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  • 1
    Electronic Resource
    Electronic Resource
    Detection of Xylophilus ampelinus in grapevine cuttings using a nested polymerase chain reaction (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 50 (2001), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A sensitive and specific assay was developed to detect bacterial blight of grapevine caused by Xylophilus ampelinus (Panagopoulos, 1969) comb. nov. in grapevine cuttings. The 16S−23S rDNA intergenic spacer region of X. ampelinus was sequenced and pathogen-specific primers were designed from a region in the spacer between the tRNA (Ala) and the 23S genes. A nested PCR (n-PCR) reaction was applied with a first-stage PCR using universal primers within the ends of the 16S and 23S genes, followed by a second-stage PCR with nested primers specific to the X. ampelinus spacer region. A 277-bp fragment was amplified from 38 Xylophilus strains tested, but not from saprophytes associated with grapevine or phylogenetically related phytobacteria. The 277-bp product was shown to be derived from the X. ampelinus spacer region by restriction with Dra I, Sau 3AI, Taq I and Msp I, Southern hybridization and genomic DNA dot blots. When the (n-PCR) procedure was applied in the absence of nontarget DNA, the limit of detection was less than 10 colony-forming units (CFU) per µL. The same number of X. ampelinus CFU could be detected in the presence of 1·5 × 105 CFU µL−1 of Erwinia herbicola cells using the n-PCR procedure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2001.00568.x
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  • 2
    Electronic Resource
    Electronic Resource
    Promoter analysis and transcription profiling: Integration of genetic data enhances understanding of gene expression (2004)
    Oxford, UK; Malden , USA : Munksgaard International Publishers
    Physiologia plantarum 120 (2004), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: It is increasingly evident that transcription control might be conserved among organisms. For this reason, genome sequencing and gene expression profiling methods, which have yielded a plethora of data in different organisms, may be applied in species where genomic sequence is limited to mostly expression array and EST data. The identification of transcription factors and promoters associated with gene expression profiles and ESTs could therefore contribute to elucidate and predict complex regulatory events in plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0031-9317.2004.0209.x
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  • 3
    Electronic Resource
    Electronic Resource
    Carbon allocation to the insoluble fraction, respiration and triose-phosphate cycling in the sugarcane culm (2002)
    Oxford, UK : Blackwell Science, Ltd
    Physiologia plantarum 116 (2002), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The changes in carbon allocation to non-sucrose metabolic pathways were investigated in developing internodes of sugarcane. Radiolabelling studies were done on internode 3, 6 and 9 tissues, representing three stages of increasing maturity. Carbon partitioning into sucrose increased from 34% of total 14C uptake in internode 3, to 66% in internodes 9. In immature tissue, the protein and fibre components were the dominant competing sinks with sucrose for incoming carbon, to which 14 and 16% of carbon was allocated. Increased carbon allocation to sucrose with tissue maturity coincided with a decrease in partitioning to fibre and total respiration. Between internodes 3 and 9 carbon allocation to total respiration decreased by 9%, and to fibre by 14%. Carbon cycling between the triose- and hexose phosphate pools was evident in all internodes. More than 90% of carbon entering triose-phosphates was returned to hexose in internode 3 tissue, and this flux decreased with tissue maturity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.2002.1160102.x
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  • 4
    Electronic Resource
    Electronic Resource
    Protein-level expression and localization of sucrose synthase in the sugarcane culm (2004)
    Oxford, UK; Malden , USA : Munksgaard International Publishers
    Physiologia plantarum 121 (2004), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: No comprehensive studies on the localization of sucrose synthase (SuSy, EC 2.4.1.13) in sugarcane internodes have been reported. The expression and localization of SuSy in young (internode 3) to mature (internode 9) internodes of sugarcane (Saccharum spp. hybrids) var. N19 was investigated. Enzyme activity in the top and bottom, as well as the peripheral and core parts of the internodes suggested that SuSy is present ubiquitously but that levels can differ significantly in different parts of the internodes and with maturity. This was also confirmed by immunohistochemistry, which showed that both vascular and storage parenchyma tissues contain SuSy in young and mature internodes. The ratio of sucrose breakdown to synthesis activity increased approximately 12-fold from an average of 0.12 in internode three to 1.4 in internode nine. This indicates that different forms of SuSy are present in young and mature internodes, or that the ratios of different isoforms differ between young and mature internodes. Immunoblotting showed that at least one form of SuSy present in young tissue was absent, or present below detection limits, in mature culm tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0031-9317.2004.00316.x
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  • 5
    Electronic Resource
    Electronic Resource
    Aphid (Sitobion yakini) investigation suggests thin-walled sieve tubes in barley (Hordeum vulgare) to be more functional than thick-walled sieve tubes (2002)
    Oxford, UK : Blackwell Science, Ltd
    Physiologia plantarum 115 (2002), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Barley, like most other grasses that have been studied, contains two kinds of sieve tube. The first formed are called thin-walled sieve tubes because of their thin wall compared to the late-formed, and are associated with companion cells. The late-formed are thick-walled sieve tubes, which differentiate next to the metaxylem vessels and lack companion cells. Aphid (Sitobion yakini (Eastop) feeding was studied using light microscopy to determine if they preferentially feed from thin- or thick-walled sieve tubes in the barley leaf. Penetration of the stylets through the leaf epidermis and mesophyll was largely intercellular, becoming partly intercellular and, partly, intracellular inside the vascular bundle. Sixteen of 19 pairs of stylets (84%), and 293 of 317 (92%) stylet tracks terminated at the thin-walled sieve tubes, suggesting that Sitobion yakini feeds preferentially on the thin-walled sieve tubes which seem to be more attractive to the aphid. These thin-walled sieve tubes are thus probably the most functional in terms of phloem loading and transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.2002.1150116.x
