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  • Oxford University Press  (277)
  • Blackwell Publishing Ltd
  • Nature Publishing Group
  • 2010-2014  (243)
  • 1995-1999  (92)
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  • 1
    Electronic Resource
    Electronic Resource
    In Vitro Excystation of Spironucleus muris (1996)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 43 (1996), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . In vitro excystation of Spironucleus muris cysts, purified by sequential sucrose and Percoll gradients from mouse feces, was studied. Three in vitro excystation procedures, used for Giardia, were assessed to determine the most useful method. Excystation was monitored by light microscopy and subsequently characterized by transmission and scanning electron microscopy. Spironucleus muris excysted routinely at a level greater than 90% when induced in Hanks’ balanced salt solution containing sodium bicarbonate at pH 2.0 and transferred to Tyrodes’ salt solution as an excystation medium. Similarly, high rates of excystation were recorded after induction of S. muris cysts in 0.1 M potassium phosphate buffer (pH 7.0) with sodium bicarbonate and excystation in trypticase-yeast extract-iron medium (TYI medium) or phosphate-buffered saline. A lower rate and percentage of excystation were observed after induction of S. muris cysts in an aqueous hydrochloric acid solution (pH 2.0) followed by excystation in TYI medium. All excystation methods produced extremely active S. muris trophozoites with normal morphology. Nonexcysting S. muris cysts have a wall composed of an outer fibrous and an inner membranous portion. Following induction, numerous vesicles appeared in the peritrophic space. Excystation began by the cyst wall opening at one pole, and the anterior part of the trophozoite protruding from the cyst wall. The trophozoite emerged progressively from the cyst wall and the empty cyst wall appeared to collapse. Excysted trophozoites exhibited normal morphological features of S. muris trophozoites isolated from the mouse intestine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1996.tb02473.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genome plasticity in Neisseria gonorrhoeae (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 145 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The pathogenic Neisseria have exploited the processes of horizontal DNA transfer and genetic recombination as mechanisms for the generation of extensive protein variation and modulation of gene expression. Localized recombinations have been well documented in members of multigene families as have alterations in short repetitive sequences. Here we report an analysis of the chromosomal structure of a defined lineage of Neisseria gonorrhoeae strain MS 11 pilin variants. This study reveals the occurrence of large rearrangements, including the amplification of a 26 kb region and an inversion involving more than a third of the chromosome. Additionally, a restriction site polymorphism that correlates with pilin expression has been observed. These findings highlight the flexibility of the gonococcal genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08574.x
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  • 3
    Electronic Resource
    Electronic Resource
    Sequential action of factors involved in natural competence for transformation of Neisseria gonorrhoeae (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 137 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract We previously identified and genetically characterized several factors essential for the natural competence of transformation in Neisseria gonorrhoeae. Here we analyse the sequential action of these factors and dissect the overall transformation process into three distinct steps, (i) the sequence-specific uptake of transforming DNA into a DNase-resistant state, (ii) the transfer of DNA to the cytosol and (iii) the processing and recombination of the incoming with the resident DNA. While two pilus-associated factors, PilE and PilC, were previously implicated in the early DNA uptake event, we show here that three competence factors unrelated to pilus biogenesis, ComA, ComL and Tpc, are not essential for DNA uptake and rather act in a subsequent step. The respective mutants, however, lack the characteristic nucleolytic processing observed with the incoming DNA in both wild-type and non-transformable RecA-deficient N. gonorrhoeae, indicating that they are blocked in the processing and/or the delivery of DNA to the cytoplasm. A hypothetical model proposing a sequential action of the known gonococcal competence factors is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08099.x
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of hydrogenase activities associated with the molybdenum CO dehydrogenase from Oligotropha carboxidovorans (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 136 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The formation of H2 by chemolithoautrophically growing Oligotropha carboxidovorans has been identified as the result of the oxidation of CO mediated by the cytoplasmic species of the molybdenum-containing CO dehydrogenase multienzyme complex as follows: CO+H2O→CO2+H2. Purified CO dehydrogenase was shown to carry hydrogen uptake and formation activities in addition to its catabolic function which is the oxidation of CO. Among the electron donors supporting H2 formation were CO, NADH, reduced flavins and reduced viologen dyes. The reduction of protons to H2 by cytoplasmic CO dehydrogenase is interpreted as a detoxification reaction for electrons to prevent cell damage in O. carboxidovorans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08042.x
