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  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 52 (1996), S. 577-578 
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Brazzein is a sweet protein isolated from a wild African plant Pentadiplandra brazzeana. Brazzein is the smallest (molecular mass = 6473 Da) and the most water-soluble protein sweetener discovered so far and is highly thermostable. Crystals were grown by vapor diffusion using sodium sulfate as a precipitant. They belong to the tetragonal space group I4122 with unit-cell parameters a = b = 61.4, c = 59.6 Å and with one molecule in the asymmetric unit. The crystals diffract to 1.8 Å resolution using synchrotron radiation.
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  • 2
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: In this study, magnetic force microscopy (MFM) was used to investigate the recording capability of a perpendicular magnetic tape at a higher linear recording density. Perpendicular magnetic tapes with polyimide base sheets 10 μm thick were fabricated by a facing-target sputtering system [H. Yoshimoto, K. Kuga, Y. Yoneda, and J. Numazawa, J. Magn. Magn. Mater. 148, 357 (1995)]. A Co–Cr–Ta (200 nm) monolayered tape with a base sheet of excellent surface roughness was fabricated. A complete recorded bit pattern of 250 kfci was observed as a MFM image on the tape. A recorded bit pattern of 300 kfci was also observed. A Co–Cr–Ta/Ni–Fe (150/50 nm) multilayered tape was also fabricated. The remnant magnetization state on this tape at 50 kfci was improved by the presence of an underlayer (Ni–Fe) only 50 nm thick. The MFM image at 250 kfci was the same as with a monolayered tape. © 1997 American Institute of Physics.
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  • 3
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 68 (1997), S. 4103-4106 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: This article reports the development of a two-dimensional analyzer, which enables us to observe the distribution of hydrogen on surfaces. A micro-focused electron beam with low primary electron energy (〈1 keV) is scanned over a sample surface, in conjunction with a time-of-flight type electron-stimulated desorption spectroscope, to obtain clear H+ ion images of a specimen surface. A line scan analysis of H+ ions on an integrated circuit and a scanning electron-stimulated desorption image of H+ ions on a Cu mesh are presented as demonstrations. © 1997 American Institute of Physics.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 54 (1998), S. 637-641 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 7 (1996), S. 317-322 
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract A non-decay type fast-setting calcium phosphate cement (nd-FSCPC) has been described, which did not decay but set within approximately 5–6 min even when the paste was immersed in serum immediately after mixing, and which forms hydroxyapatite as its end product. nd-FSCPC was produced by adding sodium alginate to the liquid phase of the base cement FSCPC. Sodium alginate forms a water-insoluble gel, and reduces the process of fluid penetration into the paste which is the cause of decay. The aim of this investigation was to confirm the mechanism of the non-decaying behaviour of nd-FSCPC proposed in a previous paper, using another chemical with properties similar to those of sodium alginate. Also, it was intended to further improve both the mechanical properties and tissue response of nd-FSCPC. Chitosan, which also forms a water-insoluble gel in the presence of calcium ions and has been reported to have pharmacologically beneficial effects on osteoconductivity, was added to the liquid phase of the base cement FSCPC. The cement thus prepared showed behaviour similar to that of nd-FSCPC using sodium alginate. The cement paste did not decay but set within approximately 5–6 min even when immersed in serum immediately after mixing. DTS value of the set mass was approximately 3–4 MPa, slightly lower than that of nd-FSCPC using sodium alginate, and no inhibitory effect was observed for the transformation of cement component to apatite within the range used in this investigation (up to 1.5%). Therefore, it was concluded that the mechanism of non-decaying behaviour was, at least in part, reduction of fluid penetration into the cement paste. nd-FSCPC using chitosan showed slightly poorer mechanical properties than that using sodium alginate. However, pharmacological effects such as osteoconductivity could be expected in nd-FSCPC using chitosan. Thus, this cement may be useful as a more sophisticated bioactive cement than nd-FSCPC using sodium alginate.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 65 (1999), S. 112-116 
    ISSN: 1432-0827
    Keywords: Key words: Radius — Metacarpus — Calcaneus — Bone mineral density — Quantitative ultrasound densitometry.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Nationwide data on bone mass were collected from 33,992 subjects who underwent screenings for osteoporosis at 65 health care centers in 11 prefectures in Japan. Reference values in Japanese women were evaluated by the four convenient methods frequently used for the screening of osteoporosis based on healthy subjects, defined by their medical history. In addition, the prevalence of low bone mass was estimated in subjects 40 years or older by the four methods. Compared with the young normal subjects aged 20–29 years old, bone mass decreased after 30, 40, and 50 year in the calcaneus by quantitative ultrasound densitometry, the radius by dual energy X-ray absorptiometry (DXA), and the metacarpus by computed X-ray densitometer and digital image processing. The prevalence of a T-score 〈−1 was relatively similar (45.6–65.5% in the fifth decade and 81.3–94.3% in sixth) in each decade among the four methods. However, the use of a T-score ≤−2.5 resulted in a marked difference in the prevalence (5.2–23.5% in the fifth decade, and 18.7–67.9% in the sixth) among the methods. The cutoff value of bone mass at a T-score −2.5 was greater than the lowest quintile value of the fifth decade in radial bone mineral density (BMD), between the lowest quintile values of the fifth and sixth decades in the two metacarpal methods, and less than the value of the sixth decade in the calcaneal Stiffness index.
