Publication Date:
1996-10-25
Description:
Tat may stimulate transcriptional elongation by recruitment of a complex containing Tat-SF1 and a kinase to the human immunodeficiency virus-type 1 (HIV-1) promoter through a Tat-TAR interaction. A complementary DNA for the cellular activity, Tat-SF1, has been isolated. This factor is required for Tat trans-activation and is a substrate of an associated cellular kinase. Cotransfection with the complementary DNA for Tat-SF1 specifically modulates Tat activation. Tat-SF1 contains two RNA recognition motifs and a highly acidic carboxyl-terminal half. It is distantly related to EWS and FUS/TLS, members of a family of putative transcription factors with RNA recognition motifs that are associated with sarcomas.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhou, Q -- Sharp, P A -- AI32486/AI/NIAID NIH HHS/ -- CA14051/CA/NCI NIH HHS/ -- GM34277/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1996 Oct 25;274(5287):605-10.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8849451" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
DNA, Complementary/genetics
;
Electrophoresis, Polyacrylamide Gel
;
Gene Expression
;
Gene Products, tat/*genetics
;
HIV Long Terminal Repeat
;
HIV-1/*genetics
;
HeLa Cells
;
Heterogeneous-Nuclear Ribonucleoproteins
;
Humans
;
Immunoblotting
;
Molecular Sequence Data
;
Molecular Weight
;
Neoplasm Proteins/chemistry
;
Phosphorylation
;
Promoter Regions, Genetic
;
Protein Kinases/metabolism
;
RNA, Viral/metabolism
;
RNA-Binding Protein EWS
;
RNA-Binding Protein FUS
;
Ribonucleoproteins/chemistry
;
Sequence Homology, Amino Acid
;
Trans-Activators/chemistry/*genetics/metabolism
;
*Transcriptional Activation
;
Transfection
;
tat Gene Products, Human Immunodeficiency Virus
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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