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  • 1
    Electronic Resource
    Electronic Resource
    Effect of Supplementing Ascorbic Acid (L-ascorbyl-2-polyphosphate) in Broodstock Diet of the White Shrimp Litopenaeus vannamei (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 31 (2000), S. 0 
    Details
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-7345.2000.tb00709.x
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  • 2
    Electronic Resource
    Electronic Resource
    Voluntary Spawning, Early Development, and Completion of the Life Cycle of Spotted Sand Bass Paralabrax maculatofasciatus in the Laboratory (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 32 (2001), S. 0 
    Details
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract.— Spawning behavior and development of spotted sand bass Paralabrax maculatofasciatus were studied in the laboratory. Captive fish (15–20 cm standard length) spawned in 100-L aquaria at 24 C and 35 ppt salinity with a controlled photoperiod (13 h light: 11 h dark). Distinct courtship coloration and displays were observed. Courtship began near noon and continued all afternoon. Spawning occurred toward the surface during late afternoon. Development from fertilization to 3 d after hatching is described. Hatching occurred in 24–25 h at 24 C. Larvae were reared in 100-L aquaria with microalgae, rotifers, and Artemia. First feeding occurred 3 d after hatching, and 5.3% survival was obtained at 17 d (4.1-mm mean notochord length, 1.7–5.5 mm range). On a diet of minced clams and fish, first maturity was reached at 7.5 mo (19.5 g mean weight, 8.3–37.9 g range and 90 mm mean standard length, 66–116 mm range).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-7345.2001.tb00931.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of Stocking Density on the Growth and Survival of Spotted Sand Bass Paralabrax maculatofasciatus Larvae in a Closed Recirculating System (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 32 (2001), S. 0 
    Details
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract.— The effect on growth and survival of the initial stocking density (50, 100, 150, and 200 larvae/ L) in larval rearing of spotted sand bass was evaluated over 30 d in a closed recirculating system. Larvae were fed with rotifers, copepods, nauplii and adult Artemia, and spotted sand bass yolk-sac larvae. Water quality was monitored daily. The notochordal or standard length of sampled larvae was measured by image analysis. Specific growth rates at each density were compared by covariance analysis. Survival was estimated from day 15 to the end of the experiment, when a resistance test was used to evaluate the juvenile quality among densities. At the end of the experiment, mean standard length of larvae at lower densities was significantly larger (P 〈 0.05) than at higher densities. Higher specific growth rates were found at lower densities. Significantly higher survival (P 〈 0.05) was recorded for the lowest density, but the highest number of harvested fish was obtained with the highest densities (150 and 200 larvae/L). The lowest density also showed the significantly (P 〈 0.05) higher survival after using a resistance test. We conclude the highest density can be used in larval rearing of spotted sand bass. However, better survival, growth, and seed quality are obtained at the lowest density. To recommend an optimal density for this specie, it is necessary to improve the water quality in the culture system and to make a cost-benefit study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-7345.2001.tb00932.x
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  • 4
    Electronic Resource
    Electronic Resource
    Ribonuclease U2: cloning, production in Pichia pastoris and affinity chromatography purification of the active recombinant protein (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 189 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: RNase U2 is an endoribonuclease secreted by the fungus Ustilago sphaerogena. Its genomic DNA (rnu2), containing an intron of 116 bp, has been isolated and cloned. The corresponding cDNA has also been synthesized. The recombinant RNase U2 was successfully produced in Pichia pastoris, fused to the yeast alkaline phosphatase signal peptide. The recombinant RNase U2, purified by affinity chromatography, contains three extra amino acids at its amino-terminal end and retains the enzymatic and spectroscopic properties of the natural fungal protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09224.x
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  • 5
    Electronic Resource
    Electronic Resource