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic effects and heterosis of flowering and fruit characteristics of tropical pumpkin (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Tropical pumpkin is an important vegetable in many lowland tropics. Estimation of genetic effects for a fixed set of genotypes may yield information on the presence of different types of gene action for important traits. Six landraces and one diallel set of their progeny were evaluated for time from planting to female anthesis and fruit characteristics. Variety effects and heterosis mean squares were significant for the days to female anthesis, the weight of the first mature fruit, the mean fruit weight and soluble solids, indicating the presence of both additive and non-additive gene actions. Only variety effects were significant for the days to the first mature fruit. These characters could be improved by recurrent selection. Selection for heterosis would be effective except for the days to the first fruit to mature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00595.x
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  • 7
    Electronic Resource
    Electronic Resource
    The utilization of trend analysis in the effective monitoring of information security. Part 1: the concept (2001)
    Bradford : Emerald
    Information management & computer security 9 (2001), S. 237-242 
    Details
    ISSN: 0968-5227
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Computer Science
    Notes: A survey recently completed by the Computer Security Institute and the Federal Bureau of Investigation revealed that corporations, banks, and governments all face a growing threat from computer crime, and in particular computer hacking. The computer hacking activities caused well over $100 million in losses last year in the USA and the trend toward professional computer crime, such as computer hacking, is on the rise. Different methods are currently used to control the computer crime problem, for example, to control the access to and from a network by implementing a firewall. As the survey highlighted, most of these methods are inefficient. New means and ways which will minimise and control the hacking problem must therefore continuously be researched and defined. This paper will propose a method, based on trend analysis, that could be utilized to minimise and control the hacking problem in an organisation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/EUM0000000006069
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  • 8
    Electronic Resource
    Electronic Resource
    The utilization of trend analysis in the effective monitoring of information security. Part 2: the model (2002)
    Bradford : Emerald
    Information management & computer security 10 (2002), S. 5-11 
    Details
    ISSN: 0968-5227
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Computer Science
    Notes: A survey recently completed by the Computer Security Institute (CSI) and the Federal Bureau of Investigation (FBI) revealed that corporations, banks, and governments all face a growing threat from computer crime, and in particular computer hacking. Computer hacking activities caused well over US$100 million in losses last year in the USA and the trend toward professional computer crime, such as computer hacking, is on the rise. Different methods are currently used to control the computer crime problem, for example, by controling access to and from a network by implementing a firewall. As the survey highlighted, most of these methods are insufficient. New means and ways which will minimise and control the hacking problem must therefore continuously be researched and defined. Proposes a method, using trend analysis, that could be utilized to minimise and control the hacking problem in an organisation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/09685220210417454
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  • 9
    Electronic Resource
    Electronic Resource
    A framework for access control in workflow systems (2001)
    Bradford : Emerald
    Information management & computer security 9 (2001), S. 126-133 
    Details
    ISSN: 0968-5227
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Computer Science
    Notes: Workflow systems are often associated with business process re-engineering (BPR). This paper argues that the functional access control requirements in workflow systems are rooted in the scope of a BPR project. A framework for access control in workflow systems is developed. The framework suggests that existing role-based access control mechanisms can be used as a foundation in workflow systems. The framework separates the administration-time and the run-time aspects. Key areas that must be investigated to meet the functional requirements imposed by workflow systems on access control services are identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/09685220110394848
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  • 10
    Electronic Resource
    Electronic Resource
    A cyclic approach to business continuity planning (2004)
    Bradford : Emerald
    Information management & computer security 12 (2004), S. 328-337 
    Details
    ISSN: 0968-5227
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Computer Science
    Notes: In a world where continuous operations are essential for business survival, action must be taken to ensure that information and the business processes that use the information are continuously available. This usually involves the selection and implementation of a suitable business continuity plan. Implementing such a plan is, however, not always a simple task. This especially holds true for small to medium-sized organizations. An implementation method that could be applied to most business continuity planning methodologies would, therefore, be a welcome tool, especially for small to medium-sized organisations. This paper presents a theoretical model for such an implementation method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/09685220410553541
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