ISSN:
1617-4623
Keywords:
Translation initiation Chlamydomonas reinhardtii Chloroplast
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. The chloroplast atpB gene of Chlamydomonas reinhardtii, which encodes the β subunit of the ATP synthase, contains three in-frame ATGs that are candidate translation initiation codons. An earlier study revealed that the N terminus of the assembled β subunit maps at the +2 position with respect to the second in-frame methionine codon (Fiedler et al. 1995). Using chloroplast transformation, we have examined the possibility that either of the two additional in-frame ATG codons is competent for translation initiation. We provide evidence that translation of atpB is initiated exclusively at the second ATG codon. We conclude that the β subunit is not synthesized with an N-terminal leader before its assembly into a functional ATP synthase complex.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380000332
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