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  • 2000-2004  (744)
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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning and characterization of a chitosanase from the chitosanolytic bacterium Burkholderia gladioli strain CHB101 (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 354-360 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A chitosanase was purified from the culture fluid of the chitino- and chitosanolytic bacterium Burkholderia gladioli strain CHB101. The purified enzyme (chitosanase A) had a molecular mass of 28 kDa, and catalyzed the endo-type cleavage of chitosans having a low degree of acetylation (0–30%). The enzyme hydrolyzed glucosamine oligomers larger than a pentamer, but did not exhibit any activity toward N-acetyl-glucosamine oligomers and colloidal chitin. The gene coding for chitosanase A (csnA) was isolated and its nucleotide sequence determined. B. gladioli csnA has an ORF encoding a polypeptide of 355 amino acid residues. Analysis of the N-terminal amino acid sequence of the purified chitosanase A and comparison with that deduced from the csnA ORF suggests post-translational processing of a putative signal peptide and a possible substrate-binding domain. The deduced amino acid sequence corresponding to the mature protein showed 80% similarity to the sequences reported from Bacillus circulans strain MH-K1 and Bacillus ehimensis strain EAG1, which belong to family 46 glycosyl hydrolases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530000388
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  • 2
    Electronic Resource
    Electronic Resource
    Relativistic electronic structure calculations on endohedral Gd@C60, La@C60, Gd@C74, and La@C74 (2000)
    Springer
    Applied physics 70 (2000), S. 461-464 
    Details
    ISSN: 1432-0630
    Keywords: PACS: 61.48.+c; 31.15. Ew
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract. Relativistic discrete-variational local density functional calculations on endohedral Gd@C60, La@C60,Gd@C74, and La@C74 are performed. All the C60- and C74-derivedlevels are lowered upon endohedral Gd and La doping. Both the Gd (4f75d16s2) and La (5d16s2) atoms only donate their two 6s valence electrons to the cages, leaving behind their 5d electrons when they are placed at the cage centers. Compared with large-band-gap C60, small-band-gap C74 and Gd (La)-metallofullerenes have strong both electron-donating and electron-accepting characters, and the calculated ionization potentials and electron affinities for them agree well with the available experimental data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003390051068
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  • 3
    Electronic Resource
    Electronic Resource
    Garment's pressure sensation (1): subjective assessment and predictability for the sensation (2002)
    Bingley : Emerald
    International journal of clothing science & technology 14 (2002), S. 307-316 
    Details
    ISSN: 0955-6222
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In order to study the wearing comfort of pressure for tight-fit clothing, the sensation of wearing pressure and the other related sensations have been assessed for knit garments, which have different sizes and fabrics which have different extensibilities, by developing a wearing experimental procedure. Using factor analysis with principal factor solutions and rotated by the Varimax method, we obtained relevant factor matrices about the subjective assessment. At the same time, objective clothing pressure, fabric extensibility and garment fitness have been measured. Regression analysis showed that the garment fitness and fabric extensibility had great predictive power for the subjective pressure assessment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/09556220210446121
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  • 4
    Electronic Resource
    Electronic Resource
    Garment's pressure sensation (2): the psychophysical mechanism for the sensation (2002)
    Bingley : Emerald
    International journal of clothing science & technology 14 (2002), S. 317-327 
    Details
    ISSN: 0955-6222
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Relationships have been investigated between subjective pressure sensation and objective pressure measured, for knit garments of different sizes and fabrics with different extensibilities. Fechner's logarithmic law is used to investigate the relations. Equations are obtained for describing the Psychophysical mechanism of clothing pressure perception under certain conditions. Objective pressure measuring had high predictive power with regard to subjective pressure sensation only under those conditions. Wearing pressure comfort has a negative correlation with feelings of fetter, scratchy, heavy and pressure, and has a poor correlation with feelings of softness and smoothness. Using factor analysis with principal factor solutions and rotated by the Varimax method, we obtained factor matrices.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/09556220210446248
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  • 5
    Electronic Resource
    Electronic Resource
    Mössbauer studies of melt-spun Pr2Fe14B ribbons (2001)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 90 (2001), S. 2033-2035 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Melt-spun ribbons of tetragonal Pr2Fe14B exhibit favorable hard magnet characteristics. Technically relevant materials based on this compound, however, generally contain a certain amount of soft magnetic α-Fe or Fe3−xBx for remanence enhancement through exchange coupling. The nominal off-stoichiometric compositions lead to metallurgical complications, which are not easily resolvable by standard phase identification techniques such as x-ray diffraction and thermal magnetic analysis. As a viable alternative, 57Fe-Mössbauer spectroscopy can be used to delineate individual Fe sites. To provide a basis for such an approach, this report gives Mössbauer parameters including hyperfine magnetic field, isomer shift, and quadrupole splitting as obtained from a single-phase melt-spun Pr2Fe14B ribbon. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1385353
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  • 6
    Electronic Resource
    Electronic Resource
    Epitaxial NiO hillocks on truncated octahedral nanoparticles of passivated Ni (2001)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 89 (2001), S. 3061-3063 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Epitaxial NiO hillocks on the {111} and {001} facets of truncated octahedral nanoparticles of Ni have been directly observed by high-resolution transmission electron microscopy. These nanometer hillocks form a rough shell enclosing the Ni nanoparticle. The epitaxial relationships of NiO on nanoparticles of Ni are the same as those of NiO on bulk Ni {111} and {001} surfaces. The formation of hillocks is related to the relaxation of the compressive stress in NiO arising from the very large lattice mismatch between NiO and Ni. The compressively stressed epitaxial NiO shell provides effective protection to the nanoparticles of Ni against further oxidation. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1345856
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  • 7
    Electronic Resource
    Electronic Resource
    Head and backbone of the Early Cambrian vertebrate Haikouichthys (2003)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 421 (2003), S. 526-529 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Agnathan fish hold a key position in vertebrate evolution, especially regarding the origin of the head and neural-crest-derived tissue. In contrast to amphioxus, lampreys and other vertebrates possess a complex brain and placodes that contribute to well-developed eyes, as well as auditory and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature01264
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  • 8
    Electronic Resource
    Electronic Resource
    Primitive deuterostomes from the Chengjiang Lagerstätte (Lower Cambrian, China) (2001)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 414 (2001), S. 419-424 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Cambrian fossil-Lagerstätten (sites of exceptional fossil preservation), such as those from Chengjiang (Lower Cambrian) and the Burgess Shale (Middle Cambrian), provide our best window into the Cambrian ‘explosion’. Such faunas are known from about 40 localities, and have yielded a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/35106514
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  • 9
    Electronic Resource
    Electronic Resource
    DNA sequence and comparative analysis of chimpanzee chromosome 22 (2004)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 429 (2004), S. 382-388 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Human–chimpanzee comparative genome research is essential for narrowing down genetic changes involved in the acquisition of unique human features, such as highly developed cognitive functions, bipedalism or the use of complex language. Here, we report the high-quality DNA sequence of 33.3 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature02564
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  • 10
    Electronic Resource
    Electronic Resource
    An Early Cambrian tunicate from China (2001)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 411 (2001), S. 472-473 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Like the Burgess Shales of Canada, the Chengjiang Lagerstätte from the Lower Cambrian of China is renowned for the detailed preservation as fossils of delicate, soft-bodied creatures, providing an insight into the Cambrian explosion. The fossils of possible hemichordate chordates and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/35078069
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