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  • 1
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Using fluorescence in situ hybridization, conventional epifluorescence microscopy, and laser scanning confocal microscopy followed by three-dimensional reconstruction we describe a well-defined higher order packaging of the human genome in the sperm cell nucleus. This was determined by the spatial localization of centromere and telomere regions of all chromosomes and supported by localization of subtelomere sequences of chromosome 3 and the entire chromosome 2. The nuclear architecture in the human sperm is characterized by the clustering of the 23 centromeres into a compact chromocenter positioned well inside the nucleus. The ends of the chromosomes are exposed to the nuclear periphery where both the subtelomere and the telomere sequences of the chromosome arms are joined into dimers. Thus chromosomes in the human sperm nucleus are looped into a hairpin-like configuration. The biological implications of this nuclear architecture in spermatogenesis and male pronuclear formation following fertilization are discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1090-6509
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Quantum de Haas-van Alphen oscillations and semiclassical angular magnetoresistance oscillations are observed in the quasi-two-dimensional organic metal κ-(BETS)2GaCl4. The behavior of these oscillations attests to the existence of two cylindrical Fermi surface sheets with axes perpendicular to the conducting plane. The cross-sectional areas of these cylinders in the conducting plane are equal to about 20 and 100% of the corresponding cross section of the Brillouin zone. It is shown that the many-particle interaction can be suppressed significantly in this compound.
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  • 3
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract This paper deals with the characterization of mechanical properties of impacted morsellized cancellous allograft (IMCA) produced by dynamic compaction of allograft femoral heads ground by commercially available bone mills, i.e. rotating rasp and reciprocating type bone mills. Various ranges and profiles of particle size in the graft aggregates were obtained using these bone mills, and the effect of number of compaction as well as the distribution of particle sizes on the mechanical properties of IMCA under quasistatic compression and shear loading conditions was discussed. The morsellized cancellous allograft prepared by the reciprocating type bone mill showed a broad distribution of particle sizes, and gave IMCA superior mechanical properties to the graft with a more uniform size distribution, or prepared by the rotating rasp type bone mills. The increase of number of compaction also improved the mechanical properties of IMCA in compression.
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  • 4
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract An attempt was made to assess the local chronic inflammatory response in patients with failed orthopaedic implant that is clinically associated with osteolysis, bone and bone marrow necrosis. The main objective was to analyse the heterogeneity of the macrophage functional subsets in the bone–implant interface membrane and to evaluate their possible role in the development of an erosive inflammatory lesion within the bone. Immunohistology was performed on 21 specimens of the bone–implant interface obtained from 17 patients during revision arthroplasty, and synovial membranes from rheumatoid (RA, n=4), and osteoarthritis (OA, n=4) patients. Three well-characterized monoclonal antibodies (MAb) recognizing antigenic determinants on specific functional subsets of macrophages (Mφ) were used. RFD1 (interdigitating reticulum cells/antigen presenting cells, (APC), RFD7 (mature phagocytic macrophages), and RFD9 epithelioid cells and foreign body giant cells (FBGC). RFD1 was expressed on a variable number of perivascular and synovial lining Mφ in both RA and OA synovia, at a frequency of 25%–40%. In cases with total joint replacements, the interface showed a marked increase in the expression of RFD1 (20%–90%). A considerably greater percentage of RFD1 positive Mφ and FBGC was noted in the interfaces from cases with a high level of detectable metal particulate wear debris (mean 80%, range 60%–90%) than in cases with polyethylene wear debris (mean 30%, range 0%–50%), p 0.0001. RFD7 labelled most tissue Mφ in each group. Immunoreactivity for RFD9 was restricted to FBGC in all cases analysed. The finding of elevated expression of RFD1 on metal-containing Mφ and FBGC in the bone-implant interface suggests an increase in antigen-presenting phenotype and indicates that metal particles have more impact in the induction of immune-mediated responses. Such responses are characterized by sustained cellular hyperreactivity and phenotypic changes in Mφ subsets.
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  • 5
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Using fluorescence in situ hybridization, conventional epifluorescence microscopy, and laser scanning confocal microscopy followed by three-dimensional reconstruction we describe a well-defined higher order packaging of the human genome in the sperm cell nucleus. This was determined by the spatial localization of centromere and telomere regions of all chromosomes and supported by localization of subtelomere sequences of chromosome 3 and the entire chromosome 2. The nuclear architecture in the human sperm is characterized by the clustering of the 23 centromeres into a compact chromocenter positioned well inside the nucleus. The ends of the chromosomes are exposed to the nuclear periphery where both the subtelomere and the telomere sequences of the chromosome arms are joined into dimers. Thus chromosomes in the human sperm nucleus are looped into a hairpin-like configuration. The biological implications of this nuclear architecture in spermatogenesis and male pronuclear formation following fertilization are discussed.
