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  • Wiley-Blackwell  (22)
  • American Geophysical Union (AGU)
  • 1995-1999  (22)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Production and characterization of a plant α-hydroxynitrile lyase in Escherichia coli (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 53 (1997), S. 332-338 
    Details
    ISSN: 0006-3592
    Keywords: α-hydroxynitrile lyase ; cassava ; cyanogenesis ; cyanohydrin ; Escherichia coli expression vector ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The coding sequence of the cyanogenic α-hydroxynitrile lyase gene of Manihot esculenta Crantz (cassava) was cloned in the plasmid vector pMal-c2 and expressed in Escherichia coli strain JM105. DNA sequencing showed that the recombinant plasmid contained the same sequence as the cDNA clone pHNL10. Peptide sequencing of the recombinant protein showed that the N-terminus was heterogeneous, with either four or six additional amino acid residues compared with the native protein. Circular dichroism spectra indicated similar secondary structure contents for both proteins. Enzyme assays showed that specific activity of native and recombinant proteins were 0.24 and 0.26 mmol CN-/mg/min, respectively; that both proteins had optimal activity at 40°C and pH 5.5; and that both proteins were inhibited by the serine protease inhibitor phenyl-methane sulfonyl flouride (PMSF). Isoelectric focusing of native and recombinant protein revealed multiple isoforms for both proteins; the recombinant protein had a more basic mean isoelectric point (pl) (5.1) than the native protein (4.5). © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 332-338, 1997.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Protein-nucleic acid interactions and DNA conformation in a complex of human immunodeficiency virus type 1 reverse transcriptase with a double-stranded DNA template-primer (1997)
    New York : Wiley-Blackwell
    Biopolymers 44 (1997), S. 125-138 
    Details
    ISSN: 0006-3525
    Keywords: AIDS ; DNA structure ; polymerase structure ; protein - DNA interaction ; x-ray crystallography ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The conformation of the DNA and the interactions of the nucleic acid with the protein in a complex of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) and a 19-mer/18-mer double-stranded DNA template-primer (dsDNA) are described. The structure of this HIV-1 RT complex with dsDNA serves as a useful paradigm for studying aspects of nucleotide polymerases such as catalysis, fidelity, drug inhibition, and drug resistance. The bound dsDNA has a bend of approximately 41° at the junction of an A-form region (first five base pairs near the polymerase active site) and a B-form region (the last nine base pairs toward the RNase H active site). The 41° bend occurs smoothly over the four base pairs between the A-form portion and the B-form portion in the vicinity of helices αH and αI of the p66 thumb subdomain. The interactions between the dsDNA and protein primarily involve the sugar - phosphate backbone of the nucleic acid and structural elements of the palm, thumb, and RNase H of p66, and are not sequence specific. Amino acid residues from the polymerase active site region, including amino acid residues of the conserved Tyr-Met-Asp-Asp (YMDD) motif and the “primer grip,” interact with 3′-terminal nucleotides of the primer strand and are involved in positioning the primer terminal nucleotide and its 3′-OH group at the polymerase active site. Amino acid residues of the “template grip” have close contacts with the template strand and aid in positioning the template strand near the polymerase active site. Helix αH of the p66 thumb is partly inserted into the minor groove of the dsDNA and helix αI is directly adjacent to the backbone of the template strand. Amino acid residues of Β1′, αA′, αB′, and the loop containing His539 of the RNase H domain interact with the primer strand of the dsDNA. © 1997 John Wiley & Sons, Inc. Biopoly 44: 125-138, 1997
    Additional Material: 8 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    Immunogold labeling of oncogenic and tumor related proteins (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 31 (1995), S. 159-173 
    Details
    ISSN: 1059-910X