    The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 191 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We report cloning and sequencing of gene ps1 encoding a versatile peroxidase combining catalytic properties of lignin peroxidase (LiP) and manganese peroxidase (MnP) isolated from lignocellulose cultures of the white-rot fungus Pleurotus eryngii. The gene contains 15 putative introns, and the deduced amino acid sequence consists of a 339-residue mature protein with a 31-residue signal peptide. Several putative response elements were identified in the promoter region. Amino acid residues involved in oxidation of Mn2+ and aromatic substrates by direct electron transfer to heme and long-range electron transfer from superficial residues as predicted by analogy with Phanerochaete chrysosporium MnP and LiP, respectively. A dendrogram is presented illustrating sequence relationships between 29 fungal peroxidases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09316.x
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  • 6
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    Wetland restoration and nitrate reduction: the example of the peri-urban wetland of Vitoria-Gasteiz (Basque Country, North Spain) (2003)
    Copernicus
    Details
    Publication Date: 2003-02-28
    Description: Changes in land use and agricultural intensification caused wetlands on the quaternary aquifer of Vitoria-Gasteiz (Basque Country) to disappear some years ago and nitrate concentration in groundwaters increased very quickly. The Basque Government recently declared the East Sector of this aquifer a Vulnerable Zone according to the 91/676/CEE European Directive. Recently, the wetlands have been restored through the closure of the main drainage ditches, the consequent elevation of the water table and the abondonment of agricultural practices near the wetlands. This is the case of the Zurbano wetland. Restoration has allowed the recovery of its biogeochemical function, which has reduced nitrate concentrations in waters. Nitrate concentrations which exceed 50 mg l–1 in groundwaters entering into the wetland are less than 10 mg l–1 at the outlet. Conditions in the wetland are conducive to the loss of nitrates: organic matter rich wetted soils, clay presence allowing a local semiconfined flow and very low hydraulic gradient. Water quality monitoring at several points around the wetland showed the processes involved in nitrate loss, although some aspects still remain unresolved. However, during storm events, the wetland effectively reduces the nitrate concentration entering the Alegria River, the most important river on the quaternary aquifer. Keywords: restored wetland functionality, nitrate losses, Basque Country
    Print ISSN: 1027-5606
    Electronic ISSN: 1607-7938
    Topics: Geography , Geosciences
    Published by Copernicus on behalf of European Geosciences Union.
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  • 7
    Electronic Resource
    Electronic Resource
    New communication systems and trade union politics: a case study of Spanish trade unions and the role of the internet (2003)
    Oxford, UK and Boston, USA : Blackwell Publishing Ltd
    Industrial relations journal 34 (2003), S. 0 
    Details
    ISSN: 1468-2338
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Notes: This article shows how engagement with the internet and aspects of the new economy/society require an appreciation of union difference and politics. It shows how union responses vary due to four factors: communication strategies, union identity, forms of internal democracy and a range of organisational and social contingencies. The article will study the phenomena of the internet in the context of one national case study, Spain. It is a country that allows us to view the political dimensions of the internet, and the way they link to communication strategies, due to the manner in which the labour movement has transformed and modernised itself. It is also of interest because methods of communication have been at the heart of the way in which unions have developed and differentiated themselves. This article will argue that a greater sensitivity is required in terms of union history, politics and identity if the impact and use of new forms of communication are to be fully appreciated. It also points to the need to appreciate tensions between different forms of communication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/1468-2338.00279
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  • 8
    Electronic Resource
    Electronic Resource