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  • 6
    ISSN: 1434-6036
    Keywords: PACS. 71.30.+h Metal-insulator transitions and other electronic transitions - 75.30.Kz Magnetic phase boundaries (including magnetic transitions metamagnetion, etc.) - 75.30.Fv Spin density waves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: In order to understand the magnetic field-induced restoration of a highly conductive state in , static (SQUID) and dynamic (ESR and AFR) magnetization measurements were performed on polycrystalline samples and single crystals, respectively. In addition, cantilever and resistivity measurements under steady fields were performed. While the metal-insulator transition curve of the ( ) phase diagram exhibits a first order character, a “spin-flop” transition line divides the insulating state when the magnetic field is applied along the easy axis of magnetization. The effects of a RKKY-type indirect exchange and of applied magnetic field are described within the framework of a generalized Kondo lattice, namely two chains of localised spins coupled through the itinerant spins of the 2D sheets of BETS. The calculations, which can incorporate intramolecular electron correlations within a mean field theory, are in qualitative agreement with the field induced transition from the antiferromagnetic insulating ground state to a canted one, i.e. a not fully oriented paramagnetic, but metallic state.
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  • 7
    ISSN: 1434-6036
    Keywords: PACS. 61.66.Hq. Organic compounds[:AND:]71.18+y. Fermi surface: calculations and measurements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: The -(BETS)2C(CN)3 radical cation salt was prepared by electrocrystallization, and its crystal structure was determined by single crystal X-ray diffraction. The electronic structure of -(BETS)2C(CN)3 was studied by means of the extended Hückel tight binding method. The electrical conductivity of this salt as a function of temperature shows a metallic behaviour down to 1.3 K. Shubnikov-de Haas oscillations reveal both the classical and magnetic breakdown orbits on the Fermi surface typical of the -type organic conductors. In addition, a low frequency oscillation (250 T) which is not predicted by the band structure calculations has been found in the oscillation spectrum.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of superconductivity 12 (1999), S. 511-514 
    ISSN: 1572-9605
    Keywords: Organic superconductors ; BETS ; superconductivity ; critical field ; anisotropy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract The upper critical field B c2 of the organic superconductor λ-(BETS)2GaCl4 has been determined from resistance measurements for orientation of the magnetic field along three perpendicular crystallographic directions. The Ginzburg–Landau coherence length was estimated from the slope of B c2(T) curve near T c as 12.5 nm, 1.6 nm, and 12.5 nm for the a*-, b*-, and c-directions. Angular dependence of the critical field within highly conducting ac plane at 1.5 K was shown to possess two-fold symmetry, which can be related to the band structure anisotropy.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Advanced Materials for Optics and Electronics 8 (1998), S. 93-96 
    ISSN: 1057-9257
    Keywords: ithiolene ; tetrathiafulvalene ; molecular conductor ; molecular magnetism ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: The salt [TTF]2[Fe(tdas)2] has been prepared by electocrystallisation and its conductivity and magnetic properties are reported. The X-ray structure has been determined. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 6 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Experimental mechanics 36 (1996), S. 277-283 
    ISSN: 1741-2765
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A biaxially loaded, single edge notched (SEN) fracture specimen, with mixed modes I and II loading, was used to study the crack arrest capability of a bonded and riveted tear strap without and with simulated multiple site damage (MSD). MSD was modeled by a 50-percent groove without which the running crack would inevitably kink due to KII loading. A total of thirty-one 2024-T3 aluminum specimens with various crack and MSD configurations were tested. The fracture parameters associated with straight and curved crack paths were determined by using the experimenta results to drive a dynamic finite element model of the specimen in its generation mode. The crack kinking and extension criteria were verified by the excellent agreement between the prediction based on these fracture parameters and the measured crack kinking angles. Comparison between the test results generated by the biaxial stress specimens and by those generated by small- and full-scale pressurized fuselage rupture experiments showed that this specimen can be used to prescreen the effectiveness of tear straps and crack arrestors in an airplane fuselage.
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