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  • 1
    Electronic Resource
    Electronic Resource
    Nucleotide sequence and presumed secondary structure of the 28S rRNA of pea aphid implication for diversification of insect rRNA (1996)
    Springer
    Journal of molecular evolution 43 (1996), S. 469-475 
    Details
    ISSN: 1432-1432
    Keywords: Aphid ; 28S rRNA ; Secondary structure model ; G + C content ; Variable region ; Divergent evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Determination of the entire nucleotide sequence of the aphid 28S ribosomal RNA gene (28S rDNA) revealed that it is 4,147 by in length with a G + C content of 60.3%. Based on the nucleotide sequence, we constructed a presumed secondary-structure model of the aphid 28S rRNA which indicated that the aphid 28S rRNA is characterized by the length and high G + C content of its variable regions. The G + C content of the aphid's variable regions was much higher than that of the entire sequence of the 28S rRNA, which formed a striking contrast to those ofDrosophila with the G + C content much lower than the entire 28S molecule. In this respect, the aphid 28S rRNA somewhat resembled those of vertebrates. This is the third report of a complete large-subunit rRNA sequence from an arthropod, and the first 28S rRNA sequence for a nondipterous insect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02337519
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  • 2
    Electronic Resource
    Electronic Resource
    Nucleotide Sequence and Presumed Secondary Structure of the 28S rRNA of Pea Aphid: Implication for Diversification of Insect rRNA (1996)
    Springer
    Journal of molecular evolution 43 (1996), S. 469-475 
    Details
    ISSN: 1432-1432
    Keywords: Key words: Aphid — 28S rRNA — Secondary structure model — G + C content — Variable region — Divergent evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Determination of the entire nucleotide sequence of the aphid 28S ribosomal RNA gene (28S rDNA) revealed that it is 4,147 bp in length with a G + C content of 60.3%. Based on the nucleotide sequence, we constructed a presumed secondary-structure model of the aphid 28S rRNA which indicated that the aphid 28S rRNA is characterized by the length and high G + C content of its variable regions. The G + C content of the aphid's variable regions was much higher than that of the entire sequence of the 28S rRNA, which formed a striking contrast to those of Drosophila with the G + C content much lower than the entire 28S molecule. In this respect, the aphid 28S rRNA somewhat resembled those of vertebrates. This is the third report of a complete large-subunit rRNA sequence from an arthropod, and the first 28S rRNA sequence for a nondipterous insect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006108
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  • 3
    Electronic Resource
    Electronic Resource
    Physical and Genetic Map of the Genome of Buchnera, the Primary Endosymbiont of the Pea Aphid Acyrthosiphon pisum (1999)
    Springer
    Journal of molecular evolution 48 (1999), S. 142-150 
    Details
    ISSN: 1432-1432
    Keywords: Key words:Buchnera—Acyrthosiphon pisum— Pea aphid — Pulse-field gel electrophoresis — Genome size — Genetic map — Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Abstract The genome of Buchnera, an endosymbiotic bacterium of the pea aphid Acyrthosiphon pisum, was characterized by pulse-field gel electrophoresis (PFGE) as a circular DNA molecule of 657 kb. The enzymes I-CeuI, CpoI, ApaI, SmaI, NaeI, SacII, MluI, FspI, and NruI were used to cleave the DNA of Buchnera into fragments of suitable size for PFGE analysis. A physical map of the Buchnera genome, including restriction fragments from seven of these enzymes, was constructed using double cutting, partial digestion, and hybridization with linking fragments, and 29 genes and operons were localized on the map. In addition, the genomic map of Buchnera was compared with those of Escherichia coli and Haemophilus influenzae. The gene order in Buchnera is more similar to that of E. coli than to H. influenzae. The dramatic shrinkage of the Buchnera genome compared with those of other members of the closely related Enterobacteriaceae family is discussed in terms of evolution under the influence of the intracellular symbiotic association.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006452
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  • 4
    Electronic Resource
    Electronic Resource
    Intracellular Bacterial Symbionts of Aphids Possess Many Genomic Copies per Bacterium (1999)
    Springer
    Journal of molecular evolution 48 (1999), S. 717-722 
    Details
    ISSN: 1432-1432
