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  • 1995-1999  (78)
Collection
Year
  • 1
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The gene designated pepR1, encoding a potential transcription regulator of Lactobacillus delbrueckii subsp. lactis DSM7290, was identified by sequence similarity of an open reading frame located upstream of the prolidase pepQ orientated in opposite direction. pepQ and pepR1 coding regions are spaced by 152 nucleotides. Upstream of the -35 region of pepQ, a 14-bp palindromic sequence, homologous to the catabolite responsive element, could be identified. The pepR1 gene has the potential to encode a protein of 333 amino acids with a calculated molecular mass of 36955 Da and a calculated p/ of 5.5. The deduced protein sequence shows significant identity to the catabolite control protein of Bacillus. Co-expression in Escherichia coli was studied with the pepR1-pepQ intergenic region fused to the promoterless β-galactosidase reporter gene. The pepQ-β-galactosidase hybrid displayed an enhanced expression in the presence of cloned pepR1.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 133 (1995), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract DNA encoding ammonia monooxygenase from two phylogenetically related autotrophic nitrifying bacteria, Nitrosospira sp. AHB1 and Nitrosolobus multiformis C-71, was amplified by PCR. The resulting products were cloned into the vector pCR-Script. A continuous region of DNA of about 1.5 kb for strain AHB1 and 1.24 kb for N. multiformis C-71 was analysed. These comprised the major part of the gene amoA encoding the active site polypeptide and, directly downstream, the 5′ portion of the amoB gene. The identity values for these sequences at the amino acid level were 93.0% for amoA and 96.1% for amoB. The corresponding values for the nucleic acid sequences were 86.7% and 88.8%, respectively. The identity of the 16S rRNA gene of strain AHB1 to that of N. multiformis C-71 was at least 98.5%. The different degree of sequence conservation between the 16S rDNA and the genes encoding for ammonia monooxygenase facilitates the application of the latter as a molecular tool for a fine-scale differentiation of autotrophic nitrifying bacteria, at the species or strain level, in both environmental and cultivation studies.
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  • 3
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To determine the role of GTP in protein targeting to the ER, we investigated GTP binding to SRP54 using crosslinking by ultraviolet light4'5 in a system consisting of [a-32P]GTP, purified SRP and purified ribosome-nascent chain complexes (RNC). The nascent chains consisted of the first 86 amino ...
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  • 4
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 68 (1997), S. 3197-3205 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: Interferometric gravitational wave detectors like VIRGO or LIGO will use mirrors suspended as pendulums, forming systems of optical cavities; this requires precise control of alignment and of the mirror pendular motions. We describe a system for the measurement of position and orientation of a mass with respect to a local reference frame. We use a single computer read out charge coupled device camera to determine all six degrees of freedom by observing reference marks on the mass, and, in the case of small mass misalignment, reflections of two auxiliary laser beams. The derived information is available in a shared memory segment on an industrial standard bus system (VME) crate for further digital processing, which will be necessary for alignment and damping of the pendular movements. The mass coordinates can be calculated at the camera frame rate of 60 Hz. In a table top experiment with fixed test mass, the short term rms measurement errors for the translational degrees of freedom were measured to be better than 0.5 μm; for two rotational degrees of freedom, the errors were better than 0.5 μrad, and for the third one about 15 μrad. The long term stability over several days is about an order of magnitude less; it is limited by drifts, to a large extent of thermal origin. © 1997 American Institute of Physics.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    International journal of earth sciences 84 (1995), S. 28-48 
    ISSN: 1437-3262
    Keywords: Norwegian ; Greenland Sea Glacial cycles ; Depositional regimes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Various models of surface and deep-water circulation in the Norwegian-Greenland Sea (NGS) have been proposed for the last two glacial to interglacial transitions. Although much progress has been made in understanding the sedimentary response to climatic and oceanographic changes, conflicting interpretations have been developed. To clarify some of these discrepancies and to test or modify the existing circulation concepts, a multiparameter approach is applied, combining sedimentological, micropaleontological, organic-geochemical and isotopic methods. On the basis of indicative properties a combined litho- and organofacies concept is developed and calibrated with modern depositional settings beneath different surface water masses. Sedimentary regimes are then derived for glacial and deglacial settings. Atlantic water intrusions in the NGS reveal complex and highly dynamic patterns for the last two glacial and interglacial periods, with repetitive inflows during Isotope Stage 6 and a high variability in Isotope Stage 5. Specific facies patterns show maximum extensions of Atlantic Water intrusions during the climatic highstands 5.5.1, 5.3 and 5.1 and narrowest intrusions in the cool phases 5.4 and most pronounced in 5.2. In contrast, different glacio-marine depositional regimes depict variable sea ice coverage and supply of ice-rafted debris. Most conspicuous are short-term depositional events marked by diamictons, which are related to the high instabilities of continental ice sheets. Some of the diamictons seem to occur contemporaneously with Heinrich layers H1 and H2. The probable temporal and obvious phenomenological concidence of Heinrich layers and NGS diamictons suggests a common trigger mechanism which caused an almost simultaneous disintegration of huge continental ice masses along the shelves of North America and the eastern margin of the NGS. A previous estuarine circulation model claims regional upwelling along the eastern margin of the NGS for specific periods of the last deglaciation. The organic character of sediments covering the same time intervals show a clear predominance of reworked fossil organic matter and thus does not support the estuarine model.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Human ecology 25 (1997), S. 319-351 
