Software Co-Design, 1996. In this new version we add a prototyping stage to our design flow, that allows to validate the design at the implementation level. We present the complete approach, stressing the prototyping stage after partitioning. An example of an Ethernet bridge serves us to illustrate our approach and present some results.
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