    Keywords: Immunogold ; Electron microscopy (EM) ; Oncogene ; Mos ; Met ; Ski ; Muc1 ; Mucin ; Immunocytochemistry ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Immunogold labeling electron microscopy technique has been used to study the ultrastructural localization of oncogenic proteins: Mos, Met, Ski, and the tumor-associated protein, Muc1, as well as their relationship with other tumor-related proteins. By pre- and postembedding immunogold labeling electron microscopy techniques, we showed that the Mos protein pp39mos colocalized with microtubule bundles, suggesting that microtubulin or microtubule-associated protein(s) may be the substrate of Mos. Met protein was labeled at the microvilli of the lumen that are formed in cultured T47D cells, implying its potential involvement in lumen formation. Ski localization experiments revealed a unique globular structure “Ski body” that is present inside the nucleus of interphase chicken embryo fibroblast infected with Ski cDNA FB29 and FB2-29. Ski bodies were also found scattered in the cytoplasm of metaphase FB29 and FB2-29 Ski expressing chicken embryo fibroblasts. In T47D cells, tumor-associated protein Muc1 was associated with both the plasma membrane and the membranes of secretory vesicles in the cytoplasm. In MUC1 infected NIH3T3 cells, however, labeling showed that in addition to the plasma membrane and the membranes of secretory vesicles, some Muc1 gold spheres were seen inside the secretory vesicles, suggesting that the subcellular localization of the protein may vary in different cell types. © 1995 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070310207
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  • 4
    Electronic Resource
    Electronic Resource
    Biodegradation of phenol using the self-cycling fermentation (SCF) process (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 51 (1996), S. 112-119 
    Details
    ISSN: 0006-3592
    Keywords: biodegradation ; self-cycling fermentation ; phenol ; Pseudomonas putida ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Self-cycling fermentation (SCF) in a stirred tank reactor was applied to the biodegradation of phenol by Pseudomonas putida. The technique resulted in stable and repeatable performance. Complete substrate consumption was achieved under all operating conditions investigated. SCF resulted in substrate utilization rates as high as 14.5 kg of phenol per cubic meter of fermentor volume per day of fermentation, higher than those that have been reported for batch, CSTR, and packed column fermentors. A mathematical model of the self-cycling fermentation process was expanded to include inhibitory substrate-microorganism combinations, and was shown to provide a good fit to both end-of-cycle and intracycle experimental data. © 1996 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    Rapid spectroscopic confirmation of the presence of polystyrene in polymer particles of mixed composition (1998)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 36 (1998), S. 999-1004 
    Details
    ISSN: 0887-6266
    Keywords: latex ; polystyrene ; luminescence ; particle ; micron-diameter ; Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: A technique for rapid determination of the presence of polystyrene in individual micron-diameter polymer particles of mixed composition is presented. This technique is based upon observation of visible emission from conjugated regions of the polymer backbone, generated photochemically, while the particle is held in an optical trap. Particle emission characteristics are dependent upon particle size and suspending solvent. Emission spectra are provided for single component polystyrene particles and mixed polymer particles containing poly(methyl methacrylate), poly(N-vinyl pyrrolidone), and polystyrene. © 1998 John Wiley & Sons, Inc. J Polym Sci B: Polym Phys 36: 999-1004, 1998
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Catalytic transformation of copaiba (Copaifera officinalis) oil over zeolite ZSM-5 (1995)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 18 (1995), S. 54-58 
    Details
    ISSN: 0935-6304
    Keywords: High resolution capillary GC ; High resolution GC-MS ; Zeolite ZSM-5 ; Copaiba tree (Copaifera officinalis) ; Copaiba oil ; Fuel oil ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The oil extracted from the trunk of the copaiba tree (Copaifera officinalis) is composed of sesquiterpenes, C15H24, and a small amount (〉7 %) of sesquiterpenols, C15H26O; these were identified from their Kováts indices and mass spectra. The use of zeolites in the catalytic transformation of this renewable source of hydrocarbons is of interest in the search for new chemicals and ecologically clean fuels. Oil samples from copaiba trees growing in Colombia's Oriental Plains were circulated over zeolite ZSM-5 in a continuous flow fixed-bed micro reactor at 225, 265, and 325°C, for 1 h and subsequently analyzed by HRGC and GC-MS. Whereas only thirty four sesquiterpenes were identified in the original oil, over two hundred compounds were found in the product of the treatment of copaiba oil with zeolite ZSM-5. This complex mixture of sesquiterpenes, light aromatic compounds, and indene and naphthalene derivatives resulted from reactions such as isomerization, hydrogenation, cracking, and dehydrogenation with and without cracking. The amount of cracking products and aromatic compounds increased with reactor temperature but decreased with catalyst aging.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240180112
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  • 7
    Electronic Resource
    Electronic Resource