    ISSN: 1572-9915
    Keywords: commercialization ; deforestation ; sustainability ; Machiguenga ; markets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract By marshaling empirical data from five Machiguenga communities studied over 20 years, this paper disputes two common assumptions about the behavior of indigenous peoples in the face of increasing commercialization. First, many Amazonian researchers suggest that the social and ecological deterioration confronting native populations results from externally-imposed political, legal and market structures that compel local groups to pursue short-term, unstable economic strategies. Second, these structural explanations are combined with the increasing recognition that indigenous peoples possess a substantial agroecological knowledge to suggest that, if indigenous people receive control of adequate land and resources, they will implement their traditional knowledge in conservative resource management practices. In contrast to these assumptions, this analysis shows that the Machiguenga are not compelled by external forces (such as land tenure, migration policies or economic trends), but instead are active enthusiastic participants seeking to engage the market in order to acquire western goods. Further, despite highly adaptive traditional subsistence patterns and a vast agroecological knowledge, households and communities facing increasing degrees of market integration are progressively altering their traditional cropping strategies, planting practices, labor allocation and land use patterns toward a greater emphasis on commodity crop production and domesticated animal breeding. This increasing concentration on income generating activities subverts the environmentally-friendly nature of traditional productive practices and creates a socially, economically, and ecologically unsustainable system.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of intelligent and robotic systems 20 (1997), S. 45-69 
    ISSN: 1573-0409
    Keywords: robotics ; autonomous systems ; parallel algorithms ; parallelism and concurrency ; motion planning ; review
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract One of the many features needed to support the activities of autonomoussystems is the ability to plan motion. This enables robots to move in theirenvironment securely and to accomplish given tasks. Unfortunately, thecontrol loop comprising sensing, planning, and acting has not yet beenclosed for robots in dynamic environments. One reason involves the longexecution times of the motion planning component. A solution for thisproblem is offered by the use of highly parallel computation. Thus, animportant task is the parallelization of existing motion planning algorithmsfor robots so that they are suitable for highly parallel computation. Inseveral cases, completely new algorithms have to be designed, so that aparallelization is feasible. In this survey, we review recent approaches tomotion planning using parallel computation. As a classification scheme, weuse the structure given by the different approaches to the robot’smotion planning. For each approach, the available parallel processingmethods are discussed. Each approach is assigned a unique class. Finally,for each research work referenced, a list of keywords is given.
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  • 8
    ISSN: 1573-5028
    Keywords: epidermis specifcity ; promoter comparison ; RNA polymerase processivity ; transgenic rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The contribution of sequences upstream and downstream of the transcription start site to the strength and specificity of the promoter of rice tungro bacilliform virus was analysed in transgenic rice plants. The promoter is strongly stimulated by downstream sequences which include an intron and is active in all vascular and epidermal cells. Expression in the vascular tissue requires a promoter element located between −100 and −164 to which protein(s) from rice nuclear extracts bind. Elimination of this region leads to specificity for the epidermis. Due to the presence of a polyadenylation signal in the intron, short-stop RNA is produced from the promoter in addition to full-length primary transcript and its spliced derivatives. The ratio between short-stop RNA and full-length or spliced RNA is determined by upstream promoter sequences, suggesting the assembly of RNA polymerase complexes with different processivity on this promoter.
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  • 9
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1997 (1997), S. 887-891 
    ISSN: 0947-3440
    Keywords: Heck reaction, intramolecular ; Isochromane ; Palladium ; Heterocycles ; High-pressure chemistry ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The iodoarenes 9 and 10, bearing (E)- and (Z)-alkene moieties, respectively, easily prepared by alkylation of the corresponding alcohols 7a-c and 8a-c with 2 iodobenzyl iodide, undergo intramolecular Heck reactions to give the isochromanes 12-15a-c in good yields. The selectivity of the reaction depends on the size of the substituent R in 9 and 10; increasing bulkiness of R leads to a decrease in diastereoselectivity and an increase in regioselectivity. High-pressure experiments confirm the proposed mechanism and show that bromoarenes such as 11, which tend to be unreactive at ambient pressure, give good results when the cyclisation is performed under high pressure.
    Additional Material: 1 Tab.
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  • 10
    ISSN: 0947-3440
    Keywords: Heck reaction, intramolecular ; α-Amino acids ; Isoquinolines ; Palladium ; Heterocycles ; Synthesis, enantioselective ; High pressure ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Alkylation and acylation of the alkenes 9b, c and 10a, b, which are easily prepared from the corresponding α-amino alcohols, with 2-halobenzyl and 2-halobenzoyl halides respectively, gives 11-15. These compounds cyclize with excellent diastereo- and regioselectivity to the enantiomerically pure N-heterocycles 18-20 in an intramolecular Heck reaction using 5 mol-% of Pd(OAc)2 in the presence of PPh3, TPAB and KOAc. Under the same conditions substrate 17 leads to the enantiomerically pure benzazepine 24. The reaction of the bromoarene derivatives 14-15 must be performed under high pressure to give good results.
    Additional Material: 1 Tab.
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