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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of natural products 58 (1995), S. 68-73 
    ISSN: 1520-6025
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Twenty-eight unrelated persons heterozygous for familial hypercholesterolemia (FH) were screened to assess the frequency and nature of major structural rearrangements at the low-density lipoprotein (LDL) receptor gene in Korean FH patients. Genomic DNA was analyzed by Southern blot hybridization with probes encompassing exons 1–18 of the LDL receptor gene. Two different deletion mutations (FH29 and FH110) were detected in three FH patients (10.7%). Each of the mutations was characterized by the use of exon-specific probes and detailed restriction mapping mediated by long-PCR (polymerase chain reaction). Mutation FH29 was a 3.83-kb deletion extending from intron 6 to intron 8 and FH110 was a 5.71-kb deletion extending from intron 8 to intron 12. In FH29, the translational reading frame was preserved and the deducible result was a cysteine-rich A and B repeat truncated protein that might be unable to bind LDL but would continue to bind β-VLDL. FH110 is presumed to be a null allele, since the deletion shifts the reading frame and results in a truncated protein that terminates in exon 13. Sequence analysis revealed that both deletions have occurred between two Alu-repetitive sequences that are in the same orientation. This suggested that in these patients the deletions were caused by an unequal crossing over event following mispairing of two Alu sequences on different chromatids during meiosis. Moreover, in both deletions, the recombinations were related to an Alu sequence in intron 8 and the deletion breakpoints are found within a specific sequence, 27 bp in length. This supports the hypothesis that this region might have some intrinsic instability, and act as one of the important factors in large recombinational rearrangements.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Risk analysis 16 (1996), S. 0 
    ISSN: 1539-6924
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: In several European countries efforts are undertaken, in particular with regard to fixed industrial installations and transport of dangerous substances, to quantify the “societal risk” (SR) of accidents that may cause more than one victim at a time. This article explores the nature of such efforts. SR-models are essentially ways to structure the distribution of potential social costs of decisions about hazardous activities (e.g., costs of risk reduction, of land use forgone). First, the various ways to describe SR quantitatively, and to set limits to SR will be presented in short. Next, using a scheme developed by Fischhoff and colleagues, the various approaches will be placed in broad categories of reaching acceptable risk decisions: bootstrapping, formal analysis, and professional judgment. Each of the three categories offers a particular appreciation of the risks as ‘external costs’. This has important political implications. In the discussion it is argued that local SR-limits, by the very nature of SR, should be set in a way that creates consistency with any potential supra-local interests involved. Second, particular attention is paid to the validity of claims that SR-limits should reflect a strong risk aversion.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 66 (1995), S. 2355-2357 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: The Pohang Light Source (PLS) Project is now in its final year of the construction phase. The machine consists of a full energy (2 GeV) linear accelerator injector, a storage ring with a 12-period TBA structure, and 32 eventual beamlines from insertion devices and bending magnets. The commissioning of the injector linac was completed in June 1994. The overall commissioning of the PLS is expected to be completed by the end of 1994. The facility will provide high brightness, tunable radiation in a wide spectral range up to 30 keV of photon energy, and will serve national and international users starting in mid-1995. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 83 (1998), S. 4810-4815 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: An investigation on the effects of oxygen mixing in the sputtering gas and rapid thermal annealing on the electrical properties of radio frequency sputtered silicon oxide films has been carried out. The conductivity of the oxide film decreases as the concentration of oxygen in the sputtering gas increases, and also for rapid thermal anneal temperatures higher than 900 °C. Transport mechanisms for films annealed for 50 and 200 s were found to be that of Schottky emission and space charge limited conduction, respectively. From the capacitance versus voltage measurements, it was concluded that the improvement in the oxide quality of the annealed films was not related to the reduction of the interface trapped charge density. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 83 (1998), S. 2308-2314 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A study of the densification of radio frequency sputtered silicon oxide films by rapid thermal annealing was undertaken using both physical (etching, refractive index, and density calculation) and structural [infrared and x-ray photoelectron spectroscopy (XPS)] techniques. It was discovered that the etch rate of annealed films is reduced drastically, compared with as-deposited films and that the refractive index increases with increasing annealing temperature (Tp) or annealing time (tp). The film density also increases as Tp or tp increases and we conclude that annealed films have become denser as compared to the as-deposited films. We also suggest that increasing tp would be more efficient than raising Tp for film densification. Infrared spectrum analysis results show that with increasing Tp or tp, strain in the films has become more relaxed, and a significant amount of the surface hydroxyl groups in the annealed films was removed. The XPS analysis results show that a substantial amount of suboxide species exist within 30–40 Å of the oxide at the Si–SiO2 interface and that the Si–SiO2 interface is not abrupt. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 67 (1995), S. 1501-1502 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We demonstrate that Nd3+:ethylene glycol can be used as an amplifier gain medium for a Nd3+:YLF laser. It has been known that Nd3+:liquid is hard to use as a gain medium due to fluorescence quenching. However, we could use Nd3+:ethylene glycol as a gain medium by using a new amplification system, and we also measured the stimulated emission cross section of Nd3+:ethylene glycol. A maximum gain of ∼2 was obtained in our experimental setup, and the stimulated emission cross section of Nd3+:ethylene glycol was measured to be 1.5(±0.5) ×10−19 cm2. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York : Cambridge University Press
    Econometric theory 13 (1997), S. 606-606 
    ISSN: 0266-4666
    Source: Cambridge Journals Digital Archives
    Topics: Economics
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' journal of analytical chemistry 357 (1997), S. 32-36 
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Native β-cyclodextrin, deprotonated at its secondary hydroxyl groups has been studied as pseudostationary phase for electrokinetic chromatographic separation of highly hydrophobic compounds. Using this pseudostationary phase the separation of five polycyclic aromatic hydrocarbons (PAHs) is shown. The influence of the main parameters (field strength, pH, concentration of cyclodextrin, urea and organic solvent) on the separation of PAHs and migration behaviour of β-CD is discussed.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-203X
    Keywords: Key words Orchid ; Particle bombardment ; PLB ; Transformation ; Key terminologyProtocorm A small storage organ formed from the germinating embryo ; possessing an apical meristem and a leaf primordium ; protocorm-like body (PLB) A somatic protocorm derived from in vitro culture of apical or axillary bud meristems ; primary PLBs PLBs induced by culturing apical meristem-tips aseptically ; secondary PLBs PLBs formed on the surface of a primary PLB in culture ; proliferate PLBs PLBs proliferating on the surface of either primary or secondary PLBs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A protocol is presented for genetically engineering Cymbidium orchid using particle bombardment. This protocol enabled the routine transformation of orchid plants that were previously difficult to transform. Liquid culture was used to generate a large number of protocorm-like bodies (PLBs) to be bombarded and to promote continued development of the bombarded meristematic tissue. Plasmid DNA (pKH200) carrying the GUS-INT and NPTII genes flanked by tobacco matrix attachment regions was introduced into the meristematic cells of PLBs by particle acceleration. The transformed PLBs were proliferated and selected for kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was then induced from the kanamycin-resistant PLBs, and transgenic plantlets were produced. Both the kanamycin-resistant PLBs and regenerated shoots expressed the GUS-INT gene. The presence of the introduced gene in the transformed orchid plants was confirmed by PCR analysis, sequencing and Southern blot analysis of the PCR product. The recovered transgenic plants were established in soil and acclimatized in the greenhouse.
    Type of Medium: Electronic Resource
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