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  • 2000-2004  (116)
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  • 1
    Electronic Resource
    Electronic Resource
    TGFB1 gene polymorphisms and inflammatory bowel disease (2000)
    Springer
    Immunogenetics 51 (2000), S. 869-872 
    Details
    ISSN: 1432-1211
    Keywords: Inflammatory bowel disease Crohn's disease Ulcerative colitis Transforming growth factor Polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510000211
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  • 2
    Electronic Resource
    Electronic Resource
    Solvent behaviour in flash-cooled protein crystals at cryogenic temperatures (2001)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. 566-573 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The solvent behaviour of flash-cooled protein crystals was studied in the range 100–180 K by X-ray diffraction. If the solvent is within large channels it crystallizes at 155 K, as identified by a sharp change in the increase of unit-cell volume upon temperature increase. In contrast, if a similar amount of solvent is confined to narrow channels and/or individual cavities it does not crystallize in the studied temperature range. It is concluded that the solvent in large channels behaves similarly to bulk water, whereas when confined to narrow channels it is mainly protein-associated. The analogy with the behaviour of pure bulk water provides circumstantial evidence that only solvent in large channels undergoes a glass transition in the 100–180 K temperature range. These studies reveal that flash-cooled protein crystals are arrested in a metastable state up to at least 155 K, thus providing an upper temperature limit for their storage and handling. The results are pertinent to the development of rational crystal annealing procedures and to the study of temperature-dependent radiation damage to proteins. Furthermore, they suggest an experimental paradigm for studying the correlation between solvent behaviour, protein dynamics and protein function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444901001196
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  • 3
    Electronic Resource
    Electronic Resource
    THE ROLE OF THE MICROSOMAL TRIGLYGERIDE TRANSFER PROTEIN IN ABETALIPOPROTEINEMIA (2000)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Nutrition 20 (2000), S. 663-697 
    Details
    ISSN: 0199-9885
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The microsomal triglyceride transfer protein (MTP) is a dimeric lipid transfer protein consisting of protein disulfide isomerase and a unique 97-kDa subunit. In vitro, MTP accelerates the transport of triglyceride, cholesteryl ester, and phospholipid between membranes. It was recently demonstrated that abetalipoproteinemia, a hereditary disease characterized as an inability to produce chylomicrons and very low-density lipoproteins in the intestine and liver, respectively, results from mutations in the gene encoding the 97-kDa subunit of the microsomal triglyceride transfer protein. Downstream effects resulting from this defect include malnutrition, very low plasma cholesterol and triglyceride levels, altered lipid and protein compositions of membranes and lipoprotein particles, and vitamin deficiencies. Unless treated, abetalipoproteinemic subjects develop gastrointestinal, neurological, ophthalmological, and hematological abnormalities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.nutr.20.1.663
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of organic versus conventional arable farming on soil structure and organic matter dynamics in a marine loam in the Netherlands (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 19 (2003), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. We compared the effects of conventional and organic arable farming on soil organic matter (SOM) content, soil structure, aggregate stability and C and N mineralization, which are considered important factors in defining sustainable land management. Within one soil series, three different farming systems were selected, including a conventional and an organic arable system and permanent pasture without tillage. The old pasture represents optimal conditions in terms of soil structure and organic matter inputs and is characterized by high earthworm activity. More than 70 years of different management has caused significant differences in soil properties. SOM content, mineralization, earthworm activity and water-stable aggregation decreased as a result of tillage and arable cropping when compared with pasture, but were significantly greater under organic farming than under conventional farming. Total SOM contents between 0 and 20 cm depth amounted to 15, 24 and 46 g kg−1 for the conventional arable, organic arable and permanent pasture fields, respectively. Although less sensitive to slaking than the conventionally managed field, the soil under organic farming was susceptible to compaction when high pressures were exerted on the soil under wet conditions. The beneficial effects of organic farming are generally associated with soil biochemical properties, but soil physical aspects should also be considered. Depending on soil type and climate, organic farmers need to be careful not to destroy the soil structure, so that they can enjoy maximum advantage from their organic farming systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2003.tb00297.x
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  • 5
    Electronic Resource
    Electronic Resource