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  • 7
    ISSN: 1432-203X
    Keywords: Key wordsBletilla striata ; Cryopreservation ; Embryo ; Orchid ; Vitrification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The seeds of a Japanese terrestrial orchid (Bletilla striata Rchb.f.) were germinated and cultured on solidified new Dogashima (ND) medium for 10 days. These embryos were then precultured on ND medium supplemented with 0.3 m sucrose for 3 days at 25°C in continuous dark. The embryos were then overlaid with a mixture of 2 m glycerol and 0.4 m sucrose for 15 min at 25°C and finally dehydrated with highly concentrated vitrification solution (PVS2) for 3 h at 0°C prior to immersion into liquid nitrogen for 30 min. After rapid warming, the embryos were washed with liquid ND medium supplemented with 1.2 m sucrose for 20 min and then plated on ND medium. Successfully vitrified and warmed embryos developed into normal plantlets. The rate of plant regeneration amounted to about 60%. This vitrification method appears to be a promising technique for cryopreservation of orchids.
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  • 8
    ISSN: 1432-2242
    Keywords: Wide compatibility ; Oryza sativa ; RFLP markers ; Hybrid sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RFLP analysis was conducted on a population derived from a three-way cross to determine the location of the hybrid sterility locus, S-5, in relation to mapped molecular markers and to identify markers that would be useful for selection in breeding. S-5 is of interest to rice breeders because it is associated with spikelet sterility of F1 hybrids in Indica/Japonica crosses. Identification of an S-5 allele which confers fertility in Indica/Japonica hybrids when introgressed into either the Indica or the Japonica parent has been reported. Varieties carrying this S-5 n allele are known as “wide compatibility varieties (WCV)”. Our data suggests that RFLP marker RG213 on chromosome 6 is closely linked to the S-5 locus and can be efficiently used to identify wide compatibility (WC) lines. RG213 is a single-copy genomic clone that detects three bands of different molecular weights in DNA from Japonica (‘Akihikari’) and Indica (‘IR36’) varieties and WC line (‘Nekken 2’). We demonstrate that the three alleles detected by this marker could be used to trace the inheritance of the “wide compatible” phenotype in breeders' material.
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  • 9
    ISSN: 1432-0649
    Keywords: 42.80. Jr ; 78.20. Wc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Second-Harmonic (SH) generation was observed in homeotropically aligned ferroelectric liquid-crystal cells using fundamental light propagating along the helicoidal axis. Relatively strong SH light was observed in the helicoidal structure when the optical pitch was nearly the same as the SH-light wavelength, though, otherwise, the cancellation of the generated SH light by the helicoid results in negligibly weak SH intensity. Because the observed SH light is independent of the cell thickness, the SH light is attributed to the one generated from the surface region and the SH light generated inside the cell is confined and lost by the helicoid. This phenomenon indicates the helicoidal Distributed-FeedBack (DFB) cavity action and suggests the possibility of the helicoidal DFB laser using dye-doped ferroelectric liquid-crystal cells.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 291 (1998), S. 445-454 
    ISSN: 1432-0878
    Keywords: Key words Serum-free medium ; Survival ; Chondroitin sulfate ; Culture substratum ; Brain neuron ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  When cells dissociated from the neonatal rat brains are plated on a poly-lysine-coated surface in a serum-free medium, they display a strange morphology: a dark and extended cell body. Preincubation of the surface with fetal bovine serum was found to inhibit the appearance of this strange contraction of the basal cell sheets in a dose-dependent manner. This finding indicated the presence of a factor(s) in the serum, which might be an appropriate substratum for prolonged survival of brain neurons. In the current study, this factor was highly purified through DEAE ion-exchange chromatography followed by gel filtration. The factor was eluted from a Superose column at fractions corresponding to a molecular weight greater than 1000 kDa. By SDS-PAGE analysis, these fractions were found to contain a major band (≥1000 kDa) positive for alcian blue and few minor bands faintly stainable with Coomassie blue. The activity of the purified sample, inducing the morphological change in cells, was diminished by incubation with chondroitinase ABC. Neither heparitinase II, hyaluronidase, nor trypsin modified the activity. An authentic chondroitin sulfate (type B) mimicked the serum action on the morphology of brain cells in early stages of culture. Taking these findings together, it is suggested that the factor in serum beneficial for the attachment of brain cells is composed of a chondroitin sulfate with a Mr greater than 1000 kDa. Cortical cells dissociated from the neonatal rat brain attached well to the purified factor-coated surface and displayed a healthy morphology: an optically-reflective cell body with thick neurites for at least 3 days in the absence of serum.
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