    Keywords: Key words:Buchnera— Pea aphid — Symbiosis — Genomic copy number — Dot-blot analysis — Microscope photon-counting system — Polyploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Although Buchnera, the endosymbiotic bacteria of aphids, are close relatives of Escherichia coli, their genome size is only a seventh that of E. coli. In this study, we estimated the genomic copy number of Buchnera by dot-blot hybridization and fluorimetry using a video-intensified microscope photon-counting system and obtained convincing evidence that each cell of these bacteria contains an average of 120 genomic copies. Thus, the Buchnera symbiont, with many copies of a small-sized genome, is reminiscent of cell organelles such as mitochondria and chloroplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006516
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  • 5
    Electronic Resource
    Electronic Resource
    Structure and Expression of a GroE-homologous Operon of a Macronucleus-Specific Symbiont Holospora obtusa of the Ciliate Paramecium caudatum (1998)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 45 (1998), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The reproductive form of a macronucleus-specific symbiont Holospora obtuse, when harbored by the macronucleus of the ciliate Paramecium caudatum, selectively synthesized a 63-kDa protein which is immunologically related to GroEL, or HSP60, of Escherichia coli. Heat shock treatment of isolated cells of the reproductive and infectious form of the bacterium also induced the synthesis of the GroEL homolog. Immunoblotting showed that the amount of this protein per cell, whether the reproductive or infectious form, is roughly constant. Cloning and sequencing of a gene coding for the GroEL homolog suggested that the protein is 55.2% identical to GroEL of E. coli at the amino acid sequence level, and that the gene is preceded by an open reading frame which encodes a protein 39.6% identical to GroES of E. coli. Northern blot hybridization showed that the GroEL homologous gene is highly expressed in the reproductive form, but only in a trace amount in the intermediate and infectious form. Immunoelectron microscopy revealed that the GroEL homolog is localized in the cytoplasm of the reproductive and infectious form.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1998.tb05072.x
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  • 6
    Electronic Resource
    Electronic Resource
    IN VITRO FERTILIZATION OF IN VITRO MATURED MINKE WHALE (BALAENOPTERA ACUTOROSTRATA) FOLLICULAR OOCYTES (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Marine mammal science 13 (1997), S. 0 
    Details
    ISSN: 1748-7692
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In vitro fertilization of follicular oocytes harvested from ovaries and matured in vitro was attempted for 55 minke whales (Balaenoptera acutorostrata) captured for Japanese research purposes in the Antarctic Ocean during the period from November 1995 to March 1996. In Experiment 1, effects of culture duration (96 h or 120 h) on maturation of follicular oocytes and addition of caffeine (5 mM) and/or heparin (100 pg/ml) on sperm penetration and pro-nuclear formation were investigated. Spermatozoa recovered from the vasa deferentia of four mature males were diluted (5-fold) and frozen at - 80°C. The post-thawed and pooled spermatozoa were used for in vitro insemination. A higher (P 〈 0.05) proportion of the oocytes cultured for 120 h (34.2% of 260) progressed beyond the second metaphase stage than of the oocytes cultured for 96 h (26.0% of 262). For the matured oocytes, higher rates of penetration (P 〈 0.05) and pronuclear formation (P 〈 0.01) were obtained in the oocytes cultured for 120 h (55.1% and 40.4%) than in those cultured for 96 h (32.4 % and 20.6%). Addition of caffeine and heparin did not show a significant effect. In Experiment 2, follicular oocytes matured for 120 h and then inseminated were cultured to examine the subsequent development in two culture systems (with and without co-cultured cumulus cells). Of 448 inseminated oocytes, cleaved embryos (2–16 cells) were observed with (5.8%) and without (4.9%) co-cultured systems. No cleavage was observed in 54 ova without insemination. These results indicate that in vitro fertilization of minke whale in vitro matured follicular oocytes with cryopreserved spermatozoa is possible, yielding cleaved embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1748-7692.1997.tb00647.x
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  • 7
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    Hemagglutinating properties of apolipophorin III from the hemolymph ofGalleria mellonella larvae (1998)
    Wiley
    Details
    Publication Date: 1998-01-01
    Print ISSN: 0739-4462
    Electronic ISSN: 1520-6327
    Topics: Biology
    Published by Wiley
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