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  • Articles  (17)
  • 2000-2004  (16)
  • 1955-1959  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Promoters derived from banana bunchy top virus DNA-1 to −5 direct vascular-associated expression in transgenic banana (Musa spp.) (2000)
    Springer
    Plant cell reports 19 (2000), S. 810-814 
    Details
    ISSN: 1432-203X
    Keywords: Key words Banana bunchy top virus ; Promoter ; Vascular expression ; Green fluorescent protein ; Intron-mediated enhancement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The intergenic regions of banana bunchy top virus (BBTV) DNA-1 to -5 were fused to the green fluorescent protein (GFP) and uidA reporter genes and assessed for promoter activity in transgenic banana (Musa spp. cv. Bluggoe). Promoter activity associated with the BBTV-derived promoters was transgene dependent with greatest activity observed using the GFP reporter. The BBTV promoters (BT1 to BT5) directed expression primarily in vascular-associated cells, although levels of activity varied between individual promoters. Promoters BT4 and BT5 directed the highest levels of GFP expression, while activity from BT1, BT2 and BT3 promoters was considerably weaker. Intron-mediated enhancement, using the maize polyubiquitin 1 (ubi1) intron, generated a significant increase in GUS expression directed by the BBTV promoters in transgenic plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002999900185
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  • 2
    Electronic Resource
    Electronic Resource
    Linkage mapping and QTL analysis in coconut (Cocos nucifera L.) (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 292-300 
    Details
    ISSN: 1432-2242
    Keywords: Key words AFLP ; DNA markers ; Early germination ; ISSR ; ISTR ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Different DNA marker types were used to construct linkage maps in coconut (Cocos nucifera L.; 2n = 32) for the two parents of the cross Malayan Yellow Dwarf (MYD) × Laguna Tall (LAGT). A total of 382 markers was sufficient to generate 16 linkage groups for each parent. The total genome length corresponded to 2226 cM for the LAGT map and 1266 cM for the MYD map with 4–32 markers per linkage group. Common markers allowed the association of 9 linkage groups for the two parents MYD and LAGT. QTL analysis for the trait early germination identified six loci. These QTLs correlate with early flowering and yield, representing characters which are important in coconut breeding. The co-segregation of markers with these QTLs provides the first opportunity for marker-assisted selection in coconut breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051482
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  • 3
    Electronic Resource
    Electronic Resource
    High-yielding repetitive somatic embryogenesis and plant recovery in a selected tea clone, ‘TRI-2025’, by temporary immersion (2000)
    Springer
    Plant cell reports 19 (2000), S. 1140-1145 
    Details
    ISSN: 1432-203X
    Keywords: Keywords Multiplication ; TIS ; Propagation ; ¶Secondary embryogenesis ; Tissue culture ; Regeneration ; AbbreviationsSEM Somatic embryos ; PGR Plant growth regulators ; TIS Temporary immersion system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Methods for improving the efficiency of repetitive somatic embryogenesis and plant recovery from somatic embryos of clonal tea, TRI 2025 [Camellia sinensis (L.) O. Kuntze] were investigated by optimising the immersion frequencies of the explants using a modified temporary immersion system (TIS). The relative efficiencies of three conventional methods for multiplying embryos were compared with the temporary immersion method. The highest rate of multiplication of secondary embryos (24-fold) was achieved using the TIS. By controlling the immersion cycles, we achieved more consistent, synchronised multiplication and embryo development with a high level of plant recovery. A one-step computer-programmed immersion protocol based on a single, simple medium with no growth regulators was developed, enabling multiplication, maturation, germination and plant recovery within 17 weeks. Plantlets recovered through this method were hardy, with 2- to 5-cm-long shoots containing a minimum of 2–4 lush green leaves and a well-formed taproot. Callus formation, hyperhydricity and other developmental abnormalities were not observed at any stage in the process. Plantlets produced using this method were successfully acclimatised to glasshouse conditions. This protocol avoids culture transfers, and thus minimises the risk of contamination and reduces labour costs. This technique could have significant commercial implications in tea propagation as it has the potential for large-scale production with considerably reduced production costs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000239
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic transformation of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment (2000)
    Springer
    Plant cell reports 19 (2000), S. 229-234 
    Details
    ISSN: 1432-203X
    Keywords: Key words Musa ; Banana ; Transformation ; Microprojectile bombardment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotransferase (nptII) selectable marker gene under the control of a banana bunchy top virus (BBTV) promoter or the CaMV 35S promoter, and either the β-glucuronidase (uidA) reporter gene or BBTV genes under the control of the maize polyubiquitin promoter. Plants were regenerated, under selection with kanamycin, that were co-transformed with nptII and either the uidA or BBTV genes. Molecular characterisation of transformants demonstrated that the transgenes had been stably integrated into the banana genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050004
