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  • 1
    Electronic Resource
    Electronic Resource
    .beta.-D-Cellotetraose Hemihydrate as a Structural Model for Cellulose II. An X-ray Diffraction Study (1995)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 117 (1995), S. 11397-11406 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00151a003
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a cosmid contig for the GCPS gene region (1995)
    Springer
    Human genetics 〈Berlin〉 95 (1995), S. 82-88 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The zinc finger gene GLI3 has been shown to be involved in the embryonal development of the limbs and skull. Mutations in GLI3 lead to the development of the human Greig cephalopolysyndactyly syndrome (GCPS) and the mouse mutations extra toes (Xt) and anterior digit deformity (add). The GCPS locus on human chromosome 7p13 has recently been isolated in a yeast artifical chromosome (YAC) contig. Here, we describe the establishment of a cosmid contig that was derived from two of the YAC clones, that spans 550 kb of human DNA, and that includes the GLI3 gene. In this contig, three GCPS translocation breakpoints have been mapped to distinct EcoRI fragments in the 3′ half of the gene. In addition, exon-carrying fragments have been identified and the size of the GLI3 gene could be determined as at least 280 kb. The gene is flanked by a CpG island that lies on the 5′ side and that is in close proximity to the first exon detected by the cloned GLI3 cDNA. Further upstream, five segments were found that have been conserved between man and mouse. In the mouse, this region has been characterized as the transgene integration site resulting in the add phenotype. Both the CpG island and the conserved regions are probable candidates for a search for GLI3 promoter and control elements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225080
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  • 3
    Electronic Resource
    Electronic Resource
    Six new polymorphic microsatellite markers used for the integration of genetic and physical maps of human chromosome 7 (1996)
    Springer
    Human genetics 〈Berlin〉 97 (1996), S. 842-844 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We report the isolation and characterization of six new polymorphic dinucleotide repeat microsatellite markers (D7S1491, D7S1492, D7S1493, D7S1494, D7S1495, and D7S1496), their integration into the genetic map of human chromosome 7 by analysis of 40 CEPH (Centre d’Etude du Polymorphisme Humain) pedigrees, and their use for integration of physical and genetic maps of this chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050148
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  • 4
    Electronic Resource
    Electronic Resource
    Six new polymorphic microsatellite markers used for the integration of genetic and physical maps of human chromosome 7 (1996)
    Springer
    Human genetics 〈Berlin〉 97 (1996), S. 842-844 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We report the isolation and characterization of six new polymorphic dinucleotide repeat microsatellite markers (D7S1491, D7S1492, D7S1493, D7S1494, D7S1495, and D7S 1496), their integration into the genetic map of human chromosome 7 by analysis of 40 CEPH (Centre d'Etude du Polymorphisme Humain) pedigrees, and their use for integration of physical and genetic maps of this chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02346201
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  • 5
    Electronic Resource
    Electronic Resource
    Development of reliable PCR markers for the selection of the Vf gene conferring scab resistance in apple (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 118 (1999), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Early selection of scab-resistant apple seedlings can be enhanced by the use of markers tightly linked to the Vf resistance gene. Two sequence characterized amplified regions (SCAR) markers have been obtained from previously described random amplified polymorphic DNA (RAPD) markers. AM19-SCAR is a codominant marker, while AM19-SCAR is dominant, as is the RAPD from which it was derived. A highly detailed map in the vicinity of the Vf gene was built through the cumulative analysis of about 600 seedlings from six different controlled crosses. The usefulness of these and other SCAR markers will be discussed in relation to combining the traditional phenotypic selection with MAS. The availability of two codominant, tightly linked markers flanking both sides of the resistance gene (AL07-SCAR and M18-CAPS) also makes it easy to identify the seedlings homozygous for the resistance gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.1999.118002183.x
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  • 6
    Electronic Resource
    Electronic Resource
    Production and purification of Clostridium botulinum type C and D neurotoxin (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 24 (1999), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Neurotoxins of Clostridium botulinum are needed in basic neurologic research, but as therapeutic agent for certain neuro-muscular disorders like strabism as well. A method for the production and purification of botulinum neurotoxins C and D is reported using a two-step hollow-fiber cross flow filtration and a newly developed chromatographic purification procedure. Hollow-fiber filtration proved to be a rapid and safe concentration and pre-purification step, which can easily be scaled up. The chromatographic purification included hydrophobic interaction, anion exchange and size exclusion chromatography runs. Botulinum neurotoxins C and D could be recovered with an overall yield of 12.6% and 10.6%, respectively. A specific toxicity of 1.86×107 minimal lethal dose mg−1 (type C) and 5.26×107 minimal lethal dose mg−1 (type D) was determined in the mouse bioassay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-695X.1999.tb01306.x