    Residual effects of pig slurry applied to a Mediterranean soil on yield and N uptake of a subsequent wheat crop (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 20 (2004), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. In areas of intensive pig farming, fresh pig slurry is often applied annually to the same fields. Thus, to avoid nitrogen (N) losses correct fertilizer practice should take account of residual effects of slurry on the following crops. The residual effects of different rates of slurry applied during three years were evaluated in subsequent wheat crops. The experiment was conducted on an irrigated Mediterranean Typic Xerofluvent soil, where plots were left unfertilized or fertilized with 150 kg N ha−1 as ammonium nitrate. Grain yield and grain N uptake increased with slurry rates in both fertilized and unfertilized treatments. The increases in the unfertilized treatments were interpreted as a nitrogen effect of the previous 1996–98 slurry applications. The equivalent mineral N released from the pig slurry was underestimated by two existing decay-series approaches. Although decay-series are useful tools for estimating manure residual effects they should be adjusted for local conditions. A significant positive relationship was detected between apparent N use efficiency of the slurry and the total amount of applied organic N, which was interpreted as a specific residual effect rather than due to the N dose of previously applied pig slurry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2004.tb00351.x
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  • 9
    Electronic Resource
    Electronic Resource
    Nutrient fluxes from a soil treatment process for pig slurry (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 16 (2000), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. The effects of pig slurry applications to a hydrologically isolated field treatment plant (at Solepur) were studied over a period of eight years. Thirty repeated doses, averaging 160 m3 ha−1 were applied from April to October of each year (1991–1995), to reach a total application of 4930 m3 ha−1. All slurry samples were analysed for their total solids (TS), macronutrient (C, N, P, K, Ca) and micronutrient (Cu, Zn) content. In total, 284 tonnes of total solids (57 t TS ha−1 yr−1), 115 tonnes of carbon (23 t C ha−1yr−1), 24.5 tonnes of nitrogen (4900 kg N ha−1 yr−1), 7964 kg of phosphorus (1593 kg P ha−1 yr−1), 16 518 kg of potassium (3304 kg K ha−1 yr−1), 183 kg copper (37 kg Cu ha−1 yr−1) and 266 kg zinc (53 kg Zn ha−1 yr−1) were applied to the soil. Thus, this site provides an opportunity to assess the balance and to examine the long-term behaviour of nutrients under conditions of intensive land application of pig slurries or similar effluents.The main nutrient fluxes through the soil-water system were determined for each element. Over 40% of the total carbon applied was retained by the soil. About 25% of the slurry nitrogen applied remained in the soil profile and 12.5% was leached through the drainage water as nitrate. Most of the slurry phosphorus applied was retained in the soil profile either as P-Dyer extractable (83%), or as total soil phosphorus (112%); 〈0.01% was found in the drainage water. Forty-three per cent of the potassium applied in the slurry was recovered from the soil profile and 15% was recovered in the drainage water. Most of the copper (62%) and zinc (74%) applied in the slurry remained in the soil as EDTA extractractable forms; very low percentages (0.05% and 0.6% respectively) were found in the drainage water.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2000.tb00183.x
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  • 10
    Electronic Resource
    Electronic Resource
    Dissolved reactive phosphorus in a Calcaric Fluvisol as affected by the addition of agricultural wastes (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 20 (2004), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Land application of organic wastes can result in the accumulation of available soil phosphorus (P) and in an increase risk of eutrophication of surface and shallow groundwaters. We conducted laboratory experiments to examine the effect of waste application on the concentration of dissolved reactive phosphorus (DRP) in 1:5 soil to 0.005 m CaCl2 suspensions. Ten organic wastes, of which eight were mature composts, were applied to a P-rich Calcaric Fluvisol at rates ranging from 0 to 10% of soil by weight, and the difference in DRP concentration between suspensions containing waste and the untreated control (ΔDRP) was measured over a period of 300 days. In half of the suspensions of each waste–soil combination, 80 mg P (as KH2PO4) kg−1 soil was applied at day 14. Values of ΔDRP were generally positive, but a significant number of negative values were also recorded for some wastes and application rates, particularly at later sampling times if inorganic P had been added. Regression analyses revealed that ΔDRP (i) increased with increasing soluble reactive P at all times and (ii) increased in the short term, but decreased in the long term with increasing water soluble organic carbon in the waste. The fact that ΔDRP was sometimes negative for some waste types suggests that application of organic waste to soil does not necessarily increase eutrophication risks caused by soil P losses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2004.tb00339.x
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