ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The ability of soluble fibers to inhibit calf pregastric lipase (CPGL)-catalyzed hydrolysis of tributyrin (TBG) has been measured at pH 6.5, 37 °C (CPGL 0.02 mg/mL). Fibers tested were pectin, carboxymethylcellulose (CMC), carrageenan, and gum arabic. The fibers (5 g/L), solubilized in Bis-Tris buffer (50 mM, pH 6.5) at 4 °C for either 15 min or 24 h, were added to an emulsion containing TBG, before initiating the reaction by addition of CPGL. An immediate inhibitory effect of 8% to 19% was observed. The value of the CPGL–pectin dissociation constant, Ki, was 0.76 to 2.34 mM. Dialysis of pectin solution reduced the inhibitory effect by 14% and the concentration of galacturonic acid by 15%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.2003.tb08294.x
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