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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Langmuir 11 (1995), S. 3408-3416 
    ISSN: 1520-5827
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Forest pathology 29 (1999), S. 0 
    ISSN: 1439-0329
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In order to differentiate among Phellinus pini, Inonotus tomentosus and Inonotus circinatus a polyclonal antibody was raised to a N-terminal part of 25-kDa P. pini-specific protein. The specificity of the polyclonal antibody produced against a synthetic N-terminal peptide of this protein was investigated for diagnostic purposes using Western immunoblot, indirect enzyme-linked immunosorbent assay (ELISA), and inhibition ELISA techniques. The N-terminal synthetic peptide, used as the immunogen, was found to be more than 80% pure by reverse-phase high-performance liquid chromatography. Following immunization, antisera were collected at three different time intervals. The antibody molecules were purified from the crude antisera using immunoaffinity gel chromatography. Following one-dimensional sodium dodecylsulfate-polyacrylamide gel electrophoresis, Western immunoblot analysis showed that the P. pini I polyclonal-antibody detected the immunogen, the 25-kDa protein, in all but one of the P. pini isolates examined, but in none of the isolates of the nontarget species I. tomentosus and I. circinatus. Nevertheless, cross-reactivity was a problem because the P. pini I polyclonal-antibody also recognized bands at other molecular weights in nearly all of the isolates of the other species tested. With the indirect ELISA the P. pini isolates tended to have higher affinity for the polyclonal antibody than the nontarget species, but some cross-reactivity did occur. Inhibition ELISAs, performed over a range of soluble antigen concentrations (1.56–400 ng/100 μl), failed to show a clear distinction between P. pini and the two Inonotus spp. The low level of cross-reactivity observed for I. tomentosus isolate 52 (9%) was also apparent in the indirect ELISA analysis. All three assays indicated that P. pini isolate 41 was the most antigenic. Despite cross-reactivity, the antibody is useful in Western immunoblots for the diagnosis of most P. pini isolates.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 71 (1997), S. 2361-2363 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We present the design of a novel bolometer intended for measurement of radiation within a waveguide. The thermal link in the bolometer is a low-thermal conductance micromachined beam. Power is coupled from an antenna to a resistive termination at the center of the beam. The optimization of the design for an astronomical measurement leads to an operating temperature of 0.48 K, accessible with a He3 refrigerator. Measurements on the antenna absorber in a waveguide show an average absorption of 94% from 33 to 50 GHz. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of insect behavior 8 (1995), S. 1-9 
    ISSN: 1572-8889
    Keywords: predator ; behavior ; vibrations ; searching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Observations of search behavior in the predatory stinkbugPodisus maculiventris (Say) suggested that vibrations produced by prey as they chew on leaves may be an important cue used by this predator to locate prey. To test this hypothesis, studies were conducted to determine ifP. maculiventris search and make directional choices in response to vibrational stimuli produced by feeding green cloverworms,Plathypena scabra (F.), and to recordings of chewing vibrations. Modified soybean plants [Glycine max (L.)] were used in Y-choice tests. Individuals exposed to vibrational signals finished trials significantly more often on branches through which vibrations entered the plants than on no-stimulus branches. Also, a significantly higher proportion of individuals that initially moved onto branches with no stimulus reversed course than did those moving up branches with vibrational stimuli. The response ofP. maculiventris individuals to vibrational signals produced by a common prey species demonstrates that these predators are capable of using substrate-borne vibrations as cues for prey location.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The corrosion products arising from the exposure of a Nicalon silicon carbide fibre-reinforced lithium aluminosilicate glass-ceramic matrix composite to molten sodium sulphate at 900 °C for 100 h in both oxygen and argon atmospheres were studied by X-ray diffraction (XRD) and scanning and transmission electron microscopy (SEM and TEM respectively). The microstructure of the as-received composite plates was found to be similar to that reported by other workers. The matrix consisted of grains of close to stoichiometric mullite and β-spodumene and a high silica glass with 20–50 nm wide fibre-matrix interfaces comprising a layer of turbostratic carbon and amorphous silica. The effects of hot sodium sulphate corrosion were found to be very similar in both argon and oxygen but proceeded at a much greater rate in the latter case where it had progressed 100 μm into the composite and