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  • 1
    Electronic Resource
    Electronic Resource
    Introgression of genes into cultivated Brassica napus through resynthesis of B. napus via ovule culture and the accompanying change in fatty acid composition (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Fifteen lines of Brassica napus were resynthesized via ovule culture through 24 interspecific crosses between four Brassica oleracea and three Brassica campestris accessions. The degree of success in the interspecific crosses was significantly influenced by maternal genotypes. The interspecific hybrid production rate (HPR) varied with combinations from 0 to 76.9%, with a mean HPR of 24.7% for the crosses with B. campestris as the female parent and 6.9% for the crosses with B. oleracea as female parent. Twenty-four crosses between seven natural and six resynthesized B. napus gave, on average, 10.3 seeds per pod, and ranged from 1.2 to 22.0 seeds per pod, depending on genotypes of both parents. Resynthesized lines of B. napus showed high erucic acid content and variable content of linolenic acid, ranging from 3.4% to 9.9%. The fatty acid composition in hybrid seeds between natural and resynthesized B. napus was dominated by the embryo genotypes; an additive mode was shown for erucic acid and positive over-dominance for linolenic acid content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00641.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic analysis of petal size through genomic manipulation in Brassica (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effect of genome composition and cytoplasm on petal size was studied in Brassica. Two accessions of Brassica rapa (2n = 20, AA) were reciprocally crossed with three accessions of Brassica oleracea (2n =18, CC) to produce resynthesized B. napus (2n = 38, AACC or CCAA) and sesquidiploids (2n = 29, AAC or CAA). Petal size was measured and compared among diploids (AA and CC), sesquidiploids (AAC and CAA) and amphidiploids(AACC and CCAA). The results showed that petal size is a genome-dependent and highly heritable character. The heritability of petal length is as high as 96.3%. The addition of each C-genome to the AA genomic background increased the petal length by 4-5 mm. Cytoplasm of B. oleracea showed a positive effect on petal length by about 1.3 mm over that of B. rapa. Petal width was positively correlated with petal length at a highly significant level (r= 0.806, df = 81). Resynthesized B. napus (AACC) showed significantly larger flower petals than natural rapeseed cultivars (AACC).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.00992.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of genome composition and cytoplasm on petal colour in resynthesized amphidiploids and sesquidiploids derived from crosses between Brassica rapa and Brassica oleracea (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effect of genome composition and cytoplasm on petal colour was studied in Brassica. Three accessions of yellow-petalled B. rapa (2n= 20, AA) were crossed with a white-petalled B. oleracea var. alboglabra (2n= 18, CC) and with three cream-yellow-petalled B. oleracea var. gongylodes (2n= 18, CC) to produce resynthesized B. napus (2n= 38, AACC or CCAA) and sesquidiploids (2n= 29, AAC or CAA). Petal colour was measured with a Hunter automatic colour difference meter. The results revealed that petal colour in Brassica is controlled by nuclear genes and by cytoplasmic factors. Additive and epistatic gene effects were involved in the action of nuclear genes. When crosses were made between yellow-petalled B. rapa and white-petalled B. oleracea var. alboglabra, significant additive, epistatic and cytoplasmic effects were found. White petal colour was partially epistatic over yellow petal colour. When crosses were made between yellow-petalled B. rapa and cream-yellow-petalled B. oleracea var. gongylodes, only epistatic effects were detected. Yellow petal colour was epistatic over cream-yellow.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2002.722295.x
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  • 4
    Electronic Resource
    Electronic Resource
    Various population fluctuation patterns of light-attracted beetles in a tropical lowland dipterocarp forest in Sarawak (2000)
    Springer
    Population ecology 42 (2000), S. 97-104 
    Details
    ISSN: 1437-5613
    Keywords: Key words General flowering ; Light-trap ; Population dynamics ; Scarabaeidae ; Meloidae ; Borneo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The population fluctuation pattern of light-attracted beetles was studied from August 1992 to September 1998 (for 73 months) using ultraviolet light-traps set at three vertical levels in a tropical lowland dipterocarp forest in Sarawak, Malaysia. During our study, a general flowering occurred from April to July in 1996, and flowering on a small scale in 1997 and 1998. We analyzed the data for eight scarabaeid and six meloid species, some of which were anthophilous species. Various fluctuation patterns were observed among the beetle species in aspects of both seasonality and correlation with the supraannual phenological pattern. Three large chafer species (Scarabaeidae, Melolonthini) showed a clear seasonal fluctuation pattern with a peak once from March to May every year, the peak monthly catch greatly fluctuating annually. Other scarabaeid beetles did not show such a clear seasonal population pattern and hardly fluctuated annually. Populations of an anthophilous scarabaeid species, Parastasia bimaculata, a specific pollinator of Homalomena propinqua (Araceae), hardly fluctuated, probably because of its response to the constant flowering of its floral hosts. Monthly catches of an anthophilous scarabaeid, Anomala sp., and meloid beetles showed clear supraannual patterns in response to the general flowering and were significantly correlated with the flowering intensity with or without a lag of a month. The fluctuation pattern of meloids suggests a supraannual population fluctuation pattern of their hosts, i.e., megachilid/anthophorid bees.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101440050014
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  • 5
    Electronic Resource
    Electronic Resource
    A novel nuclear factor, SREB, binds to a cis-acting element, SRE, required for inducible expression of the Aspergillus oryzae Taka-amylase A gene in A. nidulans (2000)
    Springer
    Molecular genetics and genomics 263 (2000), S. 232-238 