    XPS and SEM characterization of hydrated cerium oxide conversion coatings (1995)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 23 (1995), S. 540-550 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Cerium-rich conversion coatings have been deposited onto aluminium 2024-T351 alloy by immersion into a solution containing 10 g L-1 CeCl3 and 1% H2O2 in a process described as ‘cerating’. Prior to deposition the alloy had been prepared either by using a standard chemical pretreatment used for aerospace alloys before conversion coating or by polishing. X-ray photoelectron spectroscopy and scanning electron microscopy have been used to characterize these cerium-containing conversion coatings. It was found that, during deposition, hydrated cerium oxide initially covered the intermetallics present in the alloy surface and then covered the surface generally. Deposition continued over the intermetallics throughout the conversion coating process, resulting in thick, heavily-cracked regions considerably greater than the average thickness of the film (〉0.3 μm). Elsewhere the coating was generally up to 0.2 μm thick, and appeared to comprised of deposited particles around 100 nm in size.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740230714
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  • 8
    Electronic Resource
    Electronic Resource
    Unusual Peak Shifts in the Core Levels of CeO2 Films Deposited on Si(100) (1996)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 24 (1996), S. 634-640 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: X-ray photoelectron spectroscopy has been used to study 20, 100 and 1000 nm evaporated cerium oxide films on Si(100) single-crystal wafers. Upon exposure to the x-ray source there was loss of oxygen and generation of Ce3+. Furthermore, for the 20 nm coating, there was evidence of a shift to higher binding energies of the C 1s peak and a high binding energy O 1s component relative to the oxygen anion peak at 529.7±0.1 eV with increased exposure time to the x-ray source. No similar shift was observed in the O KLL Auger lines, suggesting that the effect was not due to differential charging of the surface with respect to the bulk of the coating. Hence the relative shift is explained in terms of electronic effects resulting from the formation of anion vacancies in the surface. These include a shift of the Fermi level due to defect states in the bandgap as well as band bending due to the positive charge set up from the anion vacancies.
    Additional Material: 8 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    Chromate Conversion Coatings on 2024 Al Alloy (1997)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 25 (1997), S. 223-234 
    Details
    ISSN: 0142-2421
    Keywords: conversion coating ; aluminium ; alloy ; x-ray photoelectron spectroscopy ; scanning electron microscopy ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and potentiodynamic measurements have been made on chromate conversion-coated Al 2024-T3 alloy. X-ray photoelectron spectroscopy measurements indicated that the conversion coating had a surface of CrOOH and Cr(VI), enriched in ferricyanide. The bulk of the coating was an equal mixture of CrOOH and Cr2O3 with significant levels of F- and Fe, the latter implying the presence of ferricyanide throughout the coating. Copper(II) ion was present at the interface between the conversion coating and the alloy, as well as Al3+. During ageing experiments, potentiodynamic measurements indicated that the corrosion current (icorr) decreased from ∽0.4 to ∽0.04 μA cm-2 during the first 40 h after preparation but thereafter slowly increased. No significant changes were observed in the chemistry of the coating by XPS for ageing times longer than 40 h, although morphological changes were observed with SEM. As the coating aged, a network of microcracks developed across the surface. It is believed that Cr6+ is consumed in the process in which plugs of hydrated chromium oxide form at the base of these cracks. © 1997 by John Wiley & Sons, Ltd.
    Additional Material: 11 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    A case study in parallel computation: Viscous flow around an ONERA M6 wing (1995)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 21 (1995), S. 877-884 
    Details
    ISSN: 0271-2091
    Keywords: ONERA M6 wing ; parallel computing ; viscous flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: We examine the solution of a practical engineering problem on a parallel computer. The problem involves the steady laminar viscous flow about an ONERA M6 wing and the computer is a 64-processing-node Connection Machine CM-5E. We show that efficient domain decomposition procedures lead to a balanced load on the processors and low communication times. The net result is that solutions can be attained in roughly 20 min elapsed time for a 48,011-node, 266,566-element unstructured mesh. We conclude that this is sufficiently fast to support the design process.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650211008
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