ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 2000-2004  (2)
Collection
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sclerotinia sclerotiorum is considered the most devastating pathogen of sunflower grown in humid environments. In this study, progenies of partial hybrids between Helianthus maximiliani, a wild species that has been shown to be resistant to S. sclerotiorum, and H. annuus were characterized by amplified fragment length polymorphism (AFLP) analysis to identify whether there are introgressions from H. maximiliani into the cultivated sunflower at the molecular level. Wild species-specific fragments as well as fragments not found in either parent were detected. Progenies tended to cluster together according to the original partial hybrids in the dendrogram by the use of bootstrap procedures. The progenies were studied for their reaction to S. sclerotiorum using artificial inoculation of sunflower heads. Some of the progenies showed a higher level of resistance compared with resistant inbred lines. It was possible to identify two AFLP-fragments which seem to be linked to Sclerotinia resistance.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 101 (2000), S. 1250-1258 
    ISSN: 1432-2242
    Keywords: Key words Micronuclei ; Microprotoplasts ; Chromosome transfer ; RAPD ; Helianthus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Asymmetric somatic hybrid (ASH) plants were obtained by PEG-mediated mass fusion of microprotoplasts from perennial Helianthus species and hypocotyl protoplasts of Helianthus annuus. The formation of micronuclei in perennial sunflower cell cultures was induced, at early log phase, by addition of the herbicides amiprophos-methyl or oryzalin. Sub-diploid microprotoplasts were isolated by high-speed centrifugation and the smallest enriched by sequential filtration through nylon sieves of decreasing pore size. Fusion products were cultured and the regenerated plants phenotypically, genetically and cytologically characterized. DNA analysis using RAPD markers revealed that 28 out of 53 regenerated plants were asymmetric hybrids. Subsequent nuclear-DNA flow cytometric analysis showed that these plants had a higher DNA content than the receptor H. annuus, suggesting that they represented addition lines. Cytological investigation of the metaphase cells of 16 hybrids revealed an addition of 2–8 extra chromosomes in these plants. The phenotype of most ASH plants resembled H. annuus. These results indicate that micronuclear induction and asymmetric somatic hybridization represent a potent tool for partial genome transfer aimed at the specific transfer of economically important traits in breeding programs.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...