    Details
    ISSN: 1617-4623
    Keywords: Key words Taka-amylase A gene ; Aspergillus nidulans ; Gene regulation ; Nuclear factor ; Cis-acting element
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Taka-amylase A gene (taaG2) of Aspergillus oryzae is inducibly expressed in A. nidulans upon exposure to inducing carbon sources, such as starch and maltose. In order to identify nuclear factor(s) possibly involved in the induction of the taaG2 gene, gel mobility shift assays and DNase I footprinting analyses were carried out, and revealed a novel nuclear factor in A. nidulans extracts, which specifically bound to two sites in the taaG2 promoter region, −204 to −189 and −182 to −168, which share the common sequence GGAAATT. The nuclear factor was detected in nuclei from both induced and uninduced mycelia. Mutational analysis within and around the binding sequences demonstrated that only the upstream binding sequence, designated SRE (starch responsive element), was required for the inducible expression of the taaG2 gene, and thus we designated the nuclear factor SREB (SRE binding factor). The downstream binding site contained an inverted SRE (ISRE) and played no role in the induction of taaG2 expression. SREB was shown by gel retardation assays to have higher affinity for SRE than for ISRE.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051164
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  • 6
    Electronic Resource
    Electronic Resource
    A New Centerless Grinding Technique without Employing a Regulating Wheel (2003)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 238-239 (Apr. 2003), p. 355-362 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/46/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.238-239.355.pdf
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  • 7
    Electronic Resource
    Electronic Resource
    Determination of an Optimum Geometrical Arrangement of Workpiece in the Ultrasonic Elliptic-Vibration Shoe Centerless Grinding (2004)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 257-258 (Feb. 2004), p. 495-500 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/47/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.257-258.495.pdf
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  • 8
    Electronic Resource
    Electronic Resource
    Modeling of Grinding Force in Constant-Depth-of-Cut Ultrasonically Assisted Grinding (2004)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 471-472 (Dec. 2004), p. 101-106 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper discusses the mechanism behind the grinding force decrease associated with ultrasonication of the grinding wheel in constant-depth-of-cut ultrasonically assisted grinding (UAG). By introducing a grinding model describing the cutting trace of an abrasive grain, an equation relating the grindingforce decrease to such process parameters as the amplitude and frequency of vibration and the grinding wheel speed, is established. Experiments are conducted to confirm the theoretical prediction. Theoretical and empirical results both indicate that the decrease in grinding force is due to the grinding chips becoming smaller and fracturing more easily under ultrasonication. The results also suggest that the grinding force decrease is greater at higher vibration amplitudes and at lower grinding wheel speeds
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/09/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.471-472.101.pdf
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  • 9
    Electronic Resource
    Electronic Resource
    Identification of Rad51 alteration in patients with bilateral breast cancer (2000)
    Springer
    Journal of human genetics 45 (2000), S. 133-137 
    Details
    ISSN: 1435-232X
    Keywords: Key wordsRad51 ; Hereditary breast cancer ; BRCA1 ; BRCA2 ; Germline alteration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The human Rad51 gene, HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair. BRCA1 and BRCA2 proteins form a complex with Rad51, and these genes are thought to participate in a common DNA damage response path-way associated with the activation of homologous recombination and double-strand break repair. Additionally, we have shown that the pattern of northern blot analysis of the Rad51 gene is closely similar to those of the BRCA1 and BRCA2 genes. It is therefore possible that alterations of the Rad51 gene may be involved in the development of hereditary breast cancer. To investigate this possibility, we screened Japanese patients with hereditary breast cancer for Rad51 mutations and found a single alteration in exon 6. This was determined to be present in the germline in two patients with bilateral breast cancer, one with synchronous bilateral breast cancer and the other with synchronous bilateral multiple breast cancer. In both patients, blood DNAs showed a G-to-A transition in the second nucleotide of codon 150, which results in the substitution of glutamine for arginine. As this alteration was not present in any patients with breast or colon cancer examined, we assume that this missense alteration is likely to be a disease-causing mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380050199
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  • 10
    Electronic Resource
    Electronic Resource
    Expression of SAG-1 of Toxoplasma gondii in transgenic mice (2000)
    Springer
    Parasitology research 86 (2000), S. 263-269 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We describe the expression of SAG-1 cDNA in B6C3F1 mice by microinjecting a 3.3 kbp DNA fragment, consisting of the cytomegalovirus enhancer-chicken β-actin hybrid promoter and SAG-1 into the pronucleus of a fertilized egg at the one-cell stage. Offspring derived from this microinjection were analyzed for the integration and functional expression of the SAG-1 transgene. Steady-state expressions of both the mRNA for SAG-1 and SAG-1 protein product were detected in the brain, thymus, spleen and liver. Approximately 50% of F1 and F2 progeny inherited the SAG-1 transgene from SAG-1 transgenic mice in Mendelian fashion. These results indicated that SAG-1 transgenic lines were established. Transgenic mice harboring the SAG-1 gene will contribute a critical tool of defining the molecular mechanisms of SAG-1 in pathogenesis and host immune response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004360050041
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