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic engineering of cereal crop plants: a review (1955)
    Springer
    Euphytica 85 (1955), S. 35-44 
    Details
    ISSN: 1573-5060
    Keywords: cereals ; protoplast transformation ; tissue electroporation ; particle bombardment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Many aspects of basic and applied problems in plant biology can be investigated by transformation techniques. In dicotyledonous species, the ability to generate transgenic plants provides the tools for an understanding of plant gene function and regulation as well as for the directed transfer of genes of agronomic interest. For many dicotyledonous plants Agrobacterium tumefaciens can be routinely used to introduce foreign DNA into their genome. However, cereals seem to be recalcitrant to Agrobacterium-mediated transformation. In cereals, many efforts have been made in recent years to establish reliable transformation techniques. Several transformation techniques have been developed but to date only three methods have been found to be suitable for obtaining transgenic cereals: transformation of totipotent protoplasts, particle bombardment of regenerable tissues and, more recently, tissue electroporation. The current state of transformation methods used for cereals will be reviewed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023928
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  • 6
    Electronic Resource
    Electronic Resource
    Use of Nuclear Track Detection Technique to Study Boron and Nitrogen Distributions in Alloys (2000)
    Springer
    Journal of radioanalytical and nuclear chemistry 244 (2000), S. 435-439 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The paper is devoted to determination of boron and nitrogen in metallic materials by nuclear track techniques for the case of high background in radiograms. The method is based on the detection of the 10B(n,α)7Li and 14N(n,p)14C reaction products with plastic track detectors exposed to a neutron flux in a close contact with the samples analyzed. When determining boron, high background can arise due to fast neutron interactions with hydrogen existing in the detector material and with matrix elements of the sample. In the case of nitrogen determination, high background is usually associated with a presence of boron in metals and alloys, alpha tracks of which strongly interfere with proton tracks from the 14N(n,p)14C reaction. The feasibility for measurement of micro-concentrations of boron as well as nitrogen determination in the presence of boron has been studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006716500517
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  • 7
    Electronic Resource
    Electronic Resource
    30 Years of Reactor Characterization on the NBSR (2000)
    Springer
    Journal of radioanalytical and nuclear chemistry 244 (2000), S. 361-365 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The NIST nuclear reactor irradiation facilities used for neutron activation analysis have been well characterized for absolute neutron flux measurements, variations within each irradiation facility, neutron energy measurements of each facility, and temperature and pressure measurements. This has been accomplished with various sample materials and under a range of conditions including biological samples inside of sealed quartz ampoules, and for radiation damage measurements on the polyethylene rabbits used for all pneumatic tube irradiations. As a result of all these measurements, initiated in 1969 and still continuing, the NBSR irradiation facilities have become the most highly characterized NAA irradiation facilities in the world. This paper describes the methods and techniques developed and used for characterization of the NBSR irradiation facilities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006735609177
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  • 8
    Electronic Resource
    Electronic Resource
    Determination of Ultratrace Levels of Mercury in Three SRMs by Combustion RNAA (2000)
    Springer
    Journal of radioanalytical and nuclear chemistry 245 (2000), S. 91-95 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A radiochemical neutron activation analysis (RNAA) combustion method coupled with a neutron exposure normalization technique was used to determine low μg/kg mercury levels in three National Institute of Standards and Technology (NIST) Standard Reference Materials (SRMs). Two coals (sub-bituminous and bituminous) and a diet material were analyzed. The results obtained provided recommended values of approximately 5 μg/kg for SRM 1548a Typical Diet, 24 μg/kg for SRM 1635 Trace Elements in Coal (sub-bituminous), and 100 μg/kg for SRM 1632b Trace Elements in Coal (bituminous).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006775702465
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  • 9
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    Nonlinear partial differential equations and applications: Involvement of estrogen receptor   in terminal differentiation of mammary gland epithelium (2002)
    National Academy of Sciences
    Details
    Publication Date: 2002-11-18
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 10
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    From cell death to embryo arrest: Mathematical models of human preimplantation embryo development (2001)
    National Academy of Sciences
    Details
    Publication Date: 2001-02-13
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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