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  • 5
    Electronic Resource
    Electronic Resource
    C-terminal glycine-histidine tagging of the outer membrane protein Iga β of Neisseria gonorrhoeae (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 127 (1995), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A glycine-histidine tag (Gly3His6) was added to the C-terminus of a fusion protein consisting of the cholera toxin B-subunit (CtxB) and the IgA protease β-domain (Iga β). The aim was to facilitate single-step purification and to create a suitable tool for kinetic and structural studies on Iga β-driven protein translocation across the outer membrane of Gram-negative bacteria. We demonstrate that the glycine-histidine tag does not interfere with the assembly of Iga β in the outer membrane and that the translocator function of the modified Iga β is maintained. The applicability of the new construct for the dissection of the Iga β mediated translocation process and general aspects of C-terminal histidine tagging of outer membrane proteins are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07481.x
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  • 6
    Electronic Resource
    Electronic Resource
    High-molecular-mass, iron-repressed cytoplasmic proteins in fluorescent Pseudomonas: potential peptide-synthetases for pyoverdine biosynthesis (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 132 (1995), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract High molecular-mass cytoplasmic proteins were detected in iron-starved, pyoverdine-producing Pseudomonas aeruginosa, P. chlororaphis, P. Fluorescens, P. putida, P. aptata and P. tolaasii. specifically located in the cytoplasm and thus were termed ‘IRCPs’, for iron-repressed cytoplasmic proteins. A strain-dependent gel electrophoresis pattern with multiple bands of Mr values ranging from 180 to 600 kDa was usually observed for these proteins. Strains synthesizing pyoverdines differing in their peptide part presented different IRCP gel electrophoresis profiles, whereas strains synthesizing identical pyoverdines had identical IRCP gel electrophoresis profiles. Some mutants affected in pyoverdine biosynthesis presented a perturbed IRCP pattern, and no IRCPs were detected in non-fluorescent Pseudomonas strains either unable to synthesize siderophores or synthesizing non-peptidic siderophores. The data strongly suggest that the IRCPs could be related to peptide synthetases involved in the biosynthesis of the peptidic part of pyoverdine-type siderophores.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07803.x
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  • 7
    Electronic Resource
    Electronic Resource
    Basic Aspects of Vasorelaxant and Bronchodilating Peptides in Clinical Use: Urodilatin (INN: Ularitide), VIP, and PACAP (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 805 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb17503.x
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  • 8
    Electronic Resource
    Electronic Resource
    The Effects of Bag Type and Meter Size on Seepage Meter Measurements (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 37 (1999), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Seepage meters provide a useful, inexpensive method of measuring seepage flux through unconsolidated sediments in lakes, reservoirs, and stream channels. In the literature, the bag type used is usually not indicated, and its effect on seepage measurement has not been evaluated. The effects of bag type and seepage meter size have been evaluated in a laboratory setting.A sandbox 100.4 (L) × 76.2 (W) × 24 (H) cm size was half filled with coarse sand and water to a depth of 10 cm. Four seepage meters and two bag types (balloon and condom) were used in the seepage measurements.There was no significant difference between the seepage measurements obtained from the two bag types. A two-sample t-test (df=56, Wt-sat=2.58, alpha=0.01, p=0.0013) failed to reject the null hypothesis of similarity between measurements from the two bag types. The seepage measurements from four seepage meters were similar with no significant difference; however, the smaller diameter seepage meters had a greater variance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1999.tb01119.x
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  • 9
    Electronic Resource
    Electronic Resource
    Oral Tolerance in Experimental Autoimmune Encephalomyelitis (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 778 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb21130.x
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  • 10
    Electronic Resource
    Electronic Resource
    Oral Tolerance in Myelin Basic Protein TCR Transgenic Mice (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 778 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb21159.x
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