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  • 1
    Electronic Resource
    Electronic Resource
    Construction of a genetic linkage map for witloof (Cichorium intybus L. var. foliosum Hegi) (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A genetic linkage map based on an intraspecific cross between two inbred lines of witloof-chicory (Cichorium intybus L. var. foliosum Hegi) has been constructed. In total, 129 RAPD markers were scored in 565 F2 plants. Grouping of these markers at a LOD of threshold 4.0 resulted in nine linkage groups, which is equal to the chicory haploid genome. The nine linkage groups covered 609.6 cM. All 129 RAPD markers were linked to one of the nine groups. Three RAPD markers could not be mapped. Out of the 126 remaining RAPD markers, 18 showed segregation distortion with significance value of P 〈 0.01.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2003.00869.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of commercial chicory cultivars for hydroponic forcing and their phenetic relationships revealed by random amplified polymorphic DNAs and amplified fragment length polymorphisms (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: One-hundred and twenty-four amplified fragment length polymorphism (AFLP) and 49 random amplified polymorphic DNA (RAPD) markers have been used to distinguish between 20 and 23 commercial chicory cultivars, respectively. These were all Cichorium intybus var. foliosum F1 hybrids, currently used in hydroponic forcing. Five-hundred and twenty RAPD primers (OPERON) were tested, of which 156 resulted in reproducible patterns and 26 yielded polymorphisms. Two-hundred and fifty-six AFLP primer-combinations were tested and six combinations were selected for identification purposes. Similarity indices were measured and clustering has been done using pairwise comparison. Both types of marker provide similar conclusions. Two major clusters are formed, representing late and early cultivars. All cultivars were identified using 10 informative RAPD primers or three AFLP primer combinations. A low degree of polymorphism was detected between some early cultivars, suggesting a narrow genetic base in their breeding strategy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00499.x
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  • 3
    Electronic Resource
    Electronic Resource
    The phylogenetic relationship between different Cichorium intybus cultivars and cultivar groups, as revealed by RAPDs (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The phylogenetic relationship between different Cichorium intybus cultivars and cultivar groups was studied. A total of 127 random amplified polymorphic DNA markers have been used to distinguish between 51 Cichorium intybus cultivars belonging to three different cultivar groups. A principal component analysis was performed on the complete data set and a clear grouping of chicory cultivar groups was observed. The three chicory groups tested (root chicory, witloof and pain de sucre) were separated from one another in the principal component plots. The first two axes accounted for 43.9% of the total variation. Although the chicory used in traditional forcing in soil and the red chicory cultivars were related more to each other and to the chicory cultivars from the witloof group, they still formed separate groups in the principal component plot.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00633.x
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  • 4
    Electronic Resource
    Electronic Resource
    Cryopreservation of in vitro sugar beet (Beta vulgaris L.) shoot tips by a vitrification technique (2000)
    Springer
    Plant cell reports 19 (2000), S. 1064-1068 
    Details
    ISSN: 1432-203X
    Keywords: Key words Beta vulgaris L. Cryopreservation ; Shoot tips ; Encapsulation-dehydration ; Vitrification ; AbbreviationsBA: N-6-benzylaminopurine ; CMS:¶Cytoplasmic male sterile ; DMSO: Dimethylsulfoxide ; DGJ: Medium of De Greef and Jacobs (1979) ; DGJ0: Medium of De Greef and Jacobs (1979) lacking BA ; IBA: Indole-3-butyric acid ; kin: 6-Furfurylaminopurine ; LS: Loading solution ; PAR: Photosynthetic active radiation ; PVS2: Plant vitrification solution ; RS: Recovery solution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Sugar beet shoot tips from cold-acclimated plants were successfully cryopreserved using a vitrification technique. Dissected shoot tips were precultured for 1 day at 5  °C on solidified DGJ0 medium with 0.3 M sucrose. After loading for 20 min with a mixture of 2 M glycerol and 0.4 M sucrose (20  °C), shoot tips were dehydrated with PVS2 (0  °C) for 20 min prior to immersion in liquid nitrogen. Both cold acclimation and loading enhanced the dehydration tolerance of shoot tips to PVS2. After thawing, shoot tips were deloaded for 15 min in liquid DGJ0 medium with 1.2 M sucrose (20  °C). The optimal exposure time to both loading solution and PVS2 depended on the in vitro morphology of the clone. With tetraploid clones a higher sucrose concentration during cold acclimation and preculture further enhanced survival after cryopreservation. Survival rates ranged between 60% and 100% depending on the clone. Since only 10–50% of the surviving shoot tips developed into non-hyperhydric shoots, regrowth was optimized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000232
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  • 5
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    Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress (2001)
    EMBO Press
    Details
    Publication Date: 2001-03-01
    Print ISSN: 0261-4189
    Electronic ISSN: 1460-2075
    Topics: Biology , Medicine
    Published by EMBO Press on behalf of European Molecular Biology Organization.
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