consumed many fibres. XRD studies indicated that mullite had virtually disappeared in the corroded region and this was confirmed by SEM. TEM studies of thin sections cut from near the end of the corroded zone also showed that the matrix had become a very fine mixture of glass and β-spodumene grains and that the fibre-matrix interface region had grown to ca. 600–800 nm wide. The microstructure of this corroded interface comprised several alternating layers of turbostratic carbon, mixed carbon and amorphous silica and pure carbon, each with widths varying between ca. 100 and 200 nm. This layered structure apparently developed as a result of oxidation of the silicon carbide fibre in the presence of a gradient of oxygen partial pressure that decreased from the matrix across the interface to the fibre.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Sediment cores collected from several lakes in northern Canada have been analyzed for mercury and several other chemical contaminants. Sites ranged from the Experimental Lakes Area of northwestern Ontario, north to Cornwallis Island, and west to the southern Yukon. Cores were sliced at sites of collection and individual slices were freeze dried and analyzed for Pb-210 and Cs-137 to estimate average time intervals of deposition. The earliest date estimated by Pb-210 was about 1850, and mercury concentrations in some lakes were clearly increasing before then, assuming no vertical movements of mercury within the sediments. Extrapolation of dates downward to deeper slices, assuming a constant sedimentation rate, indicated that in some lakes mercury inputs increased slowly even in the 1500's, more rapidly after 1750, and more rapidly yet over the current century. These increases are interpreted as increased fluxes of mercury to the lakes as a result of long-range transport of atmospheric mercury, since there are no local industrial sources of mercury. Slices taken near the bottom of a core are taken to estimate the geological component while elevations in excess of that in surface slices are taken to represent contamination from fallout. This partitioning suggests that sediments in the eastern Northwest Territories are dominated by pollution, while those from the western Northwest Territories are influenced more by their geological settings. Two cores from Hudson Bay suggest that mercury is increasing there too, but has not yet exceeded geological sources. Mercury shows little or no tendency to decline in the most recent slices; indicating that inputs of mercury remain at or near their historical maxima. Given relatively high and continuing inputs of mercury to northern lakes it seems likely that some portion of that mercury may find its way into the food chain, hence the long-term prospect is for increasing levels of mercury in northern fish.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 25 (1997), S. 1119-1135 
    ISSN: 0271-2091
    Keywords: fourth-order methods ; Navier-Stokes equations ; Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A fourth-order numerical method for solving the Navier-Stokes equations in streamfunction/vorticity formulation on a two-dimensional non-uniform orthogonal grid has been tested on the fluid flow in a constricted symmetric channel. The family of grids is generated algebraically using a conformal transformation followed by a non-uniform stretching of the mesh cells in which the shape of the channel boundary can vary from a smooth constriction to one which one possesses a very sharp but smooth corner. The generality of the grids allows the use of long channels upstream and downstream as well as having a refined grid near the sharp corner. Derivatives in the governing equations are replaced by fourth-order central differences and the vorticity is eliminated, either before or after the discretization, to form a wide difference molecule for the streamfunction. Extra boundary conditions, necessary for wide-molecule methods, are supplied by a procedure proposed by Henshaw et al. The ensuing set of non-linear equations is solved using Newton iteration. Results have been obtained for Reynolds numbers up to 250 for three constrictions, the first being smooth, the second having a moderately sharp corner and the third with a very sharp corner. Estimates of the error incurred show that the results are very accurate and substantially better than those of the corresponding second-order method. The observed order of the method has been shown to be close to four, demonstrating that the method is genuinely fourth-order. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Publication Date: 1995-12-01
    Print ISSN: 0022-2461
    Electronic ISSN: 1573-4803
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Published by Springer
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  • 9
    Publication Date: 1995-09-01
    Print ISSN: 0743-7463
    Electronic ISSN: 1520-5827
    Topics: Chemistry and Pharmacology
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  • 10
    Publication Date: 1997-10-20
    Print ISSN: 0003-6951
    Electronic ISSN: 1077-3118
    Topics: Physics
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