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  • 1995-1999  (40)
  • 1980-1984  (22)
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  • 1
    Electronic Resource
    Electronic Resource
    Complexation of zinc tetraphenylporphyrin by adsorbed poly(4-vinylpyridine): equilibrium studies (1983)
    s.l. : American Chemical Society
    Inorganic chemistry 22 (1983), S. 3050-3053 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00163a014
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  • 2
    Electronic Resource
    Electronic Resource
    Synthesis and chemistry of 9-cyclohexyl-5(7)-(dimethyl sulfide)-nido-decaborane(11), B10H11C6H11S(CH3)2 (1980)
    s.l. : American Chemical Society
    Inorganic chemistry 19 (1980), S. 1182-1187 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic50207a014
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  • 3
    Electronic Resource
    Electronic Resource
    Inward rectifier potassium channels in plants differ from their animal counterparts in response to voltage and channel modulators (1995)
    Springer
    European biophysics journal 24 (1995), S. 107-115 
    Details
    ISSN: 1432-1017
    Keywords: Potassium channel ; KAT1 ; Voltage dependence ; Cesium block ; pH dependence ; Kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract We have investigated the electrophysiological basis of potassium inward rectification of the KAT1 gene product from Arabidopsis thaliana expressed in Xenopus oocytes and of functionally related K+ channels in the plasma membrane of guard and root cells from Vicia faba and Zea mays. The whole-cell currents passed by these channels activate, following steps to membrane potentials more negative than −100 mV, with half activation times of tens of milliseconds. This voltage dependence was unaffected by the removal of cytoplasmic magnesium. Consequently, unlike inward rectifier channels of animals, inward rectification of plant potassium channels is an intrinsic property of the channel protein itself. We also found that the activation kinetics of KAT1 were modulated by external pH. Decreasing the pH in the range 8.5 to 4.5 hastened activation and shifted the steady state activation curve by 19 mV per pH unit. This indicates that the activity of these K+ channels and the activity of the plasma membrane H+-ATPase may not only be coordinated by membrane potential but also by pH. The instantaneous current-voltage relationship, on the other hand, did not depend on pH, indicating that H+ do not block the channel. In addition to sensitivity towards protons, the channels showed a high affinity voltage dependent block in the presence of cesium, but were less sensitive to barium. Recordings from membrane patches of KAT1 injected oocytes in symmetric, Mg2+-free, 100 mM-K+, solutions allowed measurements of the current-voltage relation of single open KAT1 channels with a unitary conductance of 5 pS. We conclude that the inward rectification of the currents mediated by the KAT1 gene product, or the related endogenous channels of plant cells, results from voltage-modulated structural changes within the channel proteins. The voltage-sensing or the gating-structures appear to interact with a titratable acidic residue exposed to the extracellular medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00211406
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  • 4
    Electronic Resource
    Electronic Resource
    The Endocrine Responses to Protein Calorie Malnutrition (1983)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Nutrition 3 (1983), S. 187-212 
    Details
    ISSN: 0199-9885
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.nu.03.070183.001155
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  • 5
    Electronic Resource
    Electronic Resource
    New procedures for preparation of potassium 3-aminopropylamide (1984)
    s.l. : American Chemical Society
    The @journal of organic chemistry 49 (1984), S. 2494-2496 
    Details
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jo00187a038
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  • 6
    Electronic Resource
    Electronic Resource
    2-Phenyl-4-hydroxyphthalazin-1-one: A Benzoannelated Derivative of Maleic Hydrazide (1998)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 54 (1998), S. 671-676 
    Details
    ISSN: 1600-5740
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The monoclinic crystals (space group P21/a, Z = 8) of 2-phenyl-4-hydroxyphthalazin-1-one, C14H10N2O2, have two independent molecules (A and B) in the asymmetric unit. Both occur as the lactim–lactam (hydroxy–one) structure, which is also found in the parent molecule maleic hydrazide (both triclinic and monoclinic polymorphs), dichloromaleic hydrazide and luminol (3-aminophthalhydrazide). The molecular arrangement is based on strings of alternating A and B molecules linked by hydroxyl...carbonyl hydrogen bonds, with only van der Waals interactions between adjacent strings. Comparison is made of the measured bond lengths for (monoclinic) maleic hydrazide and values from high-level ab initio calculations, and reasonably good agreement is obtained, with indications of improvements when allowance is made for electron correlation and hydrogen bonding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108768197015760
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  • 7
    Electronic Resource
    Electronic Resource
    Efficient transformation of scutellar tissue of immature maize embryos (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 737-748 
    Details
    ISSN: 1432-2242
    Keywords: Key words Biolistic transformation ; Maize ; Cereals ; Somatic embryogenesis ; Immature embryos