    Refining soil survey information for a Dutch soil series using land use history (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 18 (2002), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Differences in land-use history within soil series, although not influencing soil classification, lead to variability of non-diagnostic soil properties in soil databases. Regional studies that use soil databases are confronted with this considerable variability. This has, for example, been reported in regional studies focused on nitrate leaching from agricultural land. Such findings have a direct impact on regional assessments of nitrate leaching from dairy farms on sandy soils, a major environmental issue in the Netherlands. There is thus a need to deal with this variability in soil properties.We were able to relate soil organic nitrogen, soil organic carbon and its dynamics to land use history for a Dutch sandy soil series. Within one soil series, three different land use histories were identified: old grassland, reseeded grassland and grassland converted from continuous cropping with silage maize. The addition of landscape characteristics significantly improved the regression models based on land-use only. Once established for any given soil series, such relationships can significantly improve soil survey input into dynamic models of soil behaviour such as regional nitrate leaching studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2002.tb00235.x
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  • 6
    Electronic Resource
    Electronic Resource
    The role of soil science in the land use negotiation process (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 17 (2001), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. The hierarchial concept of land use planning becomes less relevant in a society with continuous interactions between stakeholders, researchers, planners and politicians. In this context, land use negotiation rather than land use planning appears to be the most appropriate concept. In the negotiation process, good quality data about the land is important as land properties are, obviously, key elements to be considered. Case studies at farm and regional level have been analysed to explore answers to a number of questions. How can soil data be presented most effectively? What are the research needs? How can the large existing body of data be mobilized most effectively? Studies on regional land use in Costa Rica used methods in a logical sequence including projections, explorations and predictions of land use patterns. The work involved upscaling of data, obtained at farm level, to the regional level. Work at farm level focussed on prototyping procedures in which farming systems were ‘designed’ by close interaction between farmers and scientists, including applications of precision agriculture. Soil data demands were analysed, emphasizing the effects of using data with different degrees of detail together with the application of pedotransfer functions which effectively transform existing data into parameters that are difficult or expensive to measure directly. This not only facilitated interactions with stakeholders but also with colleague scientists in interdisciplinary teams. In addition, use of Geographical Information Systems allowed visual presentations of alternative geographical land use patterns that were associated with various scenarios, thereby facilitating the interaction processes. A plea is made to increase interaction of stakeholders and researchers by considering research programmes as vehicles for joint learning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2001.tb00001.x
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  • 7
    Electronic Resource
    Electronic Resource
    Influence of temperature and soil drying on respiration of individual roots in citrus: integrating greenhouse observations into a predictive model for the field (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 24 (2001), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In citrus, the majority of fine roots are distributed near the soil surface – a region where conditions are frequently dry and temperatures fluctuate considerably. To develop a better understanding of the relationship between changes in soil conditions and a plant’s below-ground respiratory costs, the effects of temperature and soil drying on citrus root respiration were quantified in controlled greenhouse experiments. Chambers designed for measuring the respiration of individual roots were used. Under moist soil conditions, root respiration in citrus increased exponentially with changes in soil temperature (Q10 = 1·8–2·0), provided that the changes in temperature were short-term. However, when temperatures were held constant, root respiration did not increase exponentially with increasing temperatures. Instead, the roots acclimated to controlled temperatures above 23 °C, thereby reducing their metabolism in warmer soils. Under drying soil conditions, root respiration decreased gradually beginning at 6% soil water content and reached a minimum at 〈2% soil water content in sandy soil. A model was constructed from greenhouse data to predict diurnal patterns of fine root respiration based on temperature and soil water content. The model was then validated in the field using data obtained by CO2 trapping on root systems of mature citrus trees. The trees were grown at a site where the soil temperature and water content were manipulated. Respiration predicted by the model was in general agreement with observed rates, which indicates the model may be used to estimate entire root system respiration for citrus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2001.00723.x