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  • 7
    Electronic Resource
    Electronic Resource
    Inorganic-Organic Polymers with Barrier Properties for Water Vapor, Oxygen and Flavors (1998)
    Springer
    Journal of sol gel science and technology 13 (1998), S. 141-146 
    Details
    ISSN: 1573-4846
    Keywords: inorganic-organic polymers ; barrier coating ; barrier adhesive ; laminating agent ; water vapor barrier ; oxygen barrier ; flavor barrier
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract With a new kind of barrier coating material, inorganic-organic polymers, it is possible to obtain high-barrier properties with respect to the permeation rates of oxygen, water vapor and volatile organic compounds. The hybrid polymers are accessible via the sol-gel technique. The inorganic network is formed as a result of controlled hydrolysis and condensation of alkoxysilanes, organoalkoxysilanes, acryloxysilanes or metal alcoholates. The organic network results from subsequent thermal or UV-induced polymerization of organo-functional groups. Due to the control of the inorganic and organic network densities and the insertion of specific functional groups to control the polarity of the resulting material, it was possible to develop high-barrier coatings with excellent adhesion properties on a wide variety of polymer films. These high-barrier coatings are also suitable as adhesives which can be used in laminates. The properties of the processable multilayer structures are preserved to a much higher extent than with other comparable, commercially available materials even under high mechanical and thermal stress and storage in humidity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008628029870
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  • 8
    Electronic Resource
    Electronic Resource
    Construction of a 550 kb BAC contig spanning the genomic region containing the apple scab resistance gene Vf (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 884-891 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsVf ; Malus×domestica ; Resistance gene ; Chromosome walking ; Inverse PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A positional cloning project was started in apple with the aim of isolating the Vf resistance gene of Malus floribunda 821. Vf confers resistance against apple scab, the most important disease in apple orchards. A chromosome walk starting from two molecular markers (M18-CAPS and AM19-SCAR) flanking Vf was performed, using a bacterial artificial chromosome (BAC) library containing inserts of the cultivar Florina, which is heterozygous for Vf. Thirteen BAC clones spanning the region between the two markers were identified in nine chromosome walking steps. The size of the resulting contig is approximately 550 kb. In order to map the Vf region in more detail, we analyzed over 2000 plants from different populations segregating for Vf with markers produced from BAC end sequences. In this way, we were able to restrict the possible location of the Vf gene to a minimum of five clones spanning an interval of approximately 350 kb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051154
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  • 9
    Electronic Resource
    Electronic Resource
    Towards the map-based cloning of Vf: fine and physical mapping of the Vf Region (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 1012-1017 
    Details
    ISSN: 1432-2242
    Keywords: Key words Malus ×domestica ; Venturia inaequalis ; Malus floribunda ; Disease resistance ; Genetic map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A map-based cloning scheme is being used to isolate the Vf resistance gene of apple against the fungus Venturia inaequalis. Vf is a major dominant gene that is inherited in a Mendelian manner and influenced by minor genes that modify its activity. The two recently published markers M18 and AL07, bracketing Vf, were tested on 1179 progeny plants of three crosses to fine-map Vf. M18 and AL07 were positioned at 0.2 cM and 1.1 cM from Vf respectively, for a total distance between the two markers of 1.3 cM. Physical mapping by pulsed-field gel electrophoresis, using M18 and AL07 as probes, demonstrated that both markers hybridize to a common 870 kb NotI restriction fragment. We therefore found a relationship between physical and genetic distance of 670 kb/cM in the Vf region. This led us to the conclusion that a chromosome walk using the recently published apple BAC library starting from M18 and AL07 is feasible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051409
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  • 10
    Electronic Resource
    Electronic Resource
    Molecular selection in apple for resistance to scab caused by Venturia inaequalis (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 199-204 
    Details
    ISSN: 1432-2242
    Keywords: Malus ; Molecular markers ; Apple scab ; Plant breeding ; Marker assisted selection ; Venturia inaequalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Large-scale marker-assisted selection requires highly reproducible, consistent and simple markers. The use of genetic markers is important in woody plant breeding in general, and in apple in particular, because of the high level of heterozygosity present in Malus species. We present here the transformation of two RAPD markers, which we found previously to be linked to the major scab resistance gene Vf, into more reliable and reproducible markers that can be applied directly to apple breeding. We give an example of how the use of such markers can speed up selection for the introduction of scab resistance genes into the same plant, reducing labour and avoiding time-consuming test crosses. We discuss the nature and relationship of the scab resistance gene Vf to the one present in Nova Easygro, thought to be Vr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225746
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