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   An efficient transformation system for maize was established by improving transformation conditions for the particle bombardment of the scutellar tissue of immature embryos. Particle bombardment was carried out using constructs containing the pat gene as the selection marker and a PDS 1000/He gun (Biorad). Transformation parameters, such as the amount of gold particles used per bombardment, particle velocity, preculture time of the scutellum prior to bombardment and osmotic treatment of the target tissue before and after bombardment, were analysed. Fertile transgenic regenerants of the maize inbred lines H99, A188 and Pa91 and the crosses A188×H99 and Pa91×H99 were selected on Basta-containing medium. The transformation frequency was between 2% and 4%. A total of 29 transgenic plant lines was obtained and verified with Southern blot analysis. All of the transgenic plants were fertile and set seeds. The R1 progeny of single plants was analysed. A Mendelian segregation of the transgenes was observed for all of the transformants tested. For 1 candidate, stable inheritance and stable expression of the transgenes were followed up to the R4 generation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050473
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  • 8
    Electronic Resource
    Electronic Resource
    Localization of introduced genes on the chromosomes of transgenic barley, wheat and triticale by fluorescence in situ hybridization (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 749-757 
    Details
    ISSN: 1432-2242
    Keywords: Key words Transgenic barley ; wheat and triticale ; Chromosomal localization ; FISH ; Integration pattern
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Using fluorescence in situ hybridization (FISH) we localized introduced genes on metaphase chromosomes of barley, wheat, and triticale transformed by microprojectile bombardment of microspores and scutellar tissue with the pDB1 plasmid containing the uidA and bar genes. Thirteen integration sites were detected in the nine lines analysed. Southern analysis showed that three or more copies of the plasmid were present in the lines. In a triticale line containing four copies three different integration sites were identified, indicating that the method described is sensitive enough for the detection of single-copy integrations. There was a slight tendency towards the localization of transgenes in distal chromosome regions. Using the GAA-satellite sequence for chromosome banding, the chromosomes containing the inserted genes were identified in most cases. Two barley lines derived from the same transformant showed a totally different integration pattern. Southern analysis confirmed that the inserted genes were segregating independently, resulting in different integration patterns among the progeny lines. The application of the FISH technique for the analysis of transgenic plants is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050474
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  • 9
    Electronic Resource
    Electronic Resource
    Diagnostic and therapeutic applications of azulenyl nitrone spin traps (1999)
    Springer
    Cellular and molecular life sciences 56 (1999), S. 626-633 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Azulenyl nitrones; azulenes; nitrones AZN; PBN; S-PBN; free radicals; spin traps; antioxidants; neuroprotection; oxidative stress; assay; amyotrophic lateral sclerosis; FALS; superoxide dismutase; transgenic mice; stroke; ischemia/reperfusion; Parkinson’s disease; MPTP; axotomy; neurodegeneration.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Azulenyl nitrones have been recently demonstrated to constitute a new class of nitrone-based spin traps with the unprecedented capacity to tag free radicals by yielding characteristically colored and highly visible diamagnetic (and paramagnetic) spin adducts. In addition, a comparison of the oxidation potentials of azulenyl nitrones such as 1 and congeners to those of conventional nitrone spin traps previously investigated as potential antioxidant therapeutics such as N-tert-butyl-α-phenylnitrone and its related ortho-sodium sulfonate reveals that the azulene-derived spin traps are far more readily oxidized. These special features render azulenyl nitrones of interest with regard to both their distinct ability to engender the convenient use of colorimetric detection to monitor free radical-mediated oxidative stress in biological systems, and to their potentially enhanced efficacy as neuroprotective antioxidants vs. those conventional nitrone spin traps earlier examined as such. Herein is reported an overview of recent developments pertaining to the use of azulenyl nitrones in the detection of oxidative stress in animal models of amyotrophic lateral sclerosis and stroke, and to their neuroprotective activity in animal models of Parkinson’s disease, stroke and neurodegeneration within the retina.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050457
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  • 10
    Electronic Resource
    Electronic Resource
    Degradation of 4-chloro-2-methylphenol by an activated sludge isolate and its taxonomic description (1995)
    Springer
    Biodegradation 6 (1995), S. 83-92 
    Details
    ISSN: 1572-9729
    Keywords: Biodegradation ; 2-methyl-4-carboxymethylenebut-2-en-4-olide ; 4-chloro-2-methylphenol ; taxonomy ; waste water treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The Gram-negative strain S1, isolated from activated sludge, metabolized 4-chloro-2-methylphenol by an inducible pathway via a modifiedortho-cleavage route as indicated by a transiently secreted intermediate, identified as 2-methyl-4-carboxymethylenebut-2-en-4-olide by gas chromatography/mass spectrometry. Beside 4-chloro-2-methylphenol only 2,4-dichlorophenol and 4-chlorophenol were totally degraded, without an accumulation of intermediates. The chlorinated phenols tested induced activities of 2,4-dichlorophenol hydroxylase and catechol 1,2-dioxygenase type II. Phenol itself appeared to be degraded more efficiently via a separate, inducibleortho-cleavage pathway. The strain was characterized with respect to its physiological and chemotaxonomic properties. The fatty acid profile, the presence of spermidine as main polyamine, and of ubiquinone Q-10 allowed the allocation of the strain into the α-2 subclass of theProteobacteria. Ochrobactrum anthropi was indicated by fatty acid analysis as the most similar organism, however, differences in a number of physiological features (e.g. absence of nitrate reduction) and pattern of soluble proteins distinguished strain S1 from this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00695339
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