ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
`Squid-type' diisopropylfluorophosphatases (DFPases), a subclass of the phosphotriesterases, are enzymes capable of hydrolysing organophosphorus nerve agents. To date, no three-dimensional structure of a `squid-type' DFPase is known. Here, the crystallization of the DFPase originally isolated from head ganglion of the squid Loligo vulgaris is reported. The protein has been heterologously expressed in Escherichia coli, purified to homogeneity and subsequently crystallized. The protein crystals belong to space group P212121, with unit-cell parameters a = 43.1, b = 82.1, c = 86.6 Å and one monomer per asymmetric unit. Under cryoconditions (120 K) the crystals diffracted beyond 2.0 Å using a Cu rotating-anode X-ray generator.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444900014232
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