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  • 8
    Electronic Resource
    Electronic Resource
    Soil Organic Matter Content as a Function of Different Land Use History (2000)
    Springer
    Soil Science Society of America journal 64 (2000), S. 689-693 
    Details
    ISSN: 1435-0661
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: −1 . Regression models of the actual SOM content as a function of crop type and management in the different periods showed that SOM contents were increased under long-term grass or, to a lesser extent, by organic farming, when compared with conventional-arable use. The regression model depends on the nature of land use history in any particular region and on the length of the selected periods, but it provides an easy method to predict SOM content as a function of management in a given soil series. The method can be an alternative to simulation modeling in situations where detailed data records from long-term field experiments are not available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/
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  • 9
    Electronic Resource
    Electronic Resource
    High aquatic niche overlap in the newts Triturus cristatus and T. marmoratus (Amphibia, Urodela) (2000)
    Springer
    Hydrobiologia 437 (2000), S. 149-155 
    Details
    ISSN: 1573-5117
    Keywords: aquatic niche overlap ; microsatellites ; newts ; Triturus cristatus ; Triturus marmoratus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied spatial niche metrics of large-bodied newts (Triturus cristatus and T. marmoratus) in three breeding ponds in western France. Adults and larvae were sampled with underwater funnel traps. Larvae were identified to the species with diagnostic microsatellite DNA markers. The distribution of adult T. cristatus and T. marmoratus across pond regions differed in one out of six cases, no differences were observed between larvae (two ponds studied). Niche overlap and niche breadth indices across resource states defined as pond regions or individual traps were high (Schoener's C: pond regions 0.60–0.98, traps 0.35–0.71; Levins' B: pond regions 0.71–0.98, traps 0.35–0.76). Adults of large-bodied newts significantly differed in resource use from small-bodied newts (T. helveticus). The results are discussed in view of the occurrence of interspecific breeding attempts, and the unpredictable ecological characteristics of newt breeding ponds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026554907400
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  • 10
    Electronic Resource
    Electronic Resource
    Sample preparation and scanning protocol for computerised analysis of root length and diameter (2000)
    Springer
    Plant and soil 218 (2000), S. 185-196 
    Details
    ISSN: 1573-5036
    Keywords: root architecture ; root-diameter distribution ; root efficiency ; root length ; root scanning ; Delta-T Scan ; WinRHIZO
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Root length and diameter distribution are important characteristics to be considered when describing and comparing root systems. Root length and root-diameter distribution may be obtained in two ways: by microscopical measurements, which are laborious, or by computerised analysis, which is fast but sensitive to the scanning protocol. Although scanning protocols vary widely between laboratories, papers rarely report the details. Using two commercially available root analysis software packages (Delta-T Scan and WinRHIZO), we performed a sensitivity analysis of scanning protocols for estimating root length and diameter distribution. The results are shown graphically, rather than numerically, because the interactions between different parameters in the overall scanning protocol are most clearly illustrated by the shape of the curves. The present analysis clearly demonstrated the sensitivity of the two scanning methods with regard to staining period, maximum root density, scanning resolution and transformation threshold. For example, estimating the root-diameter distribution versus measuring root length, puts opposite constraints on the transformation threshold settings. We suggest the following settings for the most sensitive parameters: a staining period of 24 h, a root density of less than 0.5 mm root per mm2 surface, a resolution of 400 dpi and the automatic threshold for WinRHIZO and a brightness of 200 for Delta-T Scan. According to this protocol, comparison of computerised analyses with microscopic measurements showed good total root length and diameter distribution agreement for three contrasting root systems. We suggest to always start with the present protocol when studying other species. If validation indicates that the proposed scanning protocol needs to be modified for other species, the present sensitivity analysis may be used as a guideline for changing the most critical parameters. Similarly, the use of another stain than neutral red may also require modifications of the scanning protocol. In general, a long staining period (e.g., 24 h) is recommended for all stains, as small differences in staining period have the least effect when the root tissue is saturated. To enhance comparability of results in the literature, the staining period, stain, (maximum) root density, scanner resolution and threshold should always be listed when root data obtained by scanning are